LOCUS       sequence-135570-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcctcttct tcttggggtc agccctgctg tctccaccga gctgagagag gtcgattctt
       61 gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta
      121 gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc
      181 aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat gggcttatcc
      241 ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag gatcactctt
      301 ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg ttgttccacg
      361 aacagctgct tctgctcatt atcttcggga gaccctttga gcttttcata gtggctggcc
      421 agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt ctgcagctcg
      481 cccgcactag cgagcattcg tttccggccg ttttcaagct caaagagaga gtacttggga
      541 agcttaatga tgaggtcttt tttgacctct ttatatcctt tcgcctcgag aaagtcgatg
      601 gggttttttt cgaagcttga tcgctccatg attgtgatgc ccagcagttc cttgacgctt
      661 ttgagttttt tagacttccc tttctccact ttggccacaa ccagtacact gtaagcgact
      721 gtaggagaat
//
