LOCUS       sequence-135484-        9506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Contactin 1 protein 
            (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to alkaline phosphatase + 
            6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn1-AP-His
FEATURES             Location/Qualifiers
     source          1..9506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6086..6103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6129..6250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6166..6185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6220..6239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6288..6305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6459..7047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6539..6558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7218..8009,8010..8078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7841..7860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8079..8183)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8251..8269
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8307..8329)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8331..8351)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8336..8666
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8517..8652
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8579..8598
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8803..8822
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8889..9344)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9026..9047)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9238..9257
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9470..9492
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9484..9501
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9485..9501
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgaaaatgcc
     1621 attgctggtc agccatctcc tgctaatctc cttaacatct tgtctaggag actttacctg
     1681 gcacagaaga tatggtcatg gagtgtctga ggaggacaaa ggatttgggc ccatttttga
     1741 agagcagcca atcaatacca tttacccaga agagtcactg gaaggaaaag tgtcactcaa
     1801 ctgccgggca cgcgccagcc catttccagt ttacaaatgg agaatgaata acggggacgt
     1861 ggacctgaca aacgatcggt acagtatggt cggaggaaac cttgttatca acaacccgga
     1921 caaacagaaa gacgctgggg tgtactactg cctggcatcc aacaactacg gaatggtcag
     1981 gagcacggag gcaactctga gcttcgggta tctcgacccc tttcctcccg aggagcgtcc
     2041 ggaagtgaag gtgaaggaag ggaagggcat ggtgcttctc tgtgatcctc cctaccactt
     2101 cccagatgat cttagctacc gctggcttct gaatgaattt cctgtattta tcaccatgga
     2161 taagcgaaga tttgtgtctc agaccaacgg caatctctat atcgcaaatg tcgagtcttc
     2221 tgacagaggc aactactcct gctttgtgtc cagtccttct attacaaaga gtgtgttcag
     2281 caagtttatt cccctcattc cgattcctga acgaacaaca aagccatatc ctgctgatat
     2341 tgtggtgcag ttcaaggaca tatacaccat gatgggccaa aatgtgactt tagagtgttt
     2401 cgctctaggg aaccctgttc ctgatatccg gtggcggaag gtgctagaac caatgcccag
     2461 cactgctgag ataagcacgt caggggctgt cctcaagatc ttcaacatcc agctagaaga
     2521 tgaaggcctc tacgagtgtg aggctgagaa catccgggga aaggacaagc accaggcaag
     2581 aatctatgtt caagcgtttc ctgagtgggt agagcatatc aatgacactg aggtggacat
     2641 aggcagtgac ctctactggc cttgcatagc cacagggaag cccattccta ccatcaggtg
     2701 gctgaaaaat ggatactcgt atcacaaagg ggaactgaga ctgtatgatg tgacttttga
     2761 aaatgccggg atgtaccagt gcatagcaga aaacgcgtat gggtccattt atgccaacgc
     2821 tgagctcaag atcctggctt tagctccaac ttttgaaatg aatcctatga agaaaaagat
     2881 cttggctgcg aaaggtggaa gggttatcat tgaatgtaaa cccaaggccg caccaaaacc
     2941 caagttttca tggagcaaag ggacggagtg gctcgttaac agcagcagaa tactcatatg
     3001 ggaagatggt agcttggaaa tcaataatat tacaagaaat gatggtggaa tctatacatg
     3061 ctttgcagaa aataacagag gaaaagctaa tagcactggg actctggtga tcacaaatcc
     3121 cacacgaatt attttggccc caatcaatgc tgacatcact gttggggaaa atgccaccat
     3181 gcagtgtgct gcgtctttcg atcctgcctt ggacctcacg tttgtgtggt ccttcaatgg
     3241 ctacgtgatt gactttaaca aggaaattac gcatatccat taccagagga attttatgct
     3301 cgacgccaat ggggaactgc tcatccgaaa tgcgcagctg aaacacgcag gccggtacac
     3361 gtgcacggcg cagacaatcg tggacaattc ctcggcttcc gctgaccttg tcgttcgagg
     3421 ccccccaggt cccccaggag gcctgagaat agaagacatc agagctacct ctgtggcact
     3481 gacttggagc cgtggttccg acaatcacag ccctatctct aaatacacga tccagaccaa
     3541 gacgattctc tcagatgact ggaaagatgc gaagacagat cccccaatca ttgaaggaaa
     3601 catggagtca gcaaaagcag tggatttgat cccttggatg gagtatgaat ttcgagtggt
     3661 ggcgaccaat actttaggga caggagagcc cagcataccc tcaaacagga tcaaaacaga
     3721 cggtgctgca ccaaatgtgg ctccctcaga tgtaggcggt gggggcggaa ccaacagaga
     3781 gctgacaata acgtgggcgc ctttgtcgag agagtaccat tacggtaaca attttggtta
     3841 catagtggcg tttaagccat ttgatggaga agagtggaag aaagtgactg tgactaaccc
     3901 agacactggc cgctacgtcc acaaagatga gaccatgacc ccctccaccg cgtttcaagt
     3961 caaagtgaaa gccttcaaca ataaaggaga tgggccctac agcctcgtag ctgtcatcaa
     4021 ctctgcacaa gatgctccca gtgaagcccc cacagaggta ggggtcaaag tgttatcatc
     4081 ctctgagata tctgtccatt ggaagcatgt gttagagaaa attgtggaaa gctatcagat
     4141 tcggtactgg gctgggcatg acaaagaagc agccgcacac agagttcagg tcaccagcca
     4201 ggagtactca gccagactgg agaaccttct tcccgacacc cagtacttca ttgaagtggg
     4261 agcctgcaac agtgccggat gcggaccttc cagtgatgtg atcgagacct tcaccagaaa
     4321 agcccctcct agccaaccac caaggatcat cagttcggtg agatctggct cacgatatat
     4381 aatcacatgg gatcacgttg tggcactatc caatgaatct actgtgacag gctacaagat
     4441 actctataga ccggatggcc aacatgacgg caagctgttc tcaacccata aacactccat
     4501 agaagtcccc atccccagag acggagagta tgttgtcgag gttcgagcac acagcgacgg
     4561 gggagacgga gtcgtgtctc aagtcaaaat ttcaggcgtg actagtggat cttccggaat
     4621 catcccagtt gaggaggaga acccggactt ctggaaccgc gaggcagccg aggccctggg
     4681 tgccgccaag aagctgcagc ctgcacagac agccgccaag aacctcatca tcttcctggg
     4741 cgatgggatg ggggtgtcta cggtgacagc tgccaggatc ctaaaagggc agaagaagga
     4801 caaactgggg cctgagatac ccctggccat ggaccgcttc ccatatgtgg ctctgtccaa
     4861 gacatacaat gtagacaaac atgtgccaga cagtggagcc acagccacgg cctacctgtg
     4921 cggggtcaag ggcaacttcc agaccattgg cttgagtgca gccgcccgct ttaaccagtg
     4981 caacacgaca cgcggcaacg aggtcatctc cgtgatgaat cgggccaaga aagcagggaa
     5041 gtcagtggga gtggtaacca ccacacgagt gcagcacgcc tcgccagccg gcacctacgc
     5101 ccacacggtg aaccgcaact ggtactcgga cgccgacgtg cctgcctcgg cccgccagga
     5161 ggggtgccag gacatcgcta cgcagctcat ctccaacatg gacattgacg tgatcctagg
     5221 tgggggccga aagtacatgt ttcgcatggg aaccccagac cctgagtacc cagatgacta
     5281 cagccaaggt gggaccaggc tggacgggaa gaatctggtg caggaatggc tggcgaagcg
     5341 ccagggtgcc cggtatgtgt ggaaccgcac tgagctcatg caggcttccc tggacccgtc
     5401 tgtgacccat ctcatgggtc tctttgagcc tggagacatg aaatacgaga tccaccgaga
     5461 ctccacactg gacccctccc tgatggagat gacagaggct gccctgcgcc tgctgagcag
     5521 gaacccccgc ggcttcttcc tcttcgtgga gggtggtcgc atcgaccatg gtcatcatga
     5581 aagcagggct taccgggcac tgactgagac gatcatgttc gacgacgcca ttgagagggc
     5641 gggccagctc accagcgagg aggacacgct gagcctcgtc actgccgacc actcccacgt
     5701 cttctccttc ggaggctacc ccctgcgagg gagctccatc ttcgggctgg cccctggcaa
     5761 ggcccgggac aggaaggcct acacggtcct cctatacgga aacggtccag gctatgtgct
     5821 caaggacggc gcccggccgg atgttaccga gagcgagagc gggagccccg agtatcggca
     5881 gcagtcagca gtgcccctgg acgaagagac ccacgcaggc gaggacgtgg cggtgttcgc
     5941 gcgcggcccg caggcgcacc tggttcacgg cgtgcaggag cagaccttca tagcgcacgt
     6001 catggccttc gccgcctgcc tggagcccta caccgcctgc gacctggcgc cccccgccgg
     6061 caccaccgac gccgcgcacc cgggtcatca tcaccatcac cattaatcta gagatcttat
     6121 taaagcagaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6241 atgtatctta tcatgtctgg tcgactctag ctagactctt ccgcttcctc gctcactgac
     6301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7801 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8221 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     8281 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga ggaatgtgtg
     8341 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
     8401 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8461 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8521 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8581 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     8641 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcgtcc
     8701 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8761 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     8821 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8881 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     8941 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9001 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9061 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9121 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9181 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9241 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9301 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     9361 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9421 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9481 cgttgtaaaa cgacggccag tgaatt
//
