LOCUS       sequence-135419-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Expression Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: pcw107
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(87..93,94..111,112..117))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(142..266)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(350..368)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
       61 cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc
      121 tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
      181 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
      241 cgttcagctt ttttgtacaa acttgttgat ccatcgatgc tagcccctgg ggagagaggt
      301 cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc ttgcagaatg
      361 cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc gcctcccgca
      421 ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta ttggccacag
      481 cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg agggtactag
      541 tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag gaaccttccc
      601 gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg gcgaagatcc
      661 gggtgacgct gcgaacggac gtgaa
//
