LOCUS       sequence-135338-       12199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deoxyviolacein pathway (vioABEC) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE) and 4A6 (vioC) for orthogonal flux redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12199)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 12199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC
FEATURES             Location/Qualifiers
     source          1..12199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1374..1418
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1471..1518
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    1571..1595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1610..1632
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4682..4726
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4779..4826
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    4879..4903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4918..4940
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5566..5610
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      5663..5710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    5763..5787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5802..5824
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7134..7178
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      7231..7278
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7322..7777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     7619..7640
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(7892..8683,8684..8752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8515..8534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8753..8845)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8926..9514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9415..9434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9668..9685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9700..9842
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9768..9787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9928..9950
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9944..10135)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(10707..11789)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     11751..11770
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(11790..11867)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     12073..12092
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgttaaat ttagagcatt cagtttcaat tgtcggagct ggaatttctg
      121 ggatagtgtg tgcaattaca ttagctcgtt caagggcatt agaaggttac gcaatagatg
      181 tctatgaaca aagcccccgc gttggtggac ggatccaaac aaacttgttg agcaatcatt
      241 acatcgattt aggtgccgct cgtttttgtt cacaattgca tggtaatgta tatgatctag
      301 tcaagaggtt tgaactcgaa tttgaaccgt ttccttttac tcaacttaga tttactcagc
      361 caattcaaaa agaactcaag actattttgt ctaatctaaa accgttaatc gatgaacata
      421 aagaagattc ttttgtcgaa tttgttacga gctatttggg taaagaaaaa gcaaaagcag
      481 taatcttagc actgggctac gactcactct ctatgccaat aatttctcca catattgctt
      541 acgatatcat tgaaaaacac ccagaaactc aatgtttcac tgaaaattgt gggaatgaat
      601 ggtttagttt gaaagaggga ttggcgtcgt tgatcaaggc gctatatgaa gaggcgttaa
      661 aactcgggat taagtttcat tttgaatcta cattagttca aataagcaat cgaggtaacg
      721 cccacagctt ggtgattgcc gataatcaaa atcaacaaaa agagatcagt aaagggctta
      781 agatcattac tttgcctcca tctcgaatgg aaaaattaga taagcaattt cctaaacgtt
      841 ggagcaacta caattatggt tctagcccct tgtttaaagg atttttcttt tactccgaac
      901 cgtggtggcg agagttaggg ttgacggatc acgtcgtaat tacccaaaat ccgatccgta
      961 aattgtattt caaagatgaa aaatacgcgt ttttctatac tgatagtgac aacgcgttat
     1021 attggcgaga tgtgctttct ttaggcgaag atcattacct tacaacggtt aaaaagctta
     1081 tcgcggaggc actaaactta cctcaagatg tcattccaaa tccagagtta cacaaaaaca
     1141 aattttggct tcatggtgtc gaattttcaa aagaatgttg ccctaaacat ccattttatc
     1201 aaacactttc tgatgacagc gttatctctt tgtctgacgc ctatacacac cattgtggat
     1261 ggatagaagg gggcatctta gccggcaagt ttacagcgga atcgatcatc agccagttgc
     1321 ctacacatcg tgaaactgtg aaagaagtag tatacctttg actcgagtct ggtaaagaaa
     1381 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta
     1441 acctaaactg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1501 gtcttgaggg gttttttgct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
     1561 tcactatttc ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
     1621 ttaagaagga gatatacata tgagtgtttt agattttcct cgaatacatt ttaagggtat
     1681 ggctcgggtt aatataccaa ccgcaaaccg aaatacccaa aacacattgg atacgacaac
     1741 aaataccgtg tttcaattcg gccagccggt tgacattgac ttgccgccgg cacgtttcca
     1801 cgaatacctc cactcacttg gtccaaaagt taatcgcttt ggtaaaaaag atgaaaatgg
     1861 agaattcagt gtagcgtgtg gctacaacat gcttggtaat aatcattttt catggcacga
     1921 cgctaaagtt acggctttgc aattgcgccc gggtgattgt gttgttcaag atccactcat
     1981 agggtgttct gttaatttat ggggacacta caatgagtac cttagaacct cattcaatcg
     2041 tgctcgctgg gttgataatg acccgacccg aagtgactcg gcactcatct atgctggaca
     2101 aattacatta actgcaaatg gcagtgaggc aagtgaaaag catgtgatgt ctgctgacat
     2161 tgattgtaca catggagtga gatggataaa tcctagcttt attaatagcc tcgacgaaca
     2221 tgcgtttgct gaagatattg cagcagcccg tttgtttcag tttgctgtca ataagaacag
     2281 caaggatttc ttattgaatc aattgggctt gcagtcagaa tttattgaac aattacgaat
     2341 agcactagaa caagaagatg ttaagggctt agttgttcaa tattgtgtaa gcaatctatc
     2401 gcctcctgta gcgcccaata tgccggtctt ttgtgattta catggctgca tagggctttg
     2461 gcgagaaagt gaaatggcca cttgtcctgc aggtcggatt ttacagccag aatgcccaca
     2521 gcaatttgca ccgattacat taaaacaggc agggcaatgg ttgagtataa atatgccgat
     2581 tagtatcggt catactcatc acttagatcc attacctgta ttatctggaa tgcctcctcg
     2641 attagcagca aaaacgccct tgggtgtctt gcaactcaag acggccagtg atgagttaat
     2701 cgccgagatc ccagaggtgc tttataacgc gtgtttggat acaggaggga tcatcgatgt
     2761 gacaatacaa acccatacag acggacttga attacagtca ttaaagctgg taagccagtc
     2821 tcattcgtgg catgaaatcg attggcaagt tcaagccgaa caacacatca tatatatgga
     2881 atctccaaac ctaatcgagt tgcaaaaaag caagcaatat attgatgtat atagctattt
     2941 tcgtggaatt cttaagccaa tcaacggttt aacaagttat aacgcgggac cacaacgagt
     3001 gcaatgtgac gagttcattg aaacgaatga acaaggacgt ggtttgtttc tagttgaagc
     3061 gttaacacca gggagtggtg aagtttttct aggagaacaa tatagtcgtg tccagatccg
     3121 tgtattaagt aatgactggg cgttacttaa tgtccctgat gaacaggttg actatgactt
     3181 tctttataaa aatgtcatgg cctactatga gtttgtttat ccgttcatgg cagacaaagt
     3241 gtttagcatg gctgataaat gtaaatgcga aacgtatgca cgcttaatgt ggcaaatgtg
     3301 cgatcccaaa aataaagaga aaagctacta catgcctagt acgcgggaga tgtcacaagc
     3361 acaatcgttt ctatttttga aatatttgag taacgtagaa aagtcggcaa tccccaaggt
     3421 tttgccttat tgcaatacag acgttacggt tacaggcaaa ataaaaaacc gtacacaatt
     3481 aattcgggca ttaaaagact ctgtcgacct agagttgtcg attatgctgc agtatttgta
     3541 tagcgcctat tcactaccaa attacgccgc cggagaggcg ttggtaaacg caaaaagatg
     3601 gactcaagaa caacttgaat tagtgaatgg tggcgcagat aggcggcaca acagcggttg
     3661 gcgaggtgct attcttgaag ttgctcacga agagatgatc cattatttgg tcgtcaataa
     3721 tttgcttatg tccttaggcg aaccttttta ctgtggcaac ccggtacttg gccaacaagc
     3781 gaacgaaaaa tttggactcg atacggattt ttcttacgag ccctttggct tacagtccat
     3841 cacgaaattt gtacgctttg agtggccacg ctttattccg accataggtc tttcaattgc
     3901 tgatttctat gccgacatta agcgtgcctt tgagcaatta ccagacctct atcctaaaga
     3961 tgcgaagggt tcggggggag agcatcactt gtttttgaat gaagtgatta accgtcaata
     4021 cccggcatac caattcgaag tgtataaccg acagactgct ttgtttgcaa ttgaatttgt
     4081 tacagagcaa ggcgaaggtg tgactgcaca gtcagaacaa ttcgaattag gtcatttcca
     4141 ccgcttaaga cagatagcca tggcattaac taatcaagaa cagccttttg aaccggcgta
     4201 tcccgtgctt aaaaaccctg tttttaaacc tagagcggga tgtaatttag tcaccgaccc
     4261 agaatcacaa gccttaatgg cgttttatca acagtgtcat gaattgatgt ttcaaatcat
     4321 gatgcatcat ttttcgcaac gacctctcat cagcatgcgg cgctcgcgat tgatgaatgc
     4381 cgccatcgac ctgatgacag gaatattacg tccattgtct gtgaaattaa tgacgatgcc
     4441 gtctgggact aaaggtcgaa atgccggtcc gccagtgcca gagtcggtca gttctttcgt
     4501 cgataaggat tatgctggcg gttgccaaca attgtcatgg cgctgcgcac agttggcggc
     4561 agcggcaaaa gcgttaaacc taaatgtaac tacacaaatc gaattattgg agtttttcca
     4621 acaacaactg gctgacctcg cagccggaac tctttcaaag gaaggttagc tcgagtctgg
     4681 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
     4741 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
     4801 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
     4861 aatacgactc actatttcgg aattgtgagc ggataacaat tcccctctag aaataatttt
     4921 gtttaacttt aagaaggaga tatacatatg gaattacgta aagtagatag agttccgcca
     4981 atgttacctg agtgttggag cagtagttat atttcttatt ggcttcctat gaagccagaa
     5041 gacgatatta cttctgggta ttgttggttc gattatgctc aagaggtttg tcgtatagac
     5101 ggtttgttta acccttggag tgaaacaaaa atgggacacc gtttgtggat gtccgaaatt
     5161 atgtatccac gcagttatga atcgtttaaa tctaaaattg cctatgcacg aacacatcgg
     5221 gataaacctt cagcgtttaa agaaacggtg ttaaatgatg aggtagaccc atgccatgag
     5281 cttattttaa cgcgtgatgt gttggtaaaa aaccacgcta agtatgcagg tgaacacacc
     5341 attttgggtt ataaagcgga tgcgtggtgc tttgaacgcc caaatgacaa aggtcctgca
     5401 acgtattttt ttaaagaagg gacagatcag ttgctccgaa tggtcacggg tgatccgaac
     5461 gttcacgcct ctgtaagaga ctttcctaat tttaatacgc tgccgatacc taacacgatt
     5521 tttgatgtaa caagtctctc tcataggaat tgactcgagt ctggtaaaga aaccgctgct
     5581 gcgaaatttg aacgccagca catggactcg tctactagtc gcagcttaat taacctaaac
     5641 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     5701 gggttttttg ctaggatcga gatcgatctc gatcccgcga aattaatacg actcactacc
     5761 cgggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     5821 gagatataca tatgagtaaa ataattattg ttggtggtgg gttggcaggc agcctgacgg
     5881 caatctatct agcgaaacaa gggcacgaag ttcacattat agaaaaacgt ggaaatcctt
     5941 tagaaagtca ggcggattat attgatcaag ttagcgcgcg tgcgattggt gtcagtatga
     6001 cagtacgagg aattgaagcc gttttagcag ccggtattcc cttggacgaa ttgcaggcgt
     6061 gcggcgttga agtttcaggt atggcgtttt ttatcaaagg tcaacctaaa atacgggatt
     6121 taccaccgtt agatacgtta aaaccgttgt cgttaagccg aagcatgttt caggtattgc
     6181 ttaacaagta cgctcataaa gcaaaagtac actatcacta tctacaacgc tgtgtcgacg
     6241 ttgatattga aagagcgtca gtgattacac aaaaccagta tggcgaattt gttaaccacc
     6301 aaggtgattt gctgattgct gcggatggcg cccgttcagt tgtccgagac gcatttcaaa
     6361 ctcatagtcg tcgttttgag tttcatcaat ccttttttaa gcatggttat aaaacactcg
     6421 tgatcccaga tgccacagaa gtcggtcttc gcaaagacat tttatatttc tttggtatgg
     6481 attctggagg ggaatttgca ggtcgtgcgg caactatccc tgatggcagt atcagttttg
     6541 ctgtgtgttt accttatacc ggaaaagtaa gtttacactc tgaagacacg caagcaatga
     6601 aggatttctt tgaacgctat tacgccatga tacctaagca cattcgagag tcgatgtacc
     6661 aacaatttat ggaaaagcca agtaatgact taattaatgt tcgatcgtca acctttcatt
     6721 ataaaaacag agcattgttg ttaggggatg cggcccatgc taccgcacct tttttaggac
     6781 agggaatgaa tatggcttta gaagacgcct ttgtccttga tagattgttt gccgagtttg
     6841 aaggaaactt aaccgaaatc ttgcctcgtt ttactgacct acgcaaaaaa gaagctgatg
     6901 cgatgcaaga tatggcgcgg gcaaactatg atgtcttgtc ttgctcaaat ccagtctttt
     6961 ttatgcgtgc gaaatttgcc cgttatatga caaaacaatt cccagatcgc tatccacccg
     7021 atatggctga aaaattatac tttacgtcta tggcgtatag cgagcttcgg gatgttcaac
     7081 aaaaacagaa tgtttggtac aaaataggga gaatgaatta actcgagtct ggtaaagaaa
     7141 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta
     7201 acctaaactg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     7261 gtcttgaggg gttttttgct agcgaaagga ggagtcgact atatccggat tggcgaatgg
     7321 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7381 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7441 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7501 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7561 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7621 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7681 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7741 aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat ggcatgagat
     7801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8761 tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8821 gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac gtgagttttc
     8881 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8941 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9001 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9061 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9601 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9661 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     9721 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
     9781 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     9841 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     9901 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     9961 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
    10021 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
    10081 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
    10141 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
    10201 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
    10261 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
    10321 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
    10381 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
    10441 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
    10501 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
    10561 gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc caccggaagg
    10621 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
    10681 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    10741 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
    10801 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
    10861 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
    10921 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
    10981 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
    11041 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
    11101 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
    11161 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
    11221 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
    11281 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
    11341 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
    11401 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
    11461 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
    11521 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
    11581 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
    11641 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
    11701 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
    11761 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
    11821 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
    11881 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
    11941 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
    12001 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
    12061 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
    12121 gatgccggcc acgatgcgtc cggcgtagcc taggatcgag atcgatctcg atcccgcgaa
    12181 attaatacga ctcactatt
//
