LOCUS       sequence-135392-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB212
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(79..85,86..103,104..109))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..270)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(296..313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(296..312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(305..327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    515..539
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
       61 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
      121 ctaatgcggc ccctcgaggg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca gcttttttgt acaaacttgt tgataactct agacccaagc ttggcactgg
      301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      421 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
      481 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
      541 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
      601 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
      661 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
      721 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctt
//
