LOCUS       sequence-135508-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type R bodies from C. taeniospiralis in pETM11 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Polka JK, Silver PA
  TITLE     A Tunable Protein Piston That Breaks Membranes to Release 
            Encapsulated Cargo.
  JOURNAL   ACS Synth Biol. 2016 Feb 3.
  PUBMED    26814170
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 3."
COMMENT     Sequence Label: Reb_1 aka PAS619
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggctttggt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag ttagcctcgt
       61 ggaaagtttt tcactaagtt ttgtgtcatc tcattaaatt tactgagatt agccacttca
      121 gcatctcgat tactgctaat ggatttcatg attccttccc atattttaat ttgaggttcg
      181 ttgtcgattg cggcagcttc agccattttt tgcatagcaa gtgctagcac cgtatttgat
      241 gttgtttcaa agccagaatg catagccagt tcattttgca cagcgctagc ggcagccatt
      301 gaaacaatat cataagcacc ttgagaggcg acattatttt taggatccat catccgttga
      361 ttgatatctt ctgagaaatg ttctatatcc gccatttaca agattatccc atcattttca
      421 taatacgttc acaggcaaca gaagtgacgg cttgaccgat ttgatttaac tgttgagagc
      481 ttgattgagc attttgaata gctaaattag cggttgaagc aacgccttga tacagcacac
      541 ccattgcttg agctggtgcc atacctataa cattcatcat atttgaagtg acagaatcgg
      601 tcacttgtgg attcacataa agattgggtg ttattgcatt atctgccata gtttgaagct
      661 ctagttaaaa tggattgata tcaaaaagca aaataaaaaa ataaaataag gaaaacgata
      721 gcgttgggtc aggaagtaat acgttatgcg ttgaggagaa actcaataat acaaatcaac
      781 gtatacttcc taaccccagc tttcttcccc cacgagaaca attaaccgtc tctgatgatt
      841 tttatctact ttaaatgatt ttatttcgct tatcatactt taatttagcc aatttatctg
      901 attggcatta ttttagcata tcgaaataag cattaaccat ttttagcggc tttaataaca
      961 gcaagggcat cagttaaacg gcttgtgtca ttagaacggt tcactgaacc aatggcttga
     1021 gcattagctg ccgtgtcaac agaa
//
