LOCUS       sequence-135515-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reb_1 with RebB(I67T, N84I).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Polka JK, Silver PA
  TITLE     A Tunable Protein Piston That Breaks Membranes to Release 
            Encapsulated Cargo.
  JOURNAL   ACS Synth Biol. 2016 Feb 3.
  PUBMED    26814170
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 3."
COMMENT     Sequence Label: Reb_80 aka PAS621
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt agcctcgtgg aaagtttttc
       61 actaagtttt gtgtcatctc attaaattta ctgagattag ccacttcagc atctcgatta
      121 ctgctaatgg atttcatgat tccttcccat attttaattt gaggttcgtt gtcgattgcg
      181 gcagcttcag ccattttttg catagcaagt gctagcaccg tatttgatgt tgtttcaaag
      241 ccagaatgca tagccagttc attttgcaca gcgctagcgg cagccattga aacaatatca
      301 taagcacctt gagaggcgac attattttta ggatccatca tccgttgatt gatatcttct
      361 gagaaatgtt ctatatccgc catttacaag attatcccat cattttcata atacgttcac
      421 aggcaacaga agtgacggct tgaccgattt gatttaactg ttgagagctt gattgagcat
      481 tttgaatagc taaattagcg gttgaagcaa cgccttgata cagcacaccc attgcttgag
      541 ctggtgccat acctataaca ttcatcatat ttgaagtgac agaatcggtc acttgtggat
      601 tcacataaag attgggtgtt attgcattat ctgccatagt ttgaagctct agttaaaatg
      661 gattgatatc aaaaagcaaa ataaaaaaat aaaataagga aaacgatagc gttgggtcag
      721 gaagtaatac gttatgcgtt gaggagaaac tcaataatac aaatcaacgt atacttccta
      781 accccagctt tcttccccca cgagaacaat taaccgtctc tgatgatttt tatctacttt
      841 aaatgatttt atttcgctta tcatacttta atttagccaa tttatctgat tggcattatt
      901 ttagcatatc gaaataagca ttaaccattt ttagcggctt taataacagc aagggcatca
      961 gttaaacggc ttgtgtcatt agaacggatc actgaaccaa tggcttgagc attagctgcc
//
