LOCUS       sequence-135433-         512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant human codon-optimized dCas9-DNMT3A fusion 
            (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-EGFP (ANV)
FEATURES             Location/Qualifiers
     source          1..512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(302..322)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(329..394)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      394..403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccaccttggc catctcgttg ctgaagatct cttgcagata gcagatccgg ttcttccgtc
       61 tggtgtatct tcttctggcg gttctcttca gccgggtggc ctcggctgtt tcgccgctgt
      121 cgaacagcag ggctccgatc aggttcttct tgatgctgtg ccggtcggtg ttgcccagca
      181 ccttgaattt cttgctgggc accttgtact cgtcggtgat cacggcccag cccacagagt
      241 tggtgccgat ggccaggccg atgctgtact tcttgtcggc tgctgggact ccgtggatac
      301 cgaccttccg cttcttcttt ggggccatct tatcgtcatc gtctttgtaa tcaatatcat
      361 gatccttgta gtctccgtcg tggtccttat agtccatggt ggcaccggtc caacctgaaa
      421 aaaagtgatt tcaggcaggt gctccaggta attaaacatt aataccccac caaccaacca
      481 tcccttaaac ccttacctct tgctcagcta at
//
