LOCUS       sequence-135564-        6738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the S. Lycopersicum fruit promoter NH as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pNH  (GB0064)
FEATURES             Location/Qualifiers
     source          1..6738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3788..3806)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3789..3806)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3824..3840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3824..3840)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3837..3859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3848..3864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3872..3878,3879..3896,3897..3902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3917..3938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4055..4072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4226..4814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4306..4325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4985..5776,5777..5845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5608..5627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5846..5950)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6028..6483)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6165..6186)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6377..6396
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6609..6631
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6623..6640
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6624..6640
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6647..6666
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6647..6665
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6696..6705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6700..6709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgggagat catttttcaa cgaaaaaggt taattagaat cccttaactc tgcttgactt
       61 cgctatcgct cgttcatttg taagaatatc gtcaaacatc attcgtctgt acgggaatta
      121 tttattttct taaaaagaaa aattctataa cattatatat atataatttt taggatagcc
      181 taacgttaca tgtaaagtta tagtcacgtg atcaaaaagt catcataaag gataaatata
      241 tttccgagtt atcttaaatg gtatgaagat actctccgtc atacttttga gacattggag
      301 cccctgtcgt tcaaaaacta gagcaaatat gtccttcatt ctaaaagaag actaaatagg
      361 aaaacgtgac ataatcttat ccgttgatcc gatatttaat aacggtcata tcggtggata
      421 aaattgcgac acgtgtatgt ccattagtat aaaaggtata tatgctctaa tttttaaacg
      481 acagggacaa caatatctca aaagtatgac gaaggatgta tctacatacc atttcttata
      541 attcggagta tatttgtctt gtttctcttt aactataaag aacaacctcg tacaaaaata
      601 taagcgctat tgacttaatt ttcctccaaa cattgcgtat aactaaagct tagtacatcc
      661 gactgtcttt cggtcaaact acatacatat aagataaaga gcatgggcna atgacacaaa
      721 tccagcgtgg caaaatcaga gaaagtaaat gaaaatgggg cccnaatagc acgagtagta
      781 ctacctggan aagcatccaa agggtcttaa tangggccca ctaggcccaa atnaagtagg
      841 gcccatttag agaaagccca aaaggattta gatctaaaaa ggaagtgcca cgtcagaata
      901 tggggcccat ttagacgtna atggtcgtaa attgngatag tgggggccca tttagcaccg
      961 gcccttcact attaatttgg actttacgtt cataaccaan ttgtccacaa atcnaaaata
     1021 ntaataataa ttcaacttgc cttttatttt ttcttcaaat tcctaaacat tctcatcttg
     1081 actagtattg tagaaaaaaa cattttacta tttttggtga aattttgatg tgattttact
     1141 ctaatgtaat tttactgttg ttaatgaaaa ggtgtgtcat gccgacaata aataaataaa
     1201 aagataattc aatgtaaaaa ttatttcata tttaacgaga tctgaaagga tcgtcatatt
     1261 tcaaaaagaa gtaatgtagg tagtttatct tcagcatgta ttaatgtctg atcatgaccc
     1321 gaattcgtga ttaatagatc gatccaatcc aaaggcggca acagaaaatg tataatcaaa
     1381 atagtaatat cacgagttta agttgtttaa tatttttttt atttaattta tacgatgcga
     1441 agaggtatgg agcttttgat ttaactataa tataatattg gtttggttat aaaacttttg
     1501 aaatacacga cttaaaccta tgaaatattt aataagttaa attattttta gattgtaaca
     1561 tattttataa tttttgcaaa taaaacaacc tattaaaatg aatttaacta aaaaatatat
     1621 atcataccac attgataaat gcacacatag ttaaaagaaa atatgtttca actaattaaa
     1681 catttataca ataatttgcg agatccaata cataacatta aaaaaaatag ataaaaaggt
     1741 tagagtcatt atttacatgg attagcaact taattcattc gagcgataaa agttgtgata
     1801 gaatgataaa tatttttttg atttttttaa atttaaatag aaatttcaaa ttcaaacgtt
     1861 agttacgaaa gcatgattgt tacagaatat atatattaag tataactttt cgatatgaat
     1921 caaaattcaa tcgaacccta ctgaacgttg gatgggaaat tttaaaaaat taaattaatt
     1981 tccttaaacc aaggagattt tgttgtaaag gaactaaaat taagaaaaag attaattttt
     2041 aaaaattaga agaacatgaa agcatgatgg tcttagtcac ctacatgccc tactgcacaa
     2101 aacacagaaa gaaaaaagat ataatagctg ttatagtaaa tggtcaccac caccatgtgg
     2161 cattgctatt tcccaatcaa aacaagtgct tttagacaaa cctaataaat gaatttatta
     2221 attacatttt ttttaaaaaa aaaattgtgt ttgagtcgaa gatctatcga atcacatatg
     2281 tttagttatc ctttaaggta aaaatgagat ttcatgtaca cactattctt ttcacttgcg
     2341 gcatgagact aactatgtta ttgttactgt attattacgt agaaaatgcc taattttgaa
     2401 aatcaataag gaaaaaataa aaataatgtg ttgaatatta ttttcttaga aaatattatc
     2461 gatgtgaatt tattcatatt ggtggtccta aatttgaatg aaaagattaa taagtaatta
     2521 aatgaatatg taatgtcacc aaacggaaag gtttttattt tcaaaataaa taaggaagca
     2581 aaaaggaaaa gaatgtgtta agagtgtttt tcttataaga gattgttaat gtgaactgca
     2641 tttatattgt cgatttaatg tttgaataaa taaatttata atcttgtcaa agaaaaaact
     2701 cttcatattg aaaatcaaaa aggaaaaaaa atgtgtaaga atattatttt tcttaaattt
     2761 attcatatta tcaatctcaa gttcaaatta ataaataaat taatttataa tgccatgaaa
     2821 gaaaaaaatt cgccacgttg aaaaacaata aagaagagag gggaaagagg atacaatttt
     2881 gtttattatt aactagattt tcgttttgtg attgttgaaa ttttaactgt tttattcata
     2941 agtcttaaat tcgaactata tgaaattttt ttaaaaaaat cctcaataaa aagtgtttta
     3001 cttataaata ccatctaatg ttaagactat atatttagaa aatgaaagtt tcaaatttca
     3061 aattttactt gttaaatttt aaattctata ataatagcta aaaaaatgga tatttgtgaa
     3121 ttttactatg tgttacttga tggacttact ccattagaat ctagactaat cgaatgtgta
     3181 aatttaaata tccgataatt ataacaataa caacagactg aatattattc catatatata
     3241 tatatagaga gagagagaga gagagagaga gaaatcttaa ttctatctaa aggtaatttt
     3301 taatagatcc tcaactcgcg tatgaattga ttaaacttat attatataag gtaaggaaaa
     3361 aaggaaatat tttgtatgat tactattatt ttcttattat tataaacata aagtaaagta
     3421 aaagtaaata catttacaac tagacatcca aatcttttgt acacccttaa ttgtcagtga
     3481 tatgaggtgt caatatgtat atacttgaag attctttttt tcttccatta attaatttta
     3541 tattaataca ctccatttta ctttctctat ataaagctct ctcatttgat cattttttcc
     3601 atcaacaaat tacaattaca aatattttgc ccccttccat ttcttaattt attttcctct
     3661 ttcccatctt cttattttcc aacttaatgc gagaccactc atcgcccaat cactagtgaa
     3721 ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct
     3781 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
     3841 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3901 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3961 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4021 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4081 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5101 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5821 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5881 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5941 gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga
     6001 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     6061 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     6121 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6181 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6241 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6301 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6361 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6421 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6481 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6541 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6601 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     6661 atagggcgaa ttgggcccga cgtcgcatgc tcccggccgc catggcggcc gcgggaattc
     6721 gattgggcga tgagtggt
//
