LOCUS       sequence-135436-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting 
            control sgRNA for use in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_hNT
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccgcctctc tcggccttgg tcagattgtc gaactttctc tgggtaatca gcttggcgtt
       61 cagcagctgc cgccagtagt tcttcatctt cttcacgacc tcttcggagg gcacgttgtc
      121 gctcttgccc cggttcttgt cgcttctggt cagcaccttg ttgtcgatgg agtcgtcctt
      181 cagaaagctc tgaggcacga tagcgtccac atcgtagtcg gacagccggt tgatgtccag
      241 ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca gcttctcgtt
      301 ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca gctctttgat
      361 gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct gggtggtctg
      421 gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca tcactttcac
      481 gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg ggctgccggc
      541 cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg ctttctggat
      601 gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc tgttggcgaa
      661 gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc ggatgccgtt
      721 gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca gctgcttcat
      781 cactttgtcg tcgaa
//
