LOCUS       sequence-135394-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB319
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..163)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(185..530)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(222..242)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 aagcctgggg tgcctaatgc ggcccctcga ggggccgcca tagtgactgg atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgttgataac tctagagtcc
      181 cccgtgttct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
      241 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
      301 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
      361 tgggaccact gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc
      421 atttgtagga gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc
      481 aatggaatcc gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt
      541 ggtcttctga gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca
      601 tgttgacgaa gattttcttc ttgtcattga gtcgtaagag actctgtatg aactgttcgc
      661 cagtctttac ggcgagttct gttaggtcct ctatttgaat ctttgactcc atggcctttg
      721 attcagtggg aactaccttt ttagagactc c
//
