LOCUS       sequence-135567-        5059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the S. Lycopersicum translationally-controlled tumor 
            protein homolog promoter as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPTctp  (GB0083)
FEATURES             Location/Qualifiers
     source          1..5059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2109..2127)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2110..2127)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2145..2161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2145..2161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2158..2180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2169..2185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2193..2199,2200..2217,2218..2223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2238..2259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2376..2393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2547..3135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2627..2646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3306..4097,4098..4166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3929..3948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4167..4271)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4349..4804)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4486..4507)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4698..4717
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4930..4952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4944..4961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4945..4961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4968..4987
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4968..4986
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5017..5026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5021..5030
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcattgg tggaagagta gcctttttct ttctctctct aaagttaggg ttgattggaa
       61 gagagaggaa gaaaacgacg caccagtgaa ggctcgagaa gaagggtttt ggctggagta
      121 cggtgtcttt atagcactga gcacttgtaa attaccaaaa taacctgcct tgaatccaga
      181 attacgtatt tgcctacccc aatttgagta gggttctgaa tgccgccgca ctctgcccca
      241 tgtctcaact agtgcaaagg ccatattact tactttacta atcctaatag aatcaaaata
      301 tcctcgtcca cgctagggta aaaaataaat tttaaatgtt gtgtaattat aaattattaa
      361 ataagaaaat aaaatatatt tttttaaaaa aaaaaattat atctctaata gcatagaagt
      421 gacatttgtt tgagaagatt aaagttaaaa gcgagtcacg tgaattagaa tgaagcgagt
      481 aataatcaat aaatttatat agttttataa gaagttattt taaattttaa atatgaaaaa
      541 aataaaaaga atatatctgc caaattgaat tttacacgtg atgaactcaa aaattcttat
      601 ctaggaaatt atttttctga atgaatctta cacgatgtaa atttaaatta atcgaatttc
      661 aattcaagtg ctgaacatag tctgagaaaa gaataggaaa acgttgtcaa aagataaata
      721 ttgacccact tgttaaatct ttgatgctag tatataatca aattttggca atgttggaaa
      781 aatctataac tcttgaaaac catatttatt cattttttta ttttttttta cgtttaagaa
      841 tagttttaaa tggacaattt gattccttca tcaaaacata tttcctcttt tgttatttat
      901 ttttgttctt ttaagaggat cgcctatcca aaatttgcta ctaactctaa ttattaattc
      961 accatcaaat attaagttca tcttacttaa agctagttat tatgatattg ataatcgtat
     1021 ataatagcaa attaataaca taaaataaat gaaatagcta cggtttgatt taattgtact
     1081 tcataacaaa cattagccaa agtttgtcag tcgcctctct cccaaaaatc tctcgccact
     1141 ctcccattct cgctcgccac tttcccattg ctcgtgtctc tcgctttata tacagaagtg
     1201 tataaattgt gtttttgttt tgtcgagaga aaattgtata tacacatgca aaaacatata
     1261 tctttgtgct atatacttaa ttatacaatt tacaaacatt ttacttcatt taaattgtaa
     1321 acaaattcaa attttataca aatattgcag cgagaaaggt caacgaatta tacaattgca
     1381 gtgaaataca attttctctc gctttataca acagaagtgt atatattgtg tttttgtttt
     1441 tgtataaagc gagagaaaaa catatatctt cttcctatac acttataatt atgcaatata
     1501 catacatttt acttcgattc aattgtatgc aaaacaaatt ttatacaaat attgtagcga
     1561 aataggcaac gaattataca attgtgcatt atacaattac agggcagcga attatacaat
     1621 ttaggtcagc gaattataca atttaggcca gcgaattata caattgtaaa ttttatagtt
     1681 ttatatttgc tatggagcgc aattatgcaa attttgctat aacatacaaa tatgattttt
     1741 ttgtttggtg aaagttgtcc attacaatag gactagtttc ttcttttaat gtacatgcat
     1801 aactccgaca acaatatact acagtttaag atatattagt aatagctggg tgtttaactg
     1861 attttttcac tttctaagat cttattgaaa tagctctacg ctgaaattat ttgaaatgaa
     1921 ataagatttg atgttttaat aatttgaaat gaaataacat ttgaggttct ctttctttct
     1981 gaaattcccg acttcacaat attttactcc cgagaccact catcgcccaa tcactagtga
     2041 attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg gatgcatagc
     2101 ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct
     2161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3061 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3121 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3181 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3241 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3301 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3361 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3421 ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga tttatcagca
     3481 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3541 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3601 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3661 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3721 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3781 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3841 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3901 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3961 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4021 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4081 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4141 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4201 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4261 ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag
     4321 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     4381 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4441 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4501 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4561 gctggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4621 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4681 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4741 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4801 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     4861 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     4921 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
     4981 tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt
     5041 cgattgggcg atgagtggt
//
