LOCUS       sequence-135517-        9878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the L1CAM protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: L1cam-AP-His
FEATURES             Location/Qualifiers
     source          1..9878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6458..6475
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6501..6622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6538..6557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6592..6611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6660..6677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6831..7419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6911..6930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7590..8381,8382..8450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8213..8232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8451..8555)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8623..8641
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8679..8701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8703..8723)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8708..9038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8889..9024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8951..8970
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9175..9194
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9261..9716)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9398..9419)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9610..9629
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9842..9864
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9856..9873
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9857..9873
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggtcgtgat
     1621 gctgcggtac gtgtggcctc tcctcctctg cagcccctgc ctgctcatac agattccaga
     1681 cgaatataaa ggacaccatg tgctagagcc acctgtcatc acggaacagt ctccacggcg
     1741 cctggttgtc ttcccaacag atgacataag cctgaaatgt gaagccagag gcagacccca
     1801 agtggagttc cgctggacga aagatggcat ccacttcaaa cccaaggaag aattgggtgt
     1861 agtggtgcat gaggcaccct attctggctc cttcaccatc gaaggcaaca acagctttgc
     1921 ccagaggttt cagggcatct atcgctgcta tgccagcaat aagctaggaa ctgccatgtc
     1981 gcatgagatc cagctcgtgg ccgagggtgc ccccaagtgg ccgaaggaga ctgtaaaacc
     2041 tgtggaagtg gaggaaggag aatcagtagt tctgccttgc aaccctccac ccagtgcagc
     2101 cccacttagg atctactgga tgaacagcaa gattttgcac atcaaacaag atgagcgggt
     2161 gtccatgggc cagaatggag acctatattt tgccaatgtg cttacctcag acaatcattc
     2221 agactacatc tgcaatgccc acttccctgg tacccggacc atcattcaaa aggaacctat
     2281 tgacctccgg gtcaagccca ccaacagcat gattgaccgg aagccacgtc tgctctttcc
     2341 cacaaactcc agcagccgcc tggtagcctt gcagggccag tcattgatcc tggagtgcat
     2401 tgctgaggga ttccctacac ccaccatcaa gtggctgcac cccagtgacc caatgccaac
     2461 agaccgtgtt atctaccaaa accacaacaa gaccctgcaa ctactcaatg tgggcgaaga
     2521 ggacgatggc gagtatacct gccttgctga gaactcgctg ggcagtgccc ggcatgccta
     2581 ctatgttact gtggaagctg ccccatattg gctgcagaag ccccagagcc atttgtatgg
     2641 tccaggagag actgcccgcc tagactgcca agtccagggc aggccccaac cagagatcac
     2701 ttggagaatc aacggaatgt ctatggagac ggtgaacaag gaccagaagt accggattga
     2761 gcaggggtct ctgatcttga gtaacgtgca gccaagtgac acaatggtga cccagtgtga
     2821 agcccgcaac cagcatgggc tcctgctagc caatgcctac atttatgttg tccagctgcc
     2881 agccaggatc ctaacaaaag acaatcagac atacatggca gttgagggca gtactgctta
     2941 cttgctgtgc aaagcctttg gagctcctgt tcccagtgtc cagtggctgg atgaagaagg
     3001 aaccacagtg cttcaggatg aacgattttt cccctatgcc aatggaacgc tgagcatcag
     3061 agacctccag gccaatgaca ctggacgcta tttctgccag gctgccaatg accagaacaa
     3121 tgtgaccatt ttggctaacc tacaggttaa agaagcaacc cagatcacac aggggccccg
     3181 gagcgcaatt gagaagaaag gtgcaagggt gacattcacg tgccaggcct cctttgaccc
     3241 ctctttgcag gccagcatca cttggcgtgg agatgggaga gacctacagg aacgtgggga
     3301 cagtgacaag tatttcatag aagatgggaa actagtcatc cagagcctgg actacagtga
     3361 ccagggcaac tacagttgtg tggccagcac tgaactggat gaggtggaga gcagggcaca
     3421 gctcttagtg gtggggagcc ctgggccagt gcctcacctg gagctgtccg accgccacct
     3481 gctgaagcag agccaggtgc acttgtcttg gagccctgct gaagaccaca actctcccat
     3541 tgagaagtat gacattgaat ttgaggacaa ggaaatggct cctgagaaat ggttcagtct
     3601 gggcaaggtg ccaggaaatc agacctctac taccctcaag ctgtccccct atgtccacta
     3661 cacctttcgg gtcactgcca ttaacaaata tggtcctgga gaacccagcc ctgtctctga
     3721 gactgtggtc acacctgagg cagccccaga gaagaaccct gtggatgtga gaggggaagg
     3781 gaatgagacc aacaatatgg tcatcacatg gaagcccctt cggtggatgg attggaatgc
     3841 cccccagatt cagtaccgtg tacagtggcg tccacagggc aagcaggaga cctggaggga
     3901 acagaccgtg agcgaccctt tcctggtggt gtctaacact tccacatttg tgccttatga
     3961 gatcaaagtc caggcagtga acaaccaggg caagggccct gagccccagg tcaccattgg
     4021 ctattcaggg gaagactacc cccaggtgag ccctgaactt gaagacatca caatcttcaa
     4081 ctcaagtact gtgcttgtca ggtggaggcc tgtggacttg gcccaggtta agggccacct
     4141 caagggatac aatgtaacat actggtggaa gggcagccag agaaagcaca gcaagaggca
     4201 tatccacaaa agccacatag tggtacctgc aaataccacc agtgccatcc tcagtggttt
     4261 gcgcccttac agctcttacc atgtggaggt acaggccttt aatgggcggg gcttggggcc
     4321 tgcgagtgaa tggaccttca gcaccccaga gggagtgcct ggccaccctg aggcattaca
     4381 cctggagtgt cagtcggaca ctagtctgct actgcactgg cagccaccac tcagccacaa
     4441 tggagtgctc actggctacc tgctctctta ccatcccgtg gaaggggaaa gcaaagagca
     4501 gttgttcttc aacctttcgg acccagaact ccggactcat aatctgacca acctcaaccc
     4561 tgatctacag taccgcttcc agcttcaggc caccacccaa cagggtcctg gtgaggccat
     4621 cgtgcgtgaa ggaggcacca tggccctgtt tggcaagcca gattttggca acatctcagc
     4681 cacagcaggt gaaaactaca gcgtggtctc ctgggtccct cggaagggcc agtgcaattt
     4741 caggttccat atcttgttca aagccttacc agaagggaaa gtgagccctg atcaccagcc
     4801 tcagcctcag tatgtcagct acaatcagag ctcctacaca caatggaacc tacagcctga
     4861 caccaaatat gagatccacc tgataaagga gaaggtcctc ctgcaccatc tggatgtgaa
     4921 gactaatgga actggccctg tgcgagtttc tactacaggt agctttgcct cctctagtgg
     4981 atcttccgga atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc
     5041 cgaggccctg ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat
     5101 catcttcctg ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg
     5161 gcagaagaag gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt
     5221 ggctctgtcc aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac
     5281 ggcctacctg tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg
     5341 ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa
     5401 gaaagcaggg aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc
     5461 cggcacctac gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc
     5521 ggcccgccag gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga
     5581 cgtgatccta ggtgggggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta
     5641 cccagatgac tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg
     5701 gctggcgaag cgccagggtg cccggtacgt gtggaaccgc actgagctca tgcaggcttc
     5761 cctggacccg tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga
     5821 gatccaccga gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg
     5881 cctgctgagc aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca
     5941 tggtcatcat gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc
     6001 cattgagagg gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga
     6061 ccactcccac gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct
     6121 ggcccctggc aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc
     6181 aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc
     6241 cgagtatcgg cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt
     6301 ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt
     6361 catagcgcac gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc
     6421 gccccccgcc ggcaccaccg acgccgcgca cccgggtcat catcaccatc accattaatc
     6481 tagagatctt attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca
     6541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6601 ccaaactcat caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc
     6661 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6721 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6961 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7021 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7081 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7141 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7201 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7261 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7321 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7381 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7441 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7501 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7561 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7621 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7681 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7741 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7801 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7861 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7921 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7981 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8041 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8101 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8161 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8221 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8281 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8341 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8401 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8461 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8521 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8581 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8641 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8701 gaggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     8761 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     8821 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     8881 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8941 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     9001 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     9061 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     9181 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     9241 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     9301 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     9361 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     9421 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     9481 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     9541 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     9601 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     9661 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     9721 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9781 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9841 tcccagtcac gacgttgtaa aacgacggcc agtgaatt
//
