LOCUS       sequence-135491-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  golden gate entry vector for mCherry-LEXY tagging of proteins (no 
            constitutive NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
  TITLE     Optogenetic control of nuclear protein export.
  JOURNAL   Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
  PUBMED    26853913
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-8"; volume: "7:10624. doi"; pages: " 
            10.1038/ncomms10624"
COMMENT     Sequence Label: pLEXY (pDN137)
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             441..746
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     625..644
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(925..943)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tccgccacca tgaagtcttc cgcgtggatc cggcttacta
      121 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
      181 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
      241 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
      301 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
      361 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
      421 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
      481 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
      541 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
      601 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
      661 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
      721 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtgaagac
      781 aagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      841 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      901 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      961 tcgcctggga
//
