LOCUS       sequence-135446-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human TGIF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_TGIF2
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(887..903)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(896..918)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
       61 gcatcgcctt ctatcgcctt cttgacgagt tcttctgatt cgaacatcga agggtgctga
      121 aggctccagc cagctgtcct gggtttccgt tttggttccc tttcatacag agggttttct
      181 atggatcact gccaaacatt gggatcatct cctctgtcca gaggtcttca acaggaagat
      241 gccagctggc accactgcac tgtgatgggg gccctctcct ctgctgactc tgccgtttct
      301 ccaggcctcc gctcagtgat gagaccaaga gatcggagac aagcatggtg ctgctgcttc
      361 tgctgcttct ccagaaaatc cctgggacac ctttgttcca gcctggtttc ctgggctggg
      421 ctcaggaaag ctgccaaatt cagtcctatg ttgggtccaa gctgcccctg tgctgtttct
      481 gtcaagccag gtgtggacat tccaagttca tatgcgtgaa caaaagaaaa gaggaaccca
      541 gtggatgtaa cagaaccgac tccagttgaa tgtttagatt tttgctaaac tgttttcttt
      601 ttcccttttt tgctgtggtt tgcattcacg gcagtagtta gcccaggtgt ggggaacgag
      661 agtgcactgc atgatagcgt tctggtgagc tgggaaggac ccaccactgc cactgaggat
      721 tgttttggaa gaaaggaata tttttatctt ggggaccagc taagtctctg cagtagtgtg
      781 aaattccaaa tggttgtttt atcattggtt tggtttacca aaaaaaaggc agggcttgga
      841 aagtcctctc cactgactgt agcctccaat tcactggccg tcgttcactg gccgtcgttt
      901 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      961 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1021 tgcgcagcct gaatggcgaa tgcgatttat tcaacaaagc cgccgtcccg tcaagtcagc
     1081 gtaatg
//
