LOCUS       sequence-135352-         556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB219
FEATURES             Location/Qualifiers
     source          1..556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..175)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(197..542)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(234..254)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gcataaagtg taaagcctgg ggtgcctaat gcggcccctc gaggggccgc catagtgact
       61 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      121 tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgttgata
      181 actctagagt cccccgtgtt ctctccaaat gaaatgaact tccttatata gaggaagggt
      241 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
      301 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
      361 ggggtccatc tttgggacca ctgtcggcag aggcatcttc aacgatggcc tttcctttat
      421 cgcaatgatg gcatttgtag gagccacctt ccttttccac tatcttcaca ataaagtgac
      481 agatagctgg gcaatggaat ccgaggaggt ttccggatat taccctttgt tgaaaagtct
      541 caattgccct ttggtc
//
