LOCUS       sequence-135462-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Netrin G1, isoform j 
            protein (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to alkaline phosphatase + 
            6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Ntng1.j-AP-His
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agatctctag attaatggtg atggtgatga tgacccgggt gcgcggcgtc ggtggtgccg
       61 gcggggggcg ccaggtcgca ggcggtgtag ggctccaggc aggcggcgaa ggccatgacg
      121 tgcgctatga aggtctgctc ctgcacgccg tgaaccaggt gcgcctgcgg gccgcgcgcg
      181 aacaccgcca cgtcctcgcc tgcgtgggtc tcttcgtcca ggggcactgc tgactgctgc
      241 cgatactcgg ggctcccgct ctcgctctcg gtaacatccg gccgggcgcc gtccttgagc
      301 acatagcctg gaccgtttcc gtataggagg accgtgtagg ccttcctgtc ccgggccttg
      361 ccaggggcca gcccgaagat ggagctccct cgcagggggt agcctccgaa ggagaagacg
      421 tgggagtggt cggcagtgac gaggctcagc gtgtcctcct cgctggtgag ctggcccgcc
      481 ctctcaatgg cgtcgtcgaa catgatcgtc tcagtcagtg cccggtaagc cctgctttca
      541 tgatgaccat ggtcgatgcg accaccctcc acgaagagga agaagccgcg ggggttcctg
      601 ctcagcaggc gcagggcagc ctctgtcatc tccatcaggg aggggtccag tgtggagtct
      661 cggtggatct cgtatttcat gtctccaggc tcaaagagac ccatgagatg ggtcacagac
      721 gggtccaggg aagcctgcat gagctcagtg cggttccaca cataccgggc accctggcgc
      781 ttcgccagcc attcctgcac cagattcttc c
//
