LOCUS       sequence-135456-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains 600 bp homology arms to SS9 for one-step markerless genome 
            integration in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, 
            Mutalik VK, Arkin AP, Gill RT
  TITLE     Rapid and Efficient One-Step Metabolic Pathway Integration in E. 
            coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 22.
  PUBMED    27072506
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 22."
COMMENT     Sequence Label: pSS9
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctttactca ttttttctac cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
       61 aagcttggcg taatcatggt catggtatat ctccttcttg ggaaaccgtt gtggtctccc
      121 tggctgtaag tatcctatag gttagacttt aagtcaatac tctttttgat aaacctgttt
      181 gacatatcaa ctgcgccaga ggtaggattg aaaacgctct cctgattttc caattcattt
      241 tctggataaa taaataattt atttttgtca ctattattta tgtaatcatc ctgtcaggga
      301 gagggatctc aattatcaat gcttaattac gtcatcattt tgatgacatg aaaccggagc
      361 attttgcatg gaattttcgt tttcacccaa acgtcttgtt gttgctgtcg ccgccgctct
      421 tcctctcatg gccagcgcag cagatacccc gtcaactgcc accgcacgca aaggctttgc
      481 cggatacgat cacccaaacc agtatctggt taaaccggcg accactattg
//
