LOCUS       sequence-135427-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses DREADDs Gq receptor under oxytocin promoter control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Penagarikano O, Lazaro MT, Lu XH, Gordon A, Dong H, Lam HA, Peles E,
            Maidment NT, Murphy NP, Yang XW, Golshani P, Geschwind DH
  TITLE     Exogenous and evoked oxytocin restores social behavior in the 
            Cntnap2 mouse model of autism.
  JOURNAL   Sci Transl Med. 2015 Jan 21;7(271):271ra8. doi: 
            10.1126/scitranslmed.3010257.
  PUBMED    25609168
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2015-01-21"; volume: "7(271):271ra8. doi"; pages: " 
            10.1126/scitranslmed.3010257"
COMMENT     Sequence Label: AAV-mOXT-hM3Dq-mCherry-WPRE
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..72)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(81..791)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(359..378)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     628..646
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      788..797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat agcatacatt
       61 atacgaagtt atggcgcgcc ttacttgtac agctcgtcca tgccgccggt ggagtggcgg
      121 ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
      181 aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg
      241 gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg
      301 cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc
      361 ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag
      421 atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg
      481 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg
      541 atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg
      601 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc
      661 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
      721 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
      781 ttgctcacca tggtggcgac cgg
//
