LOCUS       sequence-135395-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB102
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(82..88,89..106,107..112))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(149..273)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(295..640)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(332..352)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg
       61 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
      121 tgcctaatgc ggcccctcga ggggccgcca tagtgactgg atatgttgtg ttttacagta
      181 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      241 cgtttctcgt tcagcttttt tgtacaaact tgttgataac tctagagtcc cccgtgttct
      301 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
      361 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
      421 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
      481 gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga
      541 gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc
      601 gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt ggtcttctga
      661 gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca tgttgacgaa
      721 gattt
//
