LOCUS       sequence-135543-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Fujita T, Kitaura F, Fujii H
  TITLE     A Critical Role of the Thy28-MYH9 Axis in B Cell-Specific Expression
            of the Pax5 Gene in Chicken B Cells.
  JOURNAL   PLoS One. 2015 Jan 21;10(1):e0116579. doi: 
            10.1371/journal.pone.0116579. eCollection 2015.
  PUBMED    25607658
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-21"; volume: "10(1):e0116579. doi"; pages: " 
            10.1371/journal.pone.0116579. eCollection 2015"
COMMENT     Sequence Label: 3xFNLDD/pMXs-I2
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             114..179
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             216..236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(249..500,501..857)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     792..813
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 gctgccgacc ccgggggtgg accatcctct agactgccgg atctagctag ttaattaagg
       61 atcccagtgt ggtggtacgg gaatcgttta gtgaaccgtc agaattaacc atggactaca
      121 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagc
      181 ttgcggccgc gaattcatcg atagatcgat cccctccaaa aaagaagaga aaggtcgaac
      241 ctgagggaat gaaagcgtta acggccaggc aacaagaggt gtttgatctc atccgtgatc
      301 acatcagcca gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc
      361 gttccccaaa cgcggctgaa gaacatctga aggcgctggc acgcaaaggc gttattgaaa
      421 ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga agaggaagaa gggttgccgc
      481 tggtaggtcg tgtggctgcc ggtgaaccac ttctggcgca acagcatatt gaaggtcatt
      541 atcaggtcga tccttcctta ttcaagccga atgctgattt cctgctgcgc gtcagcggga
      601 tgtcgatgaa agatatcggc attatggatg gtgacttgct ggcagtgcat aaaactcagg
      661 atgtacgtaa cggtcaggtc gttgtcgcac gtattgatga cgaggttacc gttaagcgcc
      721 tgaaaaaaca gggcaataaa gtcgaactgt tgccagaaaa tagcgagttt aaaccaattg
      781 tcgtagatct tcgtcagcag agcttcacca ttgaagggct ggcggttggg gttattcgca
      841 acggcgactg gctgtagtcg ag
//
