LOCUS       sequence-135506-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reb_1 with RebA(Q91R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Polka JK, Silver PA
  TITLE     A Tunable Protein Piston That Breaks Membranes to Release 
            Encapsulated Cargo.
  JOURNAL   ACS Synth Biol. 2016 Feb 3.
  PUBMED    26814170
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 3."
COMMENT     Sequence Label: Reb_79 aka PAS620
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagttag
       61 cctcgtggaa agtttttcac taagttttgt gtcatctcat taaatttact gagattagcc
      121 acttcagcat ctcgattact gctaatggat ttcatgattc cttcccatat tttaatttga
      181 ggttcgttgt cgattgcggc agcttcagcc attttttgca tagcaagtgc tagcaccgta
      241 tttgatgttg tttcaaagcc agaatgcata gccagttcat tttgcacagc gctagcggca
      301 gccattgaaa caatatcata agcaccttga gaggcgacat tatttttagg atccatcatc
      361 cgttgattga tatcttctga gaaatgttct atatccgcca tttacaagat tatcccatca
      421 ttttcataat acgttcacag gcaacagaag tgacggcttg accgatttga tttaactgtt
      481 gagagcttga ttgagcattt tgaatagcta aattagcggt tgaagcaacg ccttgataca
      541 gcacacccat tgcttgagct ggtgccatac ctataacatt catcatattt gaagtgacag
      601 aatcggtcac ttgtggattc acataaagat tgggtgttat tgcattatct gccatagttt
      661 gaagctctag ttaaaatgga ttgatatcaa aaagcaaaat aaaaaaataa aataaggaaa
      721 acgatagcgt tgggtcagga agtaatacgt tatgcgttga ggagaaactc aataatacaa
      781 atcaacgtat acttcctaac cccagctttc ttcccccacg agaacaatta accgtctctg
      841 atgattttta tctactttaa atgattttat ttcgcttatc atactttaat ttagccaatt
      901 tatctgattg gcattatttt agcatatcga aataagcatt aaccattttt agcggcttta
      961 ataacagcaa gggcatcagt taaacggctt gtgtcattag aacggttcac tgaaccaatg
     1021 gcttgagcat tagctgccgt gtcaacagaa tatagaatac tcataccttg agtggttgat
     1081 gcc
//
