LOCUS       sequence-135435-         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting 
            control sgRNA for use in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_hNT
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(309..329)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(336..401)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      401..410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgtcgtcca ccttggccat ctcgttgctg aagatctctt gcagatagca gatccggttc
       61 ttccgtctgg tgtatcttct tctggcggtt ctcttcagcc gggtggcctc ggctgtttcg
      121 ccgctgtcga acagcagggc tccgatcagg ttcttcttga tgctgtgccg gtcggtgttg
      181 cccagcacct tgaatttctt gctgggcacc ttgtactcgt cggtgatcac ggcccagccc
      241 acagagttgg tgccgatggc caggccgatg ctgtacttct tgtcggctgc tgggactccg
      301 tggataccga ccttccgctt cttctttggg gccatcttat cgtcatcgtc tttgtaatca
      361 atatcatgat ccttgtagtc tccgtcgtgg tccttatagt ccatggtggc accggtccaa
      421 cctgaaaaaa agtgatttca ggcaggtgct ccaggtaatt aaacattaat accccaccaa
      481 ccaaccatcc cttaaaccct
//
