LOCUS       sequence-135466-       10244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinB3 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb3-AP-His
FEATURES             Location/Qualifiers
     source          1..10244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6824..6841
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6867..6988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6904..6923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6958..6977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7026..7043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7197..7785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7277..7296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7956..8747,8748..8816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8579..8598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8817..8921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8989..9007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9045..9067)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9069..9089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9074..9404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9255..9390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9317..9336
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9541..9560
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9627..10082)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9764..9785)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9976..9995
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10208..10230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10222..10239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10223..10239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgctgactga
     1621 cttcctccag gctcctgtga tggctccctg gtctcccttc agcctccacc tgctgctgct
     1681 gttcctgccg ctgctgcctt tgaccagggt ccatcgcttc tctgtaccaa ataccagctt
     1741 caaccacctg gtgctggcac cagatcaggg caaactctac gtgggtgcag tgaatcacct
     1801 ctttcagctc agccctgagc tgaagatgga gtctgtggct gtcactggcc ctgtcattga
     1861 cagtcctgat tgtgtgcctt tccgtgacct ggctgagtgc ccacaggccc agctcactga
     1921 caatgccaac cagctcttgt tggtgagcag caggacacag gaactggtgg cttgtgggca
     1981 agtgaagcag ggtgtatgtg agaagcggcg ccttggggat gtgactcaag tgttgtacca
     2041 ggctgaggac cctggtgatg ggcagtttgt ggctgccaat accctgggag tgaccacagt
     2101 aggcctagtg gtgcccttgc caggccgaga cctcctgcta gtggccagag gcctggcagg
     2161 caagctgtca gcaggagtgc cacccctaac tgtgcgccag ctggctgggc cacagccttt
     2221 ctccagtgaa ggactgggaa gacttgtggt cggtgacttt tcagactaca acaatagcta
     2281 tgtaggagct ttttctgatg ctcactctgc ctactttgtg ttccgtcgcc gtggggctcg
     2341 ggcccagact gagtatcgct cctatgtagc tcgtgtctgc ctcagagatg tcaaccttta
     2401 ttcctatgtg gaaatgccac ttacctgcca tggccagggc cttatccagg ctgccttcct
     2461 tactccagac actttgctag gtgcattttc agcaggcaca agtcaggcac aggcggccct
     2521 gtgtgccttt cccttagctg acctggacag gagtatggaa caagccaggc gactttgcta
     2581 tactactggt ggtcagggtc ctagcggcat ggaagaagcc actgtggagt atggtgtcac
     2641 atcccgttgc gtcactttgc ctcctgactc ccccgagtca tacccctgtg gtgatgaaca
     2701 cacccccagc cccatcgcag gccgccaacc cctggaggcc cagcctctgc tgcaactggg
     2761 acagtccatc agtgcagtgg ctgctctcca gacagatggg cacacgatag ccttcctggg
     2821 agacacccaa ggccagctac ataaggtctt tctaaacagc tcccatggcc aggtatacca
     2881 ttcccagcaa gtggggcccc caggctcagc catcagtcca gatctgctgg tagacagcaa
     2941 tggagaccac ctctatgtct tgactgccca gcaggtagac cggatacttg tggcagcctg
     3001 tcctcagttt cctaactgca ctacctgtct ccaggccagg gacccactat gtggttggtg
     3061 catccttcag ggaaggtgta ctcggagagg cgagtgtgga cgggcagccc agccaaacca
     3121 ctggctgtgg agctacgaag acaaccactg cccttacatc cagagcctgc taccagccca
     3181 acacccccgt caggaacagg gtcagatcat tctgtctgtt cctcggctgc ccaccctggc
     3241 catggatgaa tacttccact gtgcttttgg gggctacaac agtctagctc aggtggaaga
     3301 accccatgtg gtctgtacca cccctcctca agatcagatg ccacctaatc ctccaggctc
     3361 agatcatgtc accttgcccc tggccctaat gtttgaggat gtggtattga ccgccaccac
     3421 attctccttc tatgactgca gtgctgttca ggccttggaa gtggctgccc cgtgccgcgc
     3481 ttgtgtgagc agcctgtggc ggtgccactg gtgcccccag agtagccact gtatatatgg
     3541 agagcactgc ccagagggtg aaaaggctgt ctacagtgcc caggaggtgg acatcctggt
     3601 tcgtggccca gaggcttgcc cccaggttga gggtctagca agtccccagt tggtgcctgt
     3661 gggttgggag agccatgtga ccctgcacat tcagaacctt cactatttcc aaggtctgcc
     3721 tgccttgtac cactgctggc tagagctgcc tggaaaactg caaaagctac ctgcctcctt
     3781 agaggagaca tccagggact ctggcctcat tcactgccag gcccagcagt tctacccctc
     3841 tatgtctcag tgggagctcc cagtgcccat ctatgtaacc aggggtgaga tccagcggct
     3901 ggacaatgct ggtgatcttc atgtgactct atatgactgt gccatgggtc accctgactg
     3961 cagccactgc caggcggcca atgggagcct gagctgtttg tggtgtggtg atggccagcc
     4021 tgcctgccgc tatgggcctt tgtgcccacc aggtgctgtg gaacagctat gccctatacc
     4081 cagcattgat gtgattgaac ccctgactgg tcccccagaa ggtggcttgg ccatcaccat
     4141 cctgggttcc aacctgggcc aggcctttaa tgatgtgcga aatgctgtaa ctgtggctgg
     4201 ccagccctgc aaccctgacc cttctctcta ccgcatctct gctcggattg tatgtgtgac
     4261 atctcctgct cccaatggca ctgctgggcc agtccaagtg gccattaaga gtcgtcctcc
     4321 tggcatctca acccagaact tcacctacca ggaccctgtc ctgctgagcc tgaatcctca
     4381 gtggggcccc caggcagggg gcacccagct caccatccat gggcagtatc tccagacagg
     4441 aggcaacatc agtgtctttg tgggtgacca accttgccct atccaggagc ctgtgtgtcc
     4501 tgaagccatc atatgccaca ccatgcccca gactgagcca ggagaagcag tggtcctcat
     4561 agtctttggg catgttgaac gcaaactgct caccacccct tttcgataca cagccaatcc
     4621 ccaactggtg gaagcagagc ctagtgtcag cttccgggga ggtggacgag tgattcgagt
     4681 caggggtaca ggcttagacg ttgtgtggca gcccttgctg tctgtgtggc tggaggatga
     4741 gcccaaagta aaggccttgg gggttcaggc ccaggatgcg aacccaagga ggagctgtgg
     4801 tgcccctgct gcagatcctc aggcctgtat ccatcttgag agtggcttac tacagtgttc
     4861 cacgctctgc tctgtcaact catccagcct cctcctgtgc cacagtccag cagtgccaga
     4921 tggggccctc ccaaagcgag tcttctttgc cctagacaac atgcaagtag actttgccag
     4981 tgctagtggg ggccagggat tcctatacca gcctaaccct cgcctggctc cacttagtca
     5041 tgaggggatt actcacccct accacctcaa gccaggccat gtcctagatg tggagggcga
     5101 gggcctcaat ctggggatca gcaaggagga ggtgcaggtg catattggag atggagagtg
     5161 cctggtgaag acactgacgc ttactcactt gtactgtgag ccaccacccc aagcccctca
     5221 acccaccaat ggttcaggca ccctgccgca gtttgtggtg cagatgggca acctgcgcct
     5281 ggctctaggc cctgtccagt acgaagcaga gtccatgatg tccacctttc ctgtgggcgc
     5341 gcctggatct tccggaatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
     5401 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
     5461 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct
     5521 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
     5581 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
     5641 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
     5701 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
     5761 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
     5821 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
     5881 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
     5941 cattgacgtg atcctaggtg ggggccgaaa gtacatgttt cgcatgggaa ccccagaccc
     6001 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
     6061 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
     6121 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
     6181 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
     6241 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
     6301 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga
     6361 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
     6421 tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt
     6481 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
     6541 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
     6601 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
     6661 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
     6721 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
     6781 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggtcatcatc accatcacca
     6841 ttaatctaga gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat
     6901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6961 gtttgtccaa actcatcaat gtatcttatc atgtctggtc gactctagct agactcttcc
     7021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7621 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8941 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9001 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     9061 ctcccggagg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
     9121 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     9361 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     9421 gtatatccat tttcgtcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9481 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     9541 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     9601 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     9661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     9721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     9781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     9841 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     9901 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     9961 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
    10021 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
    10081 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    10141 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    10201 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatt
//
