LOCUS       sequence-135410-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Sema3A protein (truncated at cleavage site P3; ie, 
            long and missing exon 3), C-terminally fused to alkaline phosphatase
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3a(L, del3)-AP-His
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      157..166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgttgcggtg ctgttaacgg tggagggcag tgtagtctga gcagtactcg ttgctgccgc
       61 gcgcgccacc agacataata gctgacagac taacagactg ttcctttcca tgggtctttt
      121 ctgcagaagc ttgaattctc gaggatccgc ggccgcgcca ccatgggctg gttcactggg
      181 attgcctgtc ttttctgggg tgtattactt acagccagag caaactatgc aaacggaaag
      241 aacaatgtgc caagactgaa attatcgtac aaagaaatgt tggaatccaa caatgtgatc
      301 acttttaatg gcttggccaa cagctccagt taccacacct tccttctgga tgaagaacgg
      361 agtagactat atgttggagc aaaagatcat atattttcat tcaacttggt gaacattaaa
      421 gattttcaaa agattgtgtg gccagtatct tacacaagga gagatgaatg caaatgggct
      481 ggaaaagata tcctgaaaga atgtgccaat ttcatcaagg tcctggaggc ttataatcag
      541 actcacttgt atgcctgtgg aactggggct ttccatccaa tctgcaccta tattgaagtt
      601 ggacatcatc ctgaggacaa catttttaag ctgcaggact cacattttga aaacggtcgt
      661 gggaagagcc cttatgatcc caaactactg actgcctctc ttctaataga cggtgagttg
      721 tactctggaa ctgctgcgga cttcatggga cgggacttcg ctatcttcag aacactg
//
