LOCUS       sequence-135494-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  golden gate entry vector for mCherry-LEXY tagging of proteins 
            (N-terminal cMyc(P1A) NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
  TITLE     Optogenetic control of nuclear protein export.
  JOURNAL   Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
  PUBMED    26853913
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-8"; volume: "7:10624. doi"; pages: " 
            10.1038/ncomms10624"
COMMENT     Sequence Label: pNLS-LEXY (pDN138)
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             471..776
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     655..674
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(955..973)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tccgccacca tggccgcagc aaagcgcgtg aagctggaca
      121 tgaagtcttc cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
      181 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
      241 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
      301 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
      361 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
      421 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
      481 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
      541 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
      601 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
      661 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
      721 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
      781 caggctccct tatacacagc cagtgaagac aagtgagcaa gggcgaggag gataacatgg
      841 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      901 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      961 tgaaggtgac caagggtggc cccctgccct tcgcctgg
//
