LOCUS       sequence-135339-       13599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Violacein pathway (vioABECD) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux 
            redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13599)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 13599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC-3A2-vioD
FEATURES             Location/Qualifiers
     source          1..13599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1374..1418
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1471..1518
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    1571..1595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1610..1632
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4682..4726
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4779..4826
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    4879..4903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4918..4940
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5566..5610
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      5663..5710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    5763..5787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5802..5824
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7134..7178
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      7231..7278
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    7331..7355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7370..7392
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8534..8578
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      8631..8678
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8722..9177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     9019..9040
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(9292..10083,10084..10152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9915..9934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10153..10245)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10326..10914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10815..10834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11068..11085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11100..11242
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11168..11187)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11328..11350
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11344..11535)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(12107..13189)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13151..13170
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13190..13267)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     13473..13492
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgttaaat ttagagcatt cagtttcaat tgtcggagct ggaatttctg
      121 ggatagtgtg tgcaattaca ttagctcgtt caagggcatt agaaggttac gcaatagatg
      181 tctatgaaca aagcccccgc gttggtggac ggatccaaac aaacttgttg agcaatcatt
      241 acatcgattt aggtgccgct cgtttttgtt cacaattgca tggtaatgta tatgatctag
      301 tcaagaggtt tgaactcgaa tttgaaccgt ttccttttac tcaacttaga tttactcagc
      361 caattcaaaa agaactcaag actattttgt ctaatctaaa accgttaatc gatgaacata
      421 aagaagattc ttttgtcgaa tttgttacga gctatttggg taaagaaaaa gcaaaagcag
      481 taatcttagc actgggctac gactcactct ctatgccaat aatttctcca catattgctt
      541 acgatatcat tgaaaaacac ccagaaactc aatgtttcac tgaaaattgt gggaatgaat
      601 ggtttagttt gaaagaggga ttggcgtcgt tgatcaaggc gctatatgaa gaggcgttaa
      661 aactcgggat taagtttcat tttgaatcta cattagttca aataagcaat cgaggtaacg
      721 cccacagctt ggtgattgcc gataatcaaa atcaacaaaa agagatcagt aaagggctta
      781 agatcattac tttgcctcca tctcgaatgg aaaaattaga taagcaattt cctaaacgtt
      841 ggagcaacta caattatggt tctagcccct tgtttaaagg atttttcttt tactccgaac
      901 cgtggtggcg agagttaggg ttgacggatc acgtcgtaat tacccaaaat ccgatccgta
      961 aattgtattt caaagatgaa aaatacgcgt ttttctatac tgatagtgac aacgcgttat
     1021 attggcgaga tgtgctttct ttaggcgaag atcattacct tacaacggtt aaaaagctta
     1081 tcgcggaggc actaaactta cctcaagatg tcattccaaa tccagagtta cacaaaaaca
     1141 aattttggct tcatggtgtc gaattttcaa aagaatgttg ccctaaacat ccattttatc
     1201 aaacactttc tgatgacagc gttatctctt tgtctgacgc ctatacacac cattgtggat
     1261 ggatagaagg gggcatctta gccggcaagt ttacagcgga atcgatcatc agccagttgc
     1321 ctacacatcg tgaaactgtg aaagaagtag tatacctttg actcgagtct ggtaaagaaa
     1381 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta
     1441 acctaaactg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1501 gtcttgaggg gttttttgct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
     1561 tcactatttc ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
     1621 ttaagaagga gatatacata tgagtgtttt agattttcct cgaatacatt ttaagggtat
     1681 ggctcgggtt aatataccaa ccgcaaaccg aaatacccaa aacacattgg atacgacaac
     1741 aaataccgtg tttcaattcg gccagccggt tgacattgac ttgccgccgg cacgtttcca
     1801 cgaatacctc cactcacttg gtccaaaagt taatcgcttt ggtaaaaaag atgaaaatgg
     1861 agaattcagt gtagcgtgtg gctacaacat gcttggtaat aatcattttt catggcacga
     1921 cgctaaagtt acggctttgc aattgcgccc gggtgattgt gttgttcaag atccactcat
     1981 agggtgttct gttaatttat ggggacacta caatgagtac cttagaacct cattcaatcg
     2041 tgctcgctgg gttgataatg acccgacccg aagtgactcg gcactcatct atgctggaca
     2101 aattacatta actgcaaatg gcagtgaggc aagtgaaaag catgtgatgt ctgctgacat
     2161 tgattgtaca catggagtga gatggataaa tcctagcttt attaatagcc tcgacgaaca
     2221 tgcgtttgct gaagatattg cagcagcccg tttgtttcag tttgctgtca ataagaacag
     2281 caaggatttc ttattgaatc aattgggctt gcagtcagaa tttattgaac aattacgaat
     2341 agcactagaa caagaagatg ttaagggctt agttgttcaa tattgtgtaa gcaatctatc
     2401 gcctcctgta gcgcccaata tgccggtctt ttgtgattta catggctgca tagggctttg
     2461 gcgagaaagt gaaatggcca cttgtcctgc aggtcggatt ttacagccag aatgcccaca
     2521 gcaatttgca ccgattacat taaaacaggc agggcaatgg ttgagtataa atatgccgat
     2581 tagtatcggt catactcatc acttagatcc attacctgta ttatctggaa tgcctcctcg
     2641 attagcagca aaaacgccct tgggtgtctt gcaactcaag acggccagtg atgagttaat
     2701 cgccgagatc ccagaggtgc tttataacgc gtgtttggat acaggaggga tcatcgatgt
     2761 gacaatacaa acccatacag acggacttga attacagtca ttaaagctgg taagccagtc
     2821 tcattcgtgg catgaaatcg attggcaagt tcaagccgaa caacacatca tatatatgga
     2881 atctccaaac ctaatcgagt tgcaaaaaag caagcaatat attgatgtat atagctattt
     2941 tcgtggaatt cttaagccaa tcaacggttt aacaagttat aacgcgggac cacaacgagt
     3001 gcaatgtgac gagttcattg aaacgaatga acaaggacgt ggtttgtttc tagttgaagc
     3061 gttaacacca gggagtggtg aagtttttct aggagaacaa tatagtcgtg tccagatccg
     3121 tgtattaagt aatgactggg cgttacttaa tgtccctgat gaacaggttg actatgactt
     3181 tctttataaa aatgtcatgg cctactatga gtttgtttat ccgttcatgg cagacaaagt
     3241 gtttagcatg gctgataaat gtaaatgcga aacgtatgca cgcttaatgt ggcaaatgtg
     3301 cgatcccaaa aataaagaga aaagctacta catgcctagt acgcgggaga tgtcacaagc
     3361 acaatcgttt ctatttttga aatatttgag taacgtagaa aagtcggcaa tccccaaggt
     3421 tttgccttat tgcaatacag acgttacggt tacaggcaaa ataaaaaacc gtacacaatt
     3481 aattcgggca ttaaaagact ctgtcgacct agagttgtcg attatgctgc agtatttgta
     3541 tagcgcctat tcactaccaa attacgccgc cggagaggcg ttggtaaacg caaaaagatg
     3601 gactcaagaa caacttgaat tagtgaatgg tggcgcagat aggcggcaca acagcggttg
     3661 gcgaggtgct attcttgaag ttgctcacga agagatgatc cattatttgg tcgtcaataa
     3721 tttgcttatg tccttaggcg aaccttttta ctgtggcaac ccggtacttg gccaacaagc
     3781 gaacgaaaaa tttggactcg atacggattt ttcttacgag ccctttggct tacagtccat
     3841 cacgaaattt gtacgctttg agtggccacg ctttattccg accataggtc tttcaattgc
     3901 tgatttctat gccgacatta agcgtgcctt tgagcaatta ccagacctct atcctaaaga
     3961 tgcgaagggt tcggggggag agcatcactt gtttttgaat gaagtgatta accgtcaata
     4021 cccggcatac caattcgaag tgtataaccg acagactgct ttgtttgcaa ttgaatttgt
     4081 tacagagcaa ggcgaaggtg tgactgcaca gtcagaacaa ttcgaattag gtcatttcca
     4141 ccgcttaaga cagatagcca tggcattaac taatcaagaa cagccttttg aaccggcgta
     4201 tcccgtgctt aaaaaccctg tttttaaacc tagagcggga tgtaatttag tcaccgaccc
     4261 agaatcacaa gccttaatgg cgttttatca acagtgtcat gaattgatgt ttcaaatcat
     4321 gatgcatcat ttttcgcaac gacctctcat cagcatgcgg cgctcgcgat tgatgaatgc
     4381 cgccatcgac ctgatgacag gaatattacg tccattgtct gtgaaattaa tgacgatgcc
     4441 gtctgggact aaaggtcgaa atgccggtcc gccagtgcca gagtcggtca gttctttcgt
     4501 cgataaggat tatgctggcg gttgccaaca attgtcatgg cgctgcgcac agttggcggc
     4561 agcggcaaaa gcgttaaacc taaatgtaac tacacaaatc gaattattgg agtttttcca
     4621 acaacaactg gctgacctcg cagccggaac tctttcaaag gaaggttagc tcgagtctgg
     4681 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
     4741 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
     4801 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
     4861 aatacgactc actatttcgg aattgtgagc ggataacaat tcccctctag aaataatttt
     4921 gtttaacttt aagaaggaga tatacatatg gaattacgta aagtagatag agttccgcca
     4981 atgttacctg agtgttggag cagtagttat atttcttatt ggcttcctat gaagccagaa
     5041 gacgatatta cttctgggta ttgttggttc gattatgctc aagaggtttg tcgtatagac
     5101 ggtttgttta acccttggag tgaaacaaaa atgggacacc gtttgtggat gtccgaaatt
     5161 atgtatccac gcagttatga atcgtttaaa tctaaaattg cctatgcacg aacacatcgg
     5221 gataaacctt cagcgtttaa agaaacggtg ttaaatgatg aggtagaccc atgccatgag
     5281 cttattttaa cgcgtgatgt gttggtaaaa aaccacgcta agtatgcagg tgaacacacc
     5341 attttgggtt ataaagcgga tgcgtggtgc tttgaacgcc caaatgacaa aggtcctgca
     5401 acgtattttt ttaaagaagg gacagatcag ttgctccgaa tggtcacggg tgatccgaac
     5461 gttcacgcct ctgtaagaga ctttcctaat tttaatacgc tgccgatacc taacacgatt
     5521 tttgatgtaa caagtctctc tcataggaat tgactcgagt ctggtaaaga aaccgctgct
     5581 gcgaaatttg aacgccagca catggactcg tctactagtc gcagcttaat taacctaaac
     5641 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     5701 gggttttttg ctaggatcga gatcgatctc gatcccgcga aattaatacg actcactacc
     5761 cgggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     5821 gagatataca tatgagtaaa ataattattg ttggtggtgg gttggcaggc agcctgacgg
     5881 caatctatct agcgaaacaa gggcacgaag ttcacattat agaaaaacgt ggaaatcctt
     5941 tagaaagtca ggcggattat attgatcaag ttagcgcgcg tgcgattggt gtcagtatga
     6001 cagtacgagg aattgaagcc gttttagcag ccggtattcc cttggacgaa ttgcaggcgt
     6061 gcggcgttga agtttcaggt atggcgtttt ttatcaaagg tcaacctaaa atacgggatt
     6121 taccaccgtt agatacgtta aaaccgttgt cgttaagccg aagcatgttt caggtattgc
     6181 ttaacaagta cgctcataaa gcaaaagtac actatcacta tctacaacgc tgtgtcgacg
     6241 ttgatattga aagagcgtca gtgattacac aaaaccagta tggcgaattt gttaaccacc
     6301 aaggtgattt gctgattgct gcggatggcg cccgttcagt tgtccgagac gcatttcaaa
     6361 ctcatagtcg tcgttttgag tttcatcaat ccttttttaa gcatggttat aaaacactcg
     6421 tgatcccaga tgccacagaa gtcggtcttc gcaaagacat tttatatttc tttggtatgg
     6481 attctggagg ggaatttgca ggtcgtgcgg caactatccc tgatggcagt atcagttttg
     6541 ctgtgtgttt accttatacc ggaaaagtaa gtttacactc tgaagacacg caagcaatga
     6601 aggatttctt tgaacgctat tacgccatga tacctaagca cattcgagag tcgatgtacc
     6661 aacaatttat ggaaaagcca agtaatgact taattaatgt tcgatcgtca acctttcatt
     6721 ataaaaacag agcattgttg ttaggggatg cggcccatgc taccgcacct tttttaggac
     6781 agggaatgaa tatggcttta gaagacgcct ttgtccttga tagattgttt gccgagtttg
     6841 aaggaaactt aaccgaaatc ttgcctcgtt ttactgacct acgcaaaaaa gaagctgatg
     6901 cgatgcaaga tatggcgcgg gcaaactatg atgtcttgtc ttgctcaaat ccagtctttt
     6961 ttatgcgtgc gaaatttgcc cgttatatga caaaacaatt cccagatcgc tatccacccg
     7021 atatggctga aaaattatac tttacgtcta tggcgtatag cgagcttcgg gatgttcaac
     7081 aaaaacagaa tgtttggtac aaaataggga gaatgaatta actcgagtct ggtaaagaaa
     7141 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta
     7201 acctaaactg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     7261 gtcttgaggg gttttttgct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
     7321 tcactatcat ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
     7381 ttaagaagga gatatacata tgaacatttt agtgatcggg gcaggtcctg ccggattaat
     7441 gtttgctagt caaattaaaa aactgaatcc agattgggat atttgtatta aagaaaagaa
     7501 taatcaagac gaaaccgtag gttggggcgt agtgctgcca gggcgcgcgc cacatcatcc
     7561 cgccaatcca ctttcgtatc ttcctaatta tgaatccttg gatgcacaat atttagatgc
     7621 ctttaaaatg actcaccaca atgaaagtgc gttaaaaagt acagggctaa ccttatgcgg
     7681 cgctgagcga aaatcgatga ttaaggctat gcgagatttg tgtattcgtt tgggtatcca
     7741 tattgattat gagcaaccag ttgaaaatct tgaattagct agcactcaat atgacttggt
     7801 tgtggtatca aacgggataa accacgccaa ccaattctat cgtgaagcgc taaaaccgaa
     7861 cattgaatat ggcacgaatc gctacatgtg gtatggcacg cctaatcttt ttaatgaaat
     7921 gaacctgatt tttaaagcgc attctaaagg tgtttttgtc gctcatgcct ataaatattc
     7981 gagtgacatg agtacttttg ttgttgaatg tagcgaagag acgtatctaa agtcaggtct
     8041 agacgaaatg tctgcagacc aagcagaagc gtttattgca tccgtatttg aatctgattt
     8101 gaacggaaag cctgttattg ctcaatcagg attgaaatgg cgtaatttca tgacgttaag
     8161 tcatgaaaag gcctatgatg gaaatattgt gttattgggc gatgcgttgc aatcgggtca
     8221 tttttcaatc gggcatggca cgaccatggc agtggttggc gcacagttac tgattaaatc
     8281 tgtccatcaa catgcaaata acgtcattgc tgtggtagag cactttaata cgcgagtgat
     8341 gcctgtcatg aaattgtttg atcaacatgc acaaacaagt cgcctttggt ttgaaagtgt
     8401 aggagagcta atgcatctaa gtacttctca aatcgctcaa agttttactg aacgtcgtga
     8461 gcagctgcct tctttaccag aagcgctagc aaaggcatta ggttatgcgc tgcaacgtta
     8521 actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc
     8581 tactagtcgc agcttaatta acctaaactg ctgccaccgc tgagcaataa ctagcataac
     8641 cccttggggc ctctaaacgg gtcttgaggg gttttttgct agcgaaagga ggagtcgact
     8701 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8761 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8821 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8881 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8941 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9001 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9061 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9121 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg
     9181 gcggcacgat ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10141 ttgaatactc atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc
    10201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa
    10261 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10321 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10381 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10441 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
    10501 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10561 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10621 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10681 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10741 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10801 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10861 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10921 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10981 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11041 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11101 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca
    11161 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
    11221 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
    11281 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
    11341 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
    11401 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
    11461 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
    11521 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
    11581 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
    11641 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
    11701 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
    11761 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
    11821 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
    11881 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
    11941 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat
    12001 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
    12061 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
    12121 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    12181 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
    12241 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
    12301 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
    12361 cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa
    12421 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
    12481 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
    12541 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
    12601 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
    12661 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
    12721 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
    12781 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
    12841 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
    12901 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
    12961 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
    13021 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
    13081 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
    13141 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
    13201 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
    13261 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
    13321 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
    13381 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
    13441 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
    13501 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagcc taggatcgag
    13561 atcgatctcg atcccgcgaa attaatacga ctcactatt
//
