LOCUS       sequence-135365-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding 
            domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Garcia-Rodriguez C, Rao A
  TITLE     Requirement for integration of phorbol 12-myristate 13-acetate and 
            calcium pathways is preserved in the transactivation domain of 
            NFAT1.
  JOURNAL   Eur J Immunol. 2000 Aug . 30(8):2432-6.
  PUBMED    10940935
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2000 Aug . 30(8):2432-6."
COMMENT     Sequence Label: GAL4-mNFAT1(2-144)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    553..687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(590..609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     644..663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(697..716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(698..716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(803..820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttcgacgtcc cggagccgca gcccgacccc gatggcgggg acggccccgg ccacgagccc
       61 gggggcagtc cccaagacga gctggacttt tccatcctct tcgattatga ctatctgaac
      121 cctatcgaag aagaaccgat cgcacataag gccatcagct caccctccgg actcgcatac
      181 ccggatgatg tcctggacta tggcctcaag ccatgcaacc cccttgccag tccctctggc
      241 gagccccctg gccggttcgg agagccggat agtatagggt tccagaactt tctgagcccg
      301 gtcaagccag caggggcttc gggcccgagc cctcggatcg agatcactcc atcccacgaa
      361 ctgatgcagg cagggggggc cctccgtggg agagacgccg gcctgtcccc cgagcagcag
      421 gccctggccc tgtctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt
      481 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      541 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      601 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      661 atcaatgtat cttatcatgt ctggatctgc cggtctccct atagtgagtc gtattaattt
      721 cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      901 acgca
//
