LOCUS       sequence-13553-e       20411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 20411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1266
FEATURES             Location/Qualifiers
     source          1..20411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..67
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     regulatory      4131..4140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4137..4139,4140..4142,4143..4853)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4137..4139,4140..4142,4143..4853)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4182..4203)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4443..4462)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4790..4811
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4866..4883
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             4896..4916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4920..4940
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4944..4964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4971..5018
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    5141..5222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5178..5197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12867..12888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13051..13155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13156..13224,13225..14016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(18223..18245)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18331..18471
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18386..18405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18486..18503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18657..19245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18737..18756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19416..20207,20208..20276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(20277..20381)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt
      121 cgagctccta ggccggcctt tgcaaataaa taaactggac ctttttgttt aattatagtt
      181 caaaacagca aaaaaagcaa gaataaataa aatgtattag tccaaaaatt tcattttaga
      241 aatatttgta tcctccttct tgccaaattg catttactaa tacacagatt tcatatttac
      301 cacacactta actttttgtt gtctagtaaa aataagaagt aaaagtaatc aaaagcttgg
      361 cagatggggg caagtgagaa gtaatgtaca cagataattc agaaacagga aatgcctaaa
      421 taataaaact ttgaccacat atcatttata gaaagtatgt caattgaaca aacaaaagaa
      481 gcataattat acaaataaaa cttggggatc tcaaaaaatt ctaacacaat cttattttat
      541 tctcttaaaa ggttcaatac agtatgatta ggataataaa atctagtttc aaaaaatata
      601 ataaatctaa aacaaggaaa aaactaaaaa ctgtgcattt gttctctaat ctttttacca
      661 tttttcaatc tgggaacact tctaaattta ggagtaagct ataataagcc acgatcatat
      721 gactgcctag tttcttgaac attaataaat acttaattcc agcttacatg aaatattgca
      781 tttggagagg tgggtactat gtagtataaa aactaagtcg aatcagtaac tttaactttt
      841 cttcttgttg gtttcctttt atttttaaat cccagaaccc aaagatttct aaagattgtg
      901 taagctaaat tatttgtaca taacaaaaac tatatggctt ttctaatact gtaagcttag
      961 aaaatcccag tatatttaac caagcatttt ccttttatct ttttgaattt taaccatttt
     1021 gaatcctaca gctggtgaat tttcatattc tacaattatg tttctcattg cattctatct
     1081 tctagttatg gtataatgtt cccacatata aatattatgt aaaacacaaa tgcaatcaat
     1141 taatattaac ttattcctag ggataataat agcaataata actcgtcata ctttagtgaa
     1201 ttctgaatct gagtgaaaca agaataccaa gtttctatga tatttctatt gcaatatacc
     1261 gaatacaaca acttctcaaa tatgtaaatt aatttgtgac tcagtttgct gggtaatgtt
     1321 cacatcatga ctcaaataga tgcaactact taaaaatgaa tgacttggga ggctgccatg
     1381 gactatgaag ctgtgtgagt aagtaagctg cttgtatctg gcctatattg agaggaagaa
     1441 atttttccat ctgacatatt tagaggtaac acaattgagt aaagtggctt agatatttga
     1501 tctttgaaat gtcattgcag aagataaaaa tgttgtggat ataatgtctg tgaacatgag
     1561 gatattagaa tgttataaat gtgttccata tgtttaagag tcaaagcaac acatagaaat
     1621 cattttttgg aagagtagag atacagagtt tatcaatgtc atcccactga tggcaggaga
     1681 agaaaggtcg tatatacagt ctgctggtcg aaattaaagg catcaccagg atgagaaaat
     1741 tcgttgaaaa tgatggtgtg tgagatgaaa taatcattcc ttttgacaat tatgaagtta
     1801 ggatagagag tgtgggaggt gtaagggtta caaagaggat taggtgtaag aataacaagg
     1861 cagagtggga ggacagcatt tagtgcagat ggtaattaat gtatcgtggc tatattgttg
     1921 ggcttattaa aactggttaa agagatgatt ttaaagctgg tgaagatgaa catttttcaa
     1981 ataactttta caaagaacag gtgttacctt tttatcaaaa ttaggaatgg ttgatcaatt
     2041 tatgaaatga aacatgaatt aaaggagata taaccgcaga gggtagattt caacttgtag
     2101 gagttaagcc agaaatttat ttgggtttaa aatgtagatg tcatttgttt acttgaaaaa
     2161 gccccaattt tgtgtgttac gtaaaaataa gtacaactag aataacaaga ggttaggttt
     2221 tgtaacatct gtgtacaaat tattattagc attgctaatc caagatctta atcactggca
     2281 tgatacctat atcaccacca gatgtgagct ttatgaacct aacagtaaat aaatgattta
     2341 gaataacttt agtataattt ttaaaggaaa caaaaatgta aacatttttt ccccaaagct
     2401 agctcattgg tccaaagcta gcccatttat aaatgaatat tttaaaaatg tgccattgtg
     2461 tttctaaatt tcagttttcc tttcatataa aacaaaaata gacatccatg aatagtagtt
     2521 gttatgaaaa cagaatttta gtggatgtac caacataatt agaaaaaaat acagaaagat
     2581 tttcaaacaa actcattcct caagacaaga aactaacttt tgttaacaat ggaaagctac
     2641 caccagccac atctgtcaat gaaatgtttt gtagacaggc ttggaataag atattttagt
     2701 ggcccgtgct atttatttgt gccaacatct ttttaaattg gtttttttct attcctcttt
     2761 agattctttt tccttttaaa aattattggc atggatgctt tttctcccct ccagattata
     2821 taaacattaa acacacacac gtacacacac acacacgttt tcagtagcga ttactgtgtt
     2881 gacaccactt ccactctgtc tccccagatc agcgcttctg acgagcccat ttgcacgggg
     2941 actcggccct ctcttttcct ggcgaggctt ttgaacctgt cgccaagcca gtacctcaga
     3001 gagaaggaaa tgcagaggga cgagaaggat gcatggcgcc tgcccccagt tagatgttct
     3061 tcagataatc tgcaaccaag tgcgctgata aaatatttaa taaagagccg gtaaacatca
     3121 ctgcaaatac attaaggcat ttcagcccga cagcttatga ttatttcaaa acttaaaaag
     3181 gagatcatag attgtcactg tttctgtact caaccaagaa accgaacctt cctgaggtgg
     3241 gtggattggt gggtgagttg ctgcttttcg ccccctctcc tccccccttc tgcttgtcgc
     3301 tgtggctctc agcagcaggg atttatttat ttatgtttgc agtttaaaag gtgggggtgg
     3361 gggcgccgag gacatgtagg atctgctcac aaagcaccag ctcaggtgtt gtgtgctttt
     3421 gtattagctg gaaaagacag actctggaac ttttacaggg cgagaagagg ccaccggctc
     3481 gcgctttgta tactttgcac ttgaaatcgt tacattcaac tgaatatttg gctcattcag
     3541 atccgaaaag tctcctagca tcgtcgaatt ccctcctccg cccctccgct ctaaaggggg
     3601 gaaggggggg aaggtaggaa atgtttagta aattccacat ctttgaatct tcctaaagca
     3661 gtggtcacag agcttaaagg tgacacaaca gtgctcacgt aaaccaacac acattcccca
     3721 ccccccaagc caacaataaa atcatcccct tcaatttgcc atgatcgtcg ctaccaggag
     3781 ccaggtgatt atcctaatta atgtctatct aattaaatta ctgtcagcag ctaaccaatg
     3841 gcaggagccg tttcatcggc tgcacaagca gcaagatcaa aagtgagcct tttctgattg
     3901 ctgcatagtg tcaattggcc aatctcttct cccagggaaa aaaaaagtaa atcaaacctt
     3961 tgagaagcat ttgctggttg aagtgctttc tgtctagtga gggggtctgt ggatttctag
     4021 tttatgataa ataggacttt aaaaaccagg gacgggaggg cgagtgttca ggtggcgcgc
     4081 cgaagttcct attccgaagt tcctattctt caaaaggtat aggaacttcc gccaccatgg
     4141 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4201 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4261 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4321 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4381 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4441 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4501 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4561 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4621 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4681 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4741 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4801 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagttatttc
     4861 tgaactgctg cccgggctgc tgcatggaac cggatccaaa aaagaagaga aaggtagatc
     4921 caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtaatataa gaagttccta
     4981 ttccgaagtt cctattctct agaaagtata ggaacttcag cggccgcgac tctagatcat
     5041 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5101 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5161 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5221 gcgaattctc gtaaaactct tcatggcagt agttattatt ctctctctct ctctttttct
     5281 tttttcttga gacaggatat ttctctgttg cccaggctgg agtgcagtgg cacagtcttg
     5341 gctcactgca gcctggacct cctgggctca agccatcctc ccacctcagc ctcccaagta
     5401 gctggggcta caggcacatg gccaccaggc ccagataatt tttcattttt gtagagactg
     5461 agtctcacca tgttacccag gtttattatt ctcatttttt agatgaagag actgaggtcc
     5521 agagaagctc aatgacttgc ctagttttac aaatctcctg ccatcacata cccctcagcg
     5581 tccttaataa gagggaggcc accaactatg tgctgggcac tgtggtggat gctggagcta
     5641 tagggttgag tatataagaa atggtgttgc tggagcaact gttgcttgct tacctgacct
     5701 atctgagaat taattagcag gggaacatat ttttgttttc agattcaata taagaacttg
     5761 tgtgggcaaa aataaagatc agtagtaata acagtagttc ccatttgctg actgtactgt
     5821 cctaagtgca tatatatata catacacaca cgcataccta tactcctcta atactcaaaa
     5881 tgatcctgtt tatgtattgt taatatgctc attttatttt taaattttta tttattttta
     5941 tttttattta tttttgagac ggagtctcat tctgtcgcgg aggctgaagt gcagtggtgc
     6001 gatctcagct cagtgcgacc tccgcctccc gggttcaagt gattctcctg cctcagcctc
     6061 cggactagct gggattacag gcgcccgcct ccacgcccag ctaatttttg tatttttagt
     6121 agagatgggg tttcgccatg ttggccaggc tggtctcgta ctcctgacct tgagtgatcc
     6181 acctgcctcg gcctcccaaa gtgctgggat tacaggcatg agccaccgcg ccgggctaat
     6241 atgctcattt tagtgaggca aaaatagagg ctcagagtct gatttgtaca aaactacaga
     6301 gcagttaagt gtcctctcag atgtgtaccc tgatctgggt gactctagga ctctaggtct
     6361 caactgttac aaccagttaa gggtttgggg aagcactggg ccaagagtca ggaaaatgga
     6421 agccacaggt agtgcaaggt cttgggaatg ggacgtctgg tccaaggatt cacgcgatga
     6481 ctggaacccg aagagccggg gcccggttta cggccgccat gaagcaacgc gcgccggtag
     6541 gtttgggaat cagggagccc tctgaatagg agactgagtt gggagggaaa ggggcttcgc
     6601 tgggggagcc tcggcttctt ctgggagaaa attcccacgg ctacctagtg agcctgcaaa
     6661 ctggtaggcg ccggcgtagg cgcgcgggcg gggccggggg cggggcctgc ggggcgtggc
     6721 ggggcgggca gagggcgggg cctgcttctc ctcagcttca ggcggctgcg acgagccctc
     6781 aggcgaacct ctcggctttc ccgcgcggcg ccgcctcttg ctgcgcctcc gcctcctcct
     6841 ctgctccgcc accggcttcc tcctcctgag cagtcagccc gcgcgccggc cggctccgtt
     6901 atggcgaccc gcagccctgg cgtcgtggtg agcagctcgg cctgccggcc ctggccggtt
     6961 caggcccacg cggcaggtgg cggccgggcc ctgaggcgcg ggatcgatcc atgacacccc
     7021 cccgcccccc atatctcact ataattcact gtcaaatggc ttttatgatt tggagtttgg
     7081 ggatactttt aaagacaggg tcttggtagc tcctagatgg cttgggattc acttagtaac
     7141 ccttgctggc ctaaaattca ggaacttcta cctcatcttc ccaagtactg ggttagtagg
     7201 gatgtccagc atatttagct tgctttaatg attttgctgt acttttttct gtgtgtacag
     7261 gtgcatgtat atttataaaa ctaacataat atattttacc cccaaagtac cataacccta
     7321 attaatgcta ttaaatgttt tgtatgctgg gctctgggca tactaatata accaatatta
     7381 tacaatgcaa aacaattttg gaatatcttc tgtaaaatta tcttattagt aagttgtaac
     7441 catctcagac ttctaagttt gagtttgtaa aggatcagtt ttttttggtg acctacacac
     7501 acacacacac acacacacac acacacacac acaggggggt ggattctgag aatttagaga
     7561 atttagatgg ttaataccag tttttatttt tattctgtgt gatatttatt tattaagatt
     7621 tatttattta ttacatgtag gtacactgta tctgtcttca gacacaccag aagagggcat
     7681 cagatctcgt tacagatggt tgtgagtcac catgtggttg ctgggatttg aactcaggac
     7741 ctctggaaga acagtcagtg ctcttaaccg ttgagccatc tcaccagccc cgtgatattt
     7801 tatttaactt actagaatat actgtgttaa aacatatttt aggggattgg ggagatggcc
     7861 cattctgtaa agtgtctgcc atgtcatctt caggacctgc gtttgtaacc ccagcatgga
     7921 gaagacagag acagggagac ccctgggctt cctggcaagc tagactagca aatttggtta
     7981 actaggttca atgagagact cagtctcaaa acataagaag tgaatttttt agacccaatt
     8041 tcaatctctg aactccatac gtatgcacat gtacctgcat ataaatatat atacacaccc
     8101 ataaacacat gcacacacaa aaaatggttc aaaagttctt aaaatgtata agagtagaaa
     8161 gtgtagagcc gggagtggtg gcgcacgcct ttaatcccag cacttgggag cagaggcagg
     8221 tggatttctg agttcgaggc cagcctggtc tacagagtga gttccaggac agccagagct
     8281 atacagagaa atcctgcctc aaaaaaccaa aaataaagtg tatatactaa tctttcatgc
     8341 ctaaaatatt tatttttgcc acatttttca tacctcttta atatttttct ttttctcttt
     8401 gtgcttcaaa ggaaattttg tgtcatgttt tacttttcat tcttgactct attcagcagt
     8461 aagacgcagc atggctttct tagagaagta taatctatta caaaacggta acatttaact
     8521 gctctacata ccctcttgta gctaaattcc tctgttaaac taagacttat tatctaaacg
     8581 taattaaatg ttgatatctt ataatgctcc actttgaaac agctgtcttt cttttgcaga
     8641 ttagcgatga tgaaccaggt tatgacctag atttgttttg tatacctaat cattatgccg
     8701 aggatttgga aaaagtgttt attcctcatg gactgattat ggacaggtta gtaagacctc
     8761 gattgaagtt atttttagag ggtgtgtgtg tggtgtgtgt gtgtgtgtgt gtgtgtgtgt
     8821 gtgtgtgtgt gtaagagaga gaggagaggc atgtgtgata tatatgtgaa gtgtattgca
     8881 tgtacatgga atccagagaa ggacattagg tgtcctactc tgttgctctc ttattccctt
     8941 gaatcagtgt ctcttgctga acctggggca tacatactga tttggctagg ctgcctggct
     9001 agcaagaccc agggatctgc ttgtctctgc cccccagtgc tgacgttaca agcaggtgca
     9061 accacatctg gtttttatat gggtactggg ggatctgaac ttagggcctg atgcgtgccc
     9121 aagtgctctc acccgccaag ccacctctcc agccaaaggg ttttttttct tgtattaatt
     9181 ctaacttagc aactgttgag tagttgatgt ttgaatataa gctaagccaa cttattagaa
     9241 taaatgtttt ctatttttta cttgtatgtg tgagtgcctg catgtatgta tgtgaagcat
     9301 atgtattcca ggatgtcaga attgtgatgg attccctgga actggagtta cagaccattg
     9361 tgaaccactg tgtatgggtg ctgggaactg aactttaggt cctctgcaaa aacagtaagg
     9421 atcttaactg ataaactatc tttctagccc taaggctttc tacttttaaa gttcgtttct
     9481 ctttctctct ctctctctct ctctctctct ctctctccct ctccctccct cctcctcctc
     9541 ctcctctttc ccccctctcg cctcctcttt ctctctcccc tccctctctc tctctcacac
     9601 acacacacag acagagagag gagagagaaa ggaagcctta ctaattaatt acctagtttt
     9661 agacataact agtgggaatc catagtaagt aaatcaacat tcttgaagag aggttagaag
     9721 aattggctct cgttgtccat cgaggctgta taagattatt cttctgtttt ctcagcttac
     9781 catacatatt ttctacgtag acaataccaa ttcaaataca ctgtacgtaa agtatatgcg
     9841 cacacacaca cacacacaca cacacacaca cacacaagtt acatattgtc atatagtact
     9901 aaaatttatg gactttttca tattaataca tctagagcta tcatgttttt tatcttttaa
     9961 aaattacttt acggggctac agagatgtct cagcagttag gagcactgac tgctcttcca
    10021 gaggtcctga gttcaattcc cagcaaccac atggtggctc acaaccatct gtaatgggat
    10081 ctgatccact cttatggtgt gtctgaagac agtgacagtg tactcatata catgaaataa
    10141 atctttaaaa aattacttta ccatcagggt atatatatat atatatatgt gtgtgtgtgt
    10201 gtgtgtgtgt atgaagtatt tgctatttga gagggtctca ctgtttagct cagtctggct
    10261 tggaacgttc tgtatagccc agactggcct caactcagca gtccccctga aaggctctca
    10321 tatgtggaga ttacaggtat gtgctactgt gcccagccct acatgttact ttgtttttga
    10381 aatagggtat caaatagctt agactggctt ctaagtcagt gtgtagctaa ggatggtctg
    10441 agcttctgaa cctgctactt caactcctgg tgtgccatca tggccagttt gcacagttgt
    10501 gagagtagaa ctcaggacct cctatgtgtt actagacatg ttctctacca gctgagctac
    10561 atcccaattc gcctacattt acttttattt tttaaaagat ttatttatgt atacagcact
    10621 ctgcctgcat gtacatctgc atgccagaag agggcaccag atctcatcat acatagttgt
    10681 gagccaccat gtggttgctg gggattgaac tcagggcctc tggaagagca gtcagtgctc
    10741 ttaacctctg agccatctct gtagccccct acatttattt taaagattat aggctattct
    10801 attcatagat gtgccataac tgatttaacc agatcttcat tattttgtac ttagtagagt
    10861 atattagagg gaatgaatct taaatacaca ttttgacact tgctcagctt tagacaagta
    10921 aaaatcactg gtcaaggaaa tggtgcttag tggatgcttc tgaactgtca aattgtcctc
    10981 caacaagtta catcatttta tacttccaac gttatgtgag agttcatttt tctttaggct
    11041 cttctagtat tttatatttg agaaataaaa tttaaccaaa atttgccaat ctagtaagct
    11101 tgtttttatt tgtatcttta aattttatga tagatgttat agtgtactct cctctcccct
    11161 cccctcccct ctcctcctca gcaggatctt gttatgtagc cctaagggat ctaatactta
    11221 ctctgtagtc caggctgtcc ttcagctcat gagacacacc atcatgcttg gcatgtttct
    11281 tgttctgaaa ggtatctggt tttaattttg gttgagctgg tgtgttggtg tatatctgta
    11341 atctcagcac aggcaagtag agacaaggat tatgattaca gtccagcctg ggctatttag
    11401 agggattctc tttaatcttt cctcatgccc caaaatctta cctttggtat atgaaaaata
    11461 gtctccactt ctgcaaaata ttgctttatg aagtaagaat taattccctt catagagaca
    11521 aggaatgtgt cctgtaaaag tttaatgtgt aagaagtatt tgttataaaa gataaatatt
    11581 cagaatcttc tttttaattc ctgattttat ttctatagga ctgaaagact tgctcgagat
    11641 gtcatgaagg agatgggagg ccatcacatt gtggccctct gtgtgctcaa ggggggctat
    11701 aagttctttg ctgacctgct ggattacatt aaagcactga atagaaatag tgatagatcc
    11761 attcctatga ctgtagattt tatcagactg aagagctact gtgtaagtat aattaactta
    11821 taattaaaaa aatagggcca ttctagtttt atctatattt tttttaaact tgtgcaaact
    11881 atgctacata atcaacttac ttgattatgt aatatatata gtaatagttg gaattactat
    11941 tattacatat ttatttttct gtgctggggt agaacccagg gaactaagta ctgtaataac
    12001 cgagcttcat tcatcctagt catttttaat tttttttttt ttttttgttt tttcgagaca
    12061 aggtttctct gtgtagccct ggctgtcctg gaactcactc tgtagaccag gctggcctcg
    12121 aactcagaaa tctgcctgcc tctgcctccc agtgctgaga ttaatgacgt gcaccaccac
    12181 tgcctggcat ttttttaaag ttttaatttg agaccctttc tcactaactt gctcacacta
    12241 tcctcgaact cattacgtgg gctggtctag aaatttcagt caacttgcct cagcttctca
    12301 agtagctggg attacaggca ggtgctacca cttctggcca tgatcattgt tgttgttgct
    12361 attattatta ttattatttc actgatactt catatcacaa cacagaatag taactgtagt
    12421 tcatgaccaa caaaaaagtt gtacagtttc tctgttgtct tattttgatc tggattttga
    12481 tttgtttcat ggcactgggc aggctaatct caaactgcat ggctcaaata gtccttctgt
    12541 cttaatctcc catgttctgg aagtataggt atcactacac ctagctgtat tttcatattt
    12601 taaaaaaaac gcctaagaat tgctattgaa tgaggcggag agatggctca gcagtcacaa
    12661 gtacttgtag aggactgggg tttggttact agcacctcaa agatggctta caattatctg
    12721 tagctccaat tccagaggat ctgtgtggta aagctgcatg tatatgggca tgcaggtaaa
    12781 actgctaaac atataaaaat agctcagtct taagagaatt ggtcgacttt ttcgcccttt
    12841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    12901 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    12961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
    13021 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    13081 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    13141 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    13201 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    13261 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    13321 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    13381 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    13441 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    13501 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    13561 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    13621 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    13681 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    13741 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    13801 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    13861 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    13921 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    13981 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    14041 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    14101 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    14161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    14221 ttgcaaacaa aaaaaccacc gctaccaggt cgagtaggga taacagggta atggatcctc
    14281 gttgggcacc tgcactcatg tacgcaggcc cacatataga tgtacacacg catatcattc
    14341 aaaatagaaa ttatttatac aagtagtgcg ttggagagct ggctcaggag ttgttaatgc
    14401 ttgctaccct tgcaaaggac ccaagttcaa tttccagcac ctgtgttggg catctcacaa
    14461 ccacatgtaa atctagtttc aggggatttc acccctcttc tgaactcttc aggcacttat
    14521 acccacaaat ggacatctcc acatagagac acatactttt aaaaaagtaa tctagggtct
    14581 agagaaatgg ttatagacca cggactggga tcagttccca gaagccacat cctgtggctc
    14641 acaattgcct gtgaatcaag ttctagatct ggtggcctcg tctagcctgc agtcatcaac
    14701 aaacacatgc acacatccat acacacataa tttaaaagaa agcgaaagta acctataatg
    14761 ctaaacaaag cacattctca gtgtagactg cagtggtgat gaaccttgaa aatatattaa
    14821 tagaagccag acacgtaagt cacaaatggt ataactcaat ttatatgaaa ttcccagaga
    14881 gtccaaagtt aaaaaaaaca aaaaggcaga ttattggttg tcagtagcta cagagatggg
    14941 acattgaaga tattaatagg tatagggttg aggattcatg gcctttccct cttccatgtt
    15001 agcatgtcta ttgttgttaa ccttattcaa ctcatgttag ggcaatcata ttgatgagac
    15061 ttaatgagta tagcttgaca ttcctagaag acagagtctc acatcagact ctctgatcct
    15121 ctgactctta gaatctttca acaccctctt ctgaaatgat ctctgagcct ttggttgtga
    15181 ctgaattatg agaaagaatt ttatgttgaa tgaattttac ttcagcattt ttacaaaact
    15241 gaagctgggg atggcgtttc atgcgtgcag tccctaccat ttgcctataa gcctggagga
    15301 ggcagaagta gaattaggct tttcagggac atcttcagct gcatcatgag cttgaggaca
    15361 acttgagcta cataagattc ccatccccac ccccacacac acacacacat ctagtatgta
    15421 cctagtatgc agtcttttca tatgtttaaa atgtagaaag gtatatgtga agtctccctt
    15481 ttccccacaa cttagtccct ccaaagtatt gcatcagttt ccttgtacat gattttatgc
    15541 atatataaga tacactattt ctctctctct ctctctcctt ccctccttcc ctccaacctc
    15601 tgggtatgag ttgagggtta tgtatgtttc aagttgtaat aagaattgcc actcaaacat
    15661 ttcatcccat tttaagttaa gtttgtctga ttttccactt tagaagggca gccatgatgg
    15721 ctcatgccta taatcccatc ctagcacttg taagcctaat acataagact ggaggattgt
    15781 catcagcttg aggctagcct ggtctaccta atgagttcca gccagccaag agtagaatat
    15841 aagaccctgt ctccaaaaaa tgtaccaaag gggctggagg ggtaactcag tggttaagaa
    15901 cagtagctgc tcttccagag gacctgggtt caattcccag cacccacatg gcagcttgca
    15961 actatctgta actgtccagc tttgggatat ccgacaccat tataccaatt acataaaatg
    16021 aaaaaattgt aaaatgtgct aaaatgagtg gggaggggga gcatggtgtg gttctgtggg
    16081 cttgggattt tgccatgaat ccatgactat atctcagcaa tatgtggatt atttctggaa
    16141 acctgtgggg catgggtgta tgggaagagg ggagagagag agagcccgca tccagccaga
    16201 gttcctgtgc tctgggcagg cggacgtggg aggactgctg gacgatttcc acttggccct
    16261 gggtgggcat ctggctgtgt gaagccactg accccactca gcggggggta gataaggggc
    16321 atttcccata ccaggttctc agactccagc attccacgct ggatacagca gaagagctga
    16381 gaacagaata ggggccccag ggagacactc tcgtcccaga gataaagaag gcagagggag
    16441 agagattcct acgcaggcga gaggccttag tctgggcctt gatgaacaga gacggtctat
    16501 ggttttagag ctttattgta gaaaggcagg gggaaagaga gaaggtagaa agagagagac
    16561 cagccatggc caagaggaga gaagggggga aaggagagag agaagaaagg ctagagagta
    16621 agaaaggtta aagagacaga agtaaaagct tagagtaaga gagagagaga gagcaagagg
    16681 gagagtaaga aaagtaaaag cttaagagag cgaggagggg ccagcccctc taatagtggg
    16741 cttgttatct tgttgttgct aggtaactgt gaagcgtcta gcctgaaggc cagaagcttg
    16801 ggacattgtc tatgtgactg aaagccatga gcctctactg tgggggctgt ggaggtggta
    16861 acttggacag gagccagggg ttccaggaga catgagggaa tgccttctgt cccatgtagg
    16921 taggaattac caccaccggc tttcaccgta gttcactgga gactgtttgt acatagccca
    16981 ttgccccaca gaaaccaggc actcactgtt tcatttgcat aagaattaga aggctttatt
    17041 ttggctggga ttggtgactt gccccagaaa ttccagcttt tgggaagccg aatcctggag
    17101 aaccaggagt tcaaggtcat cctcaggtac ctcatttgtt caagaccagc ctgtgctacc
    17161 atgagacctt gtccctactc ccctcaaccc caaaacacgc tgttttcatt ttataggttg
    17221 tactaaccat ggaggatggt catttgcctt tctcttgatt tcagaagcgt agcgcgggct
    17281 ttgcaccatg atctagtgtt tccagatagg cttgcctctc cacagccagg cttatcctgc
    17341 ttcagtccca tgtttggcaa gggcaacata tgactgaaga acttgcatag ccttatggtg
    17401 tatcatgact cttagcccca gtttataact gtctaacagc tggtggcttt tgtgaaaaag
    17461 gaaagcatca tttgaacttt taacttttca gctgtttgtt ttgactttgc ataaatactg
    17521 agactagcta ggaaatgagc ttttgtttca tttgctaaaa atgatttttt tttcatgagg
    17581 caatttcaag caatttgaat ttagcaatct ctacttgtct gaaaagaagt tattaattaa
    17641 aaaaaaacaa acaaacagga acctattatg ctggctagtc acatgaaaga atccaaatga
    17701 acacagtcaa ggctgaatca acacatgaaa ttattttcta attccccaaa ttacatttat
    17761 gctaattagt atgtagtaaa acattttaat tgtttccaat gttaaacatt acagaaataa
    17821 ttttgatgtt ttcctaataa cccagccttt gatcatgttt gcatttattt gattgttgtc
    17881 agtttattaa ataattaagt aagagcaaca agataaaatg acttcatgag agtccttttc
    17941 tgtaacttag aacgtcagta gtcataggaa ctgcggtcgt tcccttacct ttcagaagta
    18001 ggctaaagag ttgaacgcaa aggtggtttt tcgaatcttc ctgatcctta agcccccact
    18061 aggtttatat tctatatttg atatcggatt cgaacccctc gagcttagga tccggaaccc
    18121 ttaatataac taagttatta ggtccctcga agaggttcac tagtactggc cattgcggcc
    18181 gcttcgaggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    18241 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    18301 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    18361 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    18421 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
    18481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    18541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    18601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    18661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    18721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    18781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    18841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    18901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    18961 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    19021 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    19081 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    19141 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    19201 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    19261 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    19321 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    19381 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    19441 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    19501 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    19561 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    19621 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    19681 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
    19741 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    19801 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    19861 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    19921 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    19981 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
    20041 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    20101 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    20161 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    20221 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    20281 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    20341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    20401 ccacctgacg g
//
