LOCUS       sequence-135579-        4992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the CDS of the Antirrhinum majus Delila transcription 
            factor as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDelila (GB0079)
FEATURES             Location/Qualifiers
     source          1..4992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2042..2060)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2043..2060)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2078..2094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2078..2094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2091..2113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2102..2118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2126..2132,2133..2150,2151..2156))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2171..2192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2309..2326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2480..3068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2560..2579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3239..4030,4031..4099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3862..3881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4100..4204)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4282..4737)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4419..4440)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4631..4650
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4863..4885
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4877..4894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4878..4894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4901..4920
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4901..4919
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4950..4959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4954..4963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgaagctc aagacttcat agtaactttc tgaagagctt gtttgatcac acttgctgat
       61 gcaaccttca atcccttgca cttggctttt atggtaatgg atataattcc atctctgttg
      121 gaagattgaa cagtttcgga atccaaactt agtcgtctta cggcttccat cacttcaagc
      181 aatacaaact ccttggaaga acaactcacg acaatcaaca catccttgtt tgtaatgttc
      241 acagttatat tatctgttaa ggaatctttc aatcgacctc tgctatttac ggctccaatc
      301 ttgtccgtat cagaagcctt tctcttgttt gtcaacggtt tcttgacatt acttgtcctt
      361 gttgtgccat aattatcgga ggtcctctca atggcatcgt gcagtttagt ttttgtagtt
      421 gattcccgcc cccggccctt taccattttg ttagattcca gctcgtcgac tttcctctca
      481 agccctctca agtaatctat tgtatggtct agtattgata ctttgtcaac cttgccaccg
      541 gatgggacta gggatgcaag aatcataaac cgttcgttta ttttctctct gcgttttctc
      601 tctgacaaga cgtggtttct atcaatttca tcagccgttg gctttgcaag gcagtcactg
      661 ttgccttttt gtttaccagc atcaagcctg gagttttcat gcattctagc tacttcaaaa
      721 agtactttct tcagaaatct ttgtgaggtt ccgcttcggg gaacatgagt acccgacgat
      781 ccatccttgt tccaactaac gaagcttgat tctctattcc catttctgaa gtagggacca
      841 agaaccaact gatgggaact cttcaaaaga ttggaaagta ccccttgata atggacctcg
      901 tcgccttgga cacccgtgtt ttctatctgc tgattgcatt tttgagtgct gtgcatacaa
      961 ttatttgtct ccggtgcccc ttttccatca gaaagtggag caaaagactc tagattttca
     1021 tgagtttgag atatacagtc actagaattc atagaactat tgagacaatt gctgattgca
     1081 tcatccatga aaggccagct ttgtgtttga ggaacctccc cattgatgcc ttctgccaaa
     1141 ttcgattcgt ctatgagtaa attgtctgca aagtcatcca aactgttatc aggagaacat
     1201 atgttcgtgt ctggacaatt caaaagctga tcaagatcgt tttctggtat attagcatgt
     1261 tcaagcgctt cacaaatgag gtcattgttg tttgtaatac tgttggagac atagttggga
     1321 atcttgggaa cggtggcagg actgtccaag aatgaagttt ttatatgctg gattagattc
     1381 aaatcctccg gtactagctc tgttgctccc agctcaacta ctccttctgc atatggaaag
     1441 cacacaactg tctgaattga cgcactcttt gcaagcaaag aacgcgagaa aactttggtg
     1501 tccgcacgat gagcgttgca tagccatact gcttgatttc gtgctaatgt tcttccaggc
     1561 aacccttggc caatattgaa tatgaaagac atgcaaacca agaaaaacca ctcagcatca
     1621 gtgaggtctt ctggtgataa tgcagcagta ggccttttag cttgtgtgtt ggtttcacca
     1681 agtgaaagag actcataaag ttctctcaat tgatcacttc tctgcaatcc cagctgatcg
     1741 ggattcaatt cgacagattg tacagttttt cgagttttaa tatctccatt gtagaaccca
     1801 tcaccccact ccaagacccc tggttgtgca actgaattgg accagaaaat tgcataactc
     1861 cattggatac ttctaacagc aatagcaagt tgcttcctca aattctcagg cactatcttt
     1921 tggttttgga taccagtagc cattcgagac cactcatcgc ccaatcacta gtgaattcgc
     1981 ggccgcctgc aggtcgacca tatgggagag ctcccacgcg ttggatgcat agcttgagta
     2041 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2161 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2281 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3361 gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc
     3421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4201 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     4261 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4321 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4381 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4441 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgctggcc
     4501 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4561 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4621 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4681 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     4741 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     4801 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     4861 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
     4921 cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattgg
     4981 gcgatgagtg gt
//
