LOCUS       sequence-135367-        3852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding 
            domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Garcia-Rodriguez C, Rao A
  TITLE     Requirement for integration of phorbol 12-myristate 13-acetate and 
            calcium pathways is preserved in the transactivation domain of 
            NFAT1.
  JOURNAL   Eur J Immunol. 2000 Aug . 30(8):2432-6.
  PUBMED    10940935
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2000 Aug . 30(8):2432-6."
COMMENT     Sequence Label: GAL4-mNFAT1(2-144)
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     20..37
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(48..68)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             382..822
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    1381..1515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1418..1437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1472..1491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1525..1544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1526..1544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1631..1648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1802..2390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1882..1901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2561..3352,3353..3421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3184..3203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3422..3526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3594..3612
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3650..3672)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3772..3791
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgtatcat acacatacga tttaggtgac actatagaac tcgatgtgga atgtgtgtca
       61 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      121 caattagtca gcaaggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
      241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
      301 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aagaggcttt tttggaggag
      361 atctaagctg cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt
      421 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag
      481 aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat
      541 ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct
      601 cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta
      661 acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca
      721 gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg
      781 gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccggaatt cgacgtcccg
      841 gagccgcagc ccgaccccga tggcggggac ggccccggcc acgagcccgg gggcagtccc
      901 caagacgagc tggacttttc catcctcttc gattatgact atctgaaccc tatcgaagaa
      961 gaaccgatcg cacataaggc catcagctca ccctccggac tcgcataccc ggatgatgtc
     1021 ctggactatg gcctcaagcc atgcaacccc cttgccagtc cctctggcga gccccctggc
     1081 cggttcggag agccggatag tatagggttc cagaactttc tgagcccggt caagccagca
     1141 ggggcttcgg gcccgagccc tcggatcgag atcactccat cccacgaact gatgcaggca
     1201 gggggggccc tccgtgggag agacgccggc ctgtcccccg agcagcaggc cctggccctg
     1261 tctagataag taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta
     1321 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     1381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     1501 tatcatgtct ggatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg
     1561 ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1681 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2221 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2521 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2581 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2641 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2701 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2761 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2821 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2881 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2941 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3001 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3061 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3121 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3181 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3241 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3301 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3361 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3421 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3481 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3541 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3601 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3661 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3721 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     3781 agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc ggctacaatt
     3841 aatacataac ct
//
