LOCUS       sequence-135529-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reb_1 with RebB(T28A, D72G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Polka JK, Silver PA
  TITLE     A Tunable Protein Piston That Breaks Membranes to Release 
            Encapsulated Cargo.
  JOURNAL   ACS Synth Biol. 2016 Feb 3.
  PUBMED    26814170
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 3."
COMMENT     Sequence Label: Reb_81 aka PAS622
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt tagcctcgtg gaaagttttt
       61 cactaagttt tgtgtcatct cattaaattt actgagatta gccacttcag catctcgatt
      121 actgctaatg gatttcatga ttccttccca tattttaatt tgaggttcgt tgtcgattgc
      181 ggcagcttca gccatttttt gcatagcaag tgctagcacc gtatttgatg ttgtttcaaa
      241 gccagaatgc atagccagtt cattttgcac agcgctagcg gcagccattg aaacaatatc
      301 ataagcacct tgagaggcga cattattttt aggatccatc atccgttgat tgatatcttc
      361 tgagaaatgt tctatatccg ccatttacaa gattatccca tcattttcat aatacgttca
      421 caggcaacag aagtgacggc ttgaccgatt tgatttaact gttgagagct tgattgagca
      481 ttttgaatag ctaaattagc ggttgaagca acgccttgat acagcacacc cattgcttga
      541 gctggtgcca tacctataac attcatcata tttgaagtga cagaatcggt cacttgtgga
      601 ttcacataaa gattgggtgt tattgcatta tctgccatag tttgaagctc tagttaaaat
      661 ggattgatat caaaaagcaa aataaaaaaa taaaataagg aaaacgatag cgttgggtca
      721 ggaagtaata cgttatgcgt tgaggagaaa ctcaataata caaatcaacg tatacttcct
      781 aaccccagct ttcttccccc acgagaacaa ttaaccgtct ctgatgattt ttatctactt
      841 taaatgattt tatttcgctt atcatacttt aatttagcca atttatctga ttggcattat
      901 tttagcatat cgaaataagc attaaccatt tttagcggct ttaataacag caagggcatc
      961 agttaaacgg cttgtgtcat tagaacggtt cactgaacca atggcttgag ca
//
