LOCUS       sequence-135500-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively nuclear variant of a very strong, photocaged NES 
            (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear 
            export.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
  TITLE     Optogenetic control of nuclear protein export.
  JOURNAL   Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
  PUBMED    26853913
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-8"; volume: "7:10624. doi"; pages: " 
            10.1038/ncomms10624"
COMMENT     Sequence Label: H2B-GFP-AsLOV2-NES27 (pDN158)
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(669..690)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctcgagc tagtccaggt tcaggtcggc cagctccttg
      181 gccagctcct caatattttc tgcagttttc ttaatcagca tgactccctc tctctcggca
      241 gcatctcgga catgctcagt tccatccaac tgaaccccaa taaagtactg gacatctccc
      301 ttctgatctc gcataggctg caagtgaaag aggttccaga actttttacc actctttgta
      361 taattaatca gctgaacagt gacctctgtt tggttatcta tggcatctct aatttttctc
      421 actgtcgcgc gatcagtttc aggaccttgt agaaacctgc agtttcttcc caaaatttct
      481 tcacggctat attctgtcaa ctgcaagaaa ctatcggacg cgaatataat gggattatct
      541 ggcaatcttg ggtcagtaat gacaaagttc ttctcaatac gttcaagtgt agtagccaaa
      601 cttccacctg aacctccaga tccacccttg tacagctcgt ccatgccgag agtgatcccg
      661 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      721 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      781 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      841 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      901 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      961 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     1021 ttgtagttgc c
//
