LOCUS       sequence-135461-       10127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinB2 protein (ie, 
            long), C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb2(L)-AP-His
FEATURES             Location/Qualifiers
     source          1..10127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..3097
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6707..6724
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6750..6871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6787..6806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6841..6860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6909..6926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7080..7668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7160..7179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7839..8630,8631..8699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8462..8481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8700..8804)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8872..8890
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8928..8950)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8952..8972)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8957..9287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9138..9273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9424..9443
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9510..9965)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9647..9668)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9859..9878
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10091..10113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10105..10122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10106..10122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggctctgcc
     1621 actctgggcc ctgacctttc taggtctcac gggcctaggg ttgagcctgc ggtcccgaaa
     1681 gccggagagt ttccgcagtg agacagagct gaaccacctg gctgtggatg aggtcacagg
     1741 tgtggtgtat gtcggggcag tgaacgcgct ctaccagctg agtgccgacc tgcacgtcca
     1801 gcagcacgtg gtcacaggcc ccttcatgga taacaagaag tgcacaccac ccattgaggc
     1861 cagccagtgc cacgaagcgg tgctcactga caacttcaac cagctgctgc tgctcgaccc
     1921 accggggaaa cgcctggtgg agtgcggcag cctcttcaag ggcatctgtg ccctgcgcgc
     1981 catgagcaac atctcggtgc gcctcttcta cgaggatggc agcggcgaga agtccttcgt
     2041 ggccagcaat gacgagagag tggccaccgt ggggctggtg acctccacgc gccccgacgg
     2101 cgagcgcgtg ctgtttgtgg gcaaaggcaa tgggccgcac gacaatggta tcatcgtgag
     2161 cacccgcctg ctggacaggg ctgagggccg ggaggccttt gaggcctact ctgaccacac
     2221 caccttcaag gccagctacc tgtccacgaa tactcagcag tttgtcgcag cctttgagga
     2281 tgatttctat gtcttcttcg tctttaacca tcaagacaag catcctgcta agaaccgcac
     2341 gctgctggcg cgcatgtgca aagacgaccc ctcctactat tcctatgtag agatggacct
     2401 gcagtgtcag gatcccagtg acccccaaga ctctgccttt ggcacctgcc tagccgcttc
     2461 tgtagccaca tcgggtgctg gcagagcact ttatgctgtc ttcagcagag atggccggag
     2521 cactggggga cctggcgcgg gcctctgtgt gttcccgcta gataaagtcc gtgagaagat
     2581 agaagccaac cgtaacgcct gctacacagg tgcccgagag gcaggccgca ctatcttcta
     2641 caagcccttc cacggagaaa tccagtgtgg cggccatctg ataggtgcca gcgagagctt
     2701 cccatgcggc tcggagcatc tgccctatcc actgggcagt cgtgatggac tcgtcgccac
     2761 agccgtgctg caccgcgggg gcctgaatct gacagcagtg acagtaactg ccgagaatga
     2821 ccacactgtt gcgttcctgg gcacctcaga tggccggatc cttaaggtgt accttgctcc
     2881 agacggcact tctgcagagt atggttctat cccagtagac atcaataaga aaatcaagca
     2941 ggacctggca ctgtctggaa acctgtccag tctgtatgct atgacccagg acaaggtgtt
     3001 ccggctccca gtacaagaat gcctgagcta tgtaacctgc gctcagtgtc gtgactccca
     3061 agatccctac tgtggctggt gtgtcatcga gggacgatgc accaggaagt ccgagtgctc
     3121 acgggccgag gaaaccggac actggctgtg gagccgggag aagtcctgtg tggccatcac
     3181 tgatgccttt ccacagaaca tgagccggcg ggcccaagga gaggtacgcc tgtctgtcag
     3241 ccccctgccc accctgactg aggacgatga gttactgtgc ctctttggtg actcaccacc
     3301 ccaccctgcc cgggtagagg acgataccgt catctgtaat tccccaagca gcatccctag
     3361 tacaccgcca ggccaagacc atgttgacgt gagcatccag ctcctcttaa aaagcggcag
     3421 tgtcttcctc acctcccacc agtatccctt ctatgactgc cgtgaggcca tgagcctggt
     3481 ggagaacctg ccgtgcatct cttgtgctag caaccgctgg acttgccaat gggacctgca
     3541 gtactacgag tgtcgggagg cttcgcccaa cccagaggag ggaatcatac gtgcccacat
     3601 ggaggacaac tgcccccagt tcctggcccc tgaccctttg gtcatcccca tgaaccatga
     3661 gacagaggtg accttccagg gcaagaactt ggagactgtg aaggtttctt ccctgtatgt
     3721 gggcagtgag ctgctgaatt ttgaagagac tgtgaccatg catgaatcag acaccttctc
     3781 ttttaggacc ccaaagctat cccatgatgg taatgagaca ctgcctcttc acctgtatgt
     3841 taagtccttt ggcaagaaca ttgacagcaa gctacaagtg actctctaca actgctcctt
     3901 tggccgcagt gactgtagcc tgtgtctggc tgccgatcct gcctacaggt gtgtgtggtg
     3961 ccgtgggcag aaccggtgcg tgtacgaagc tctgtgcagc aatgtcactt ctgagtgccc
     4021 accaccagtt atcactagga tccagcctga gacgggcccg ctgggtgggg gcatcctggt
     4081 cactatccat gggtccaatc tgggtgtcaa agcagatgac gtcaagaaga taactgtggc
     4141 tggccagaac tgtgcctttg aaccaagagg gtactccgta tccacccgga ttgtgtgtgc
     4201 aattgaggct tcggagatgc ccttcacagg aggcattgag gtggatgtta atggaaaact
     4261 cggccattca ccgccacacg tccagttcac ttatcaacaa ccccagcctc tcagtgtgga
     4321 gccacgacag gggccacagg caggtggcac cacattgacc atcaatggca ctcacctgga
     4381 cacaggctcc aaggaggatg tgcgggtgac actcaatgac gtcccttgtg aagtgacaaa
     4441 gtttggagca cagctacagt gtgtcacagg tcaacagttg gctccaggcc aggtgacact
     4501 agaaatctac tatgggggct ccagagtgcc cagccccggc atctctttca cctactgcga
     4561 gaaccccatg atacgagcct ttgagccatt gagaagcttt gtcagtggtg gccggagcat
     4621 caacgttact ggccagggct tcagcctcat ccagaagttt gccatggttg tcatcgctga
     4681 gcccttgcgg tcctggaggc ggcggcggcg ggaggctgga gccctggagc gtgtgacggt
     4741 cgagggcatg gagtacgtgt tctacaacga caccaaggtc gtcttcttgt ctcctgctgt
     4801 ccccgaagag cccgaggctt acaacctcac cgtgctgata cggatggacg gtcactgtgc
     4861 cccgctcagg accgaagccg gagtctttga gtatgtggca gaccccacct ttgagaactt
     4921 cacgggtggc gtcaaaaagc aggtcaacaa gctcatccac gcccggggaa ccaatctgaa
     4981 caaagccatg actctggagg aggcagaggc atttgtgggt gctgagcgtt gcattatgaa
     5041 gacgctgacc gagacggacc tgtactgcga gcccccagag gtgcagcctc cacccaagcg
     5101 gcggcagaag cgagacacgg cacacaacct acctgagttc attgtgaagt ttggctcccg
     5161 agagtgggtg ctaggccgag tggagtacga cacgcgtgcg agtgacgtgc cgctcagtgg
     5221 cgcgcctgga tcttccggaa tcatcccagt tgaggaggag aacccggact tctggaaccg
     5281 cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa
     5341 gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat
     5401 cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt
     5461 cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc
     5521 cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc
     5581 agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa
     5641 tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc
     5701 ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt
     5761 gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat
     5821 ggacattgac gtgatcctag gtgggggccg aaagtacatg tttcgcatgg gaaccccaga
     5881 ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt
     5941 gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat
     6001 gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat
     6061 gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc
     6121 tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg
     6181 catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt
     6241 cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt
     6301 cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat
     6361 cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg
     6421 aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag
     6481 cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg
     6541 cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga
     6601 gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg
     6661 cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggtcatc atcaccatca
     6721 ccattaatct agagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
     6781 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6841 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta gctagactct
     6901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8821 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8881 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8941 cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     9001 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     9061 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     9121 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9181 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     9241 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     9301 cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9361 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9421 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9481 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     9541 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     9601 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     9661 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     9721 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     9781 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     9841 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     9901 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     9961 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    10021 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    10081 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
