LOCUS       sequence-135430-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pBAC-lacZ
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    494..527
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    545..943
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
ORIGIN
        1 tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc
       61 gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt
      121 gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg
      181 cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta
      241 cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac
      301 atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc
      361 agtatcggcg gaattacagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca
      421 aaaataatta attaactccg gatctagagc ccgcctaatg agcgggcttt tttttcttaa
      481 ggccgcatcg aatataactt cgtataatgt atgctatacg aagttattag cgatgagctc
      541 ggacttccat tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa
      601 gctcgctttc agcacctgtc gtttcctttc ttttcagagg gtattttaaa taaaaacatt
      661 aagttatgac gaagaagaac ggaaacgcct taaaccggaa aattttcata aatagcgaaa
      721 acccgcgagg tcgccgcccc gtaacctgtc ggatcaccgg aaaggacccg taaagtgata
      781 atgattatca tctacatatc acaacgtgcg tggaggccat caaaccacgt caaataatca
      841 attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag
      901 acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtccga
//
