LOCUS       sequence-135429-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pBAC-lacZ
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(56..61,62..79,80..86)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     96..118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     115..131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     115..131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(168..185)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(168..184)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(177..199)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgcgatcgcg atgaccgtag gcgcgccggt ctctattaca ttaggcaccc cgggctttac
       61 actttatgct tccggctcgt atgttgtgtc gaccgagcgg ataacaattt cacacaggaa
      121 acagctatga ccatgattac gaatttcgac ctgcagccaa gcttggcact ggccgtcgtt
      181 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      241 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      301 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
      361 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
      421 cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat
      481 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
      541 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
      601 catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa
      661 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc
      721 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
      781 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
      841 tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
      901 cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
      961 accgcgcctt tcggcggtga aattatcgat ga
//
