LOCUS       sequence-135488-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLS-mCherry-NES 17 reporter for light-inducible nuclear export block
            with pDN157 and pDN158.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
  TITLE     Optogenetic control of nuclear protein export.
  JOURNAL   Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
  PUBMED    26853913
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-8"; volume: "7:10624. doi"; pages: " 
            10.1038/ncomms10624"
COMMENT     Sequence Label: NLS-mCherry-NES reporter (pDN160)
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             129..833
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(271..289)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     539..558
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1042..1059)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat ggccgcagca aagcgcgtga
      121 agctggacgt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      181 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      241 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      301 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      361 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      421 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      481 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      541 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      601 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
      661 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      721 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      781 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggtggat
      841 ctggaggttc aggtggaagt gagggagtca tgctgattaa gaaaactgca gaaaatattg
      901 acgagctggc caaggccctg cccgacctga acctggacta gctcgagcgg ccgccactgt
      961 gctggatatc tgcagaattc caccacactg gactagtgga tccgagctcg gtaccaagct
     1021 taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1081 gcccctcccc cgtgccttcc ttgaccctgg aagg
//
