LOCUS       sequence-135499-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively nuclear variant of a very strong, photocaged NES 
            (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear 
            export.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
  TITLE     Optogenetic control of nuclear protein export.
  JOURNAL   Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
  PUBMED    26853913
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-8"; volume: "7:10624. doi"; pages: " 
            10.1038/ncomms10624"
COMMENT     Sequence Label: H2B-GFP-AsLOV2-NES27 (pDN158)
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             105..482
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      495..504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(501..503,504..506,507..1019)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(546..567)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(807..826)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc caccatgcca gagccagcga
      121 agtctgctcc cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag
      181 gcggcaagaa gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc
      241 tgaagcaggt ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt
      301 ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca
      361 agcgctcgac catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg
      421 agttggccaa gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta
      481 aggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      541 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      601 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      661 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      721 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      781 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      841 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      901 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      961 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1021 acggcagcgt gcagctcgcc gaccactacc agcagaacac cccc
//
