LOCUS       sequence-135420-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Expression Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: pcw107
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..235)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(320..340)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(738..749)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      121 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      181 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttaccg
      241 gttagtaatg aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
      301 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
      361 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
      421 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
      481 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
      541 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
      601 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
      661 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
      721 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
      781 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacga
//
