LOCUS       sequence-135381-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C16orf80 in mammalian cells with FLAG-tag epitope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pMXs-C16orf80-flag
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             654..719
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tggaccatcc tctagactgc cggatctagc tagttaatta agccaccatg ttcaaaaaca
       61 cgttccagag cggcttcctc tccatcctct acagcatcgg cagcaagcct ctgcaaatct
      121 gggacaaaaa ggtacggaat ggccacatca aaagaatcac tgataatgac atccagtccc
      181 tggtgctaga gattgaaggg acaaatgtaa gcaccacata tatcacatgc cctgcagacc
      241 ccaagaagac gctgggaatt aaacttcctt tccttgtcat gattatcaaa aacctgaaga
      301 agtattttac cttcgaagtg caggtactag atgacaagaa tgtgcgtcgt cgctttcggg
      361 caagtaacta ccagagcacc acccgggtca aacccttcat ctgcaccatg cccatgcggc
      421 tggatgacgg ctggaaccag attcagttca acttgctaga cttcacacgg cgagcatacg
      481 gcaccaatta catcgagacc ctcagagtgc agatccatgc aaattgtcgc atccgacggg
      541 tttacttctc agacagactc tactcagaag atgagctgcc ggcagagttc aaactgtatc
      601 tcccagttca gaacaaggca aagcaagcag gaggcggtgg aggcggtgca ggcgactaca
      661 aagaccatga cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagt
      721 aataataaat tccgcccccc cccc
//
