LOCUS       sequence-135349-       13155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB236
FEATURES             Location/Qualifiers
     source          1..13155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2044..2733
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     protein_bind    2749..2873
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2910..2915,2916..2933,2934..2940)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2993..3652
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3059..3078)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3994..4299
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4178..4197
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4340..4464)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4496..4748
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4782..4798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4782..4798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4795..4817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4806..4822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4830..4836,4837..4854,4855..4860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4875..4896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4981..5153
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5210..5743)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(5806..6482)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5849..5869)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6677..6701
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7222..8013
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8259..8847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8748..8767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9001..9018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9033..9173
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9099..9118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9259..9281
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9517..9711
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9777..10850)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11279..11908)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttcaaa tgagtcacac acattgttga gagacatatc aaaataactc aattgtaagg
      361 taagttgata gatttcccag ttaaatgcat tattttaaca atatttgtat ctcaaatttt
      421 caaaatcata tcaaatttgc agatcttgat gatgattcac aactctgtct tcgatttttg
      481 attgttaacg ggtatcgata tttgggtaac ttaaaaagaa tttgctaaag atcttaaaaa
      541 acagattaaa aaaacctttc aaaatagcta atcctttaaa tccgtttcac aatataatgc
      601 tcattgatga taatcgaaaa gattccattt taaagtgcat ataaattgct taattatctc
      661 tttctgtctc gtaatatttt gttctctcct attggatcgg acccgcatct tctcgatcag
      721 ttcactatac taatatttgg tcaacttcaa gagagattag aacttcgttc tccaaagtgt
      781 aaaggccgac aagcagaaaa ggaaacttca atcagaagcc ttctaggttt tcccgcgtcc
      841 caaaaagcga agggaggacg gggaggacgg tgaggccaga agaagaaagc aacagtaaaa
      901 tctcatgtga ttgcgtgcat gtcatggatc tatgggatgc tagtccaccg tttggaagta
      961 tgtgcagaag gagaaaggcg cgggaggcca aaagcgactt cggccacctc tctactttct
     1021 agaaagtttg ctcgcagctt gtaataagcg cggaaaaggt actcgaaaaa ccggagcaat
     1081 cggtgtggta tcagcagtcg ggttcgctaa acctacgtct tcttattttg gaatgttaaa
     1141 ttgaaagggc gtgccggccc taagcacgga cgatgtgaca gacggaggag tgtgagcaat
     1201 tcggatgacg atgctggccg gcagtcacga caaagagtcc gaaggggcat ggacagggca
     1261 atatataaat ggaaaagagg ctttgaatat tcctaaccct tcgtcgtcgg cctcccaact
     1321 tgccctcgca tgctgaattg gccgcctatc tgcgaatggc cggccgccga ccctaagtcg
     1381 caataacttg aacggagccg atgcaaagtc cttatcgaac gctcgttatt ataaacgggg
     1441 cagacctagg tatttaatta tatcaaaacc ctccgcaatt tcgctaatca ttccctaatt
     1501 tcttctactt tggcgtctag tgctgtggtc gcgcttgctc tcaaagctcg ctgggaggca
     1561 gtggagcgtc tggcttaaaa tcgatcgccg caaccctaga gttcctttca cttctttctc
     1621 tcttgcgtgg agagcatcga catcgaagag ctgcatcttg tcttctggta tgcgccttgt
     1681 gccctcgtct ctgtacgtcg ttacgattgc tcgatattgt gattgtctag acctgaaacc
     1741 cgactggagc ttttacgtgg tgtcgattgg ggcctcgagc gagtgatttt ttaggttcgt
     1801 tttagccaag tacatcccac tttggtcagt cctgttatct cggtaaggtg ttcagttcgg
     1861 tctctggtgc ggaggtgcct cgtaagtttc ctgcatgata gatttgtcta tccttgactt
     1921 gaagaggagt cgaaggtcag cgaggttggc tgtgtgctat ggacgtcggt gctccttggt
     1981 tccgtgcttc tatgggtgtt gcctcaacag tattgatgtg cctgcagaaa ggttgagctt
     2041 acaatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     2101 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     2161 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     2221 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     2281 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     2341 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     2401 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     2461 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     2521 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     2581 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     2641 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     2701 agatactgcg acctccctag caaactgggg cacaagctag agttatcaac aagtttgtac
     2761 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     2821 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccc
     2881 ctcgaggggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2941 tgtggatttt gagttaggat ccgtcgagtt ttcaggagct aaggaagcta aaatggagaa
     3001 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     3061 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     3121 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     3181 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     3241 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     3301 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     3361 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     3421 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     3481 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     3541 gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag
     3601 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg
     3661 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     3721 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag
     3781 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     3841 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     3901 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     3961 gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa
     4021 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     4081 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     4141 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     4201 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     4261 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     4321 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     4381 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     4441 ctcagctttc ttgtacaaag tggttgataa cagcgcttag agctcgaatt tccccgatcg
     4501 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     4561 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     4621 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     4681 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     4741 actagatcgg gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg
     4801 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4861 gcctggggtg cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc
     4921 tgaattcaac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     4981 gatctggatt ttagtactgg attttggttt taggaattag attttattga tagaagtatt
     5041 ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta
     5101 taggaaccct aattccctta tctgggaact actcacacat tattatggag aaactcgagc
     5161 ttgtcgatcg actctaggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     5221 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     5281 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     5341 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     5401 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     5461 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     5521 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     5581 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     5641 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     5701 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtac catggtcgat cgacagatct
     5761 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     5821 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     5881 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     5941 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     6001 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     6061 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     6121 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     6181 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat
     6241 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     6301 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     6361 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     6421 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     6481 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     6541 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgaatt cagaaataaa
     6601 ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     6661 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     6721 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     6781 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     6841 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     6901 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg
     6961 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     7021 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     7081 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     7141 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     7201 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     7261 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     7321 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     7381 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     7441 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     7501 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     7561 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     7621 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     7681 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     7741 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     7801 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     7861 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     7921 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     7981 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     8041 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     8101 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     8161 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8221 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8281 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8341 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8401 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8461 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8521 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8581 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8641 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8701 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8761 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8821 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8881 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8941 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9001 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     9061 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     9121 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     9181 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     9241 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     9301 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     9361 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     9421 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     9481 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     9541 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     9601 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     9661 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
     9721 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     9781 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     9841 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
     9901 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
     9961 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
    10021 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
    10081 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
    10141 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
    10201 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
    10261 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
    10321 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
    10381 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
    10441 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
    10501 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
    10561 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
    10621 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
    10681 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
    10741 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
    10801 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
    10861 cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg
    10921 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
    10981 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
    11041 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
    11101 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
    11161 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
    11221 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
    11281 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
    11341 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
    11401 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
    11461 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
    11521 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
    11581 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
    11641 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
    11701 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
    11761 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
    11821 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
    11881 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
    11941 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
    12001 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
    12061 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
    12121 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
    12181 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
    12241 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
    12301 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
    12361 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
    12421 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
    12481 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
    12541 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
    12601 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
    12661 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
    12721 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
    12781 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
    12841 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
    12901 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
    12961 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
    13021 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
    13081 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
    13141 cgaacggata aacct
//
