LOCUS       sequence-135591-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Fujita T, Kitaura F, Fujii H
  TITLE     A Critical Role of the Thy28-MYH9 Axis in B Cell-Specific Expression
            of the Pax5 Gene in Chicken B Cells.
  JOURNAL   PLoS One. 2015 Jan 21;10(1):e0116579. doi: 
            10.1371/journal.pone.0116579. eCollection 2015.
  PUBMED    25607658
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-21"; volume: "10(1):e0116579. doi"; pages: " 
            10.1371/journal.pone.0116579. eCollection 2015"
COMMENT     Sequence Label: 3xFNLDD/pMXs-IG
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             125..190
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             227..247
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(260..511,512..868)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     803..824
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatctagct agttaattaa
       61 ggatcccagt gtggtggtac gggaattcct gcaggcgttt agtgaaccgt cagaattaac
      121 catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
      181 cgatgacaag cttgcggccg cgaattcatc gatagatcga tcccctccaa aaaagaagag
      241 aaaggtcgaa cctgagggaa tgaaagcgtt aacggccagg caacaagagg tgtttgatct
      301 catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg
      361 tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg cacgcaaagg
      421 cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga
      481 agggttgccg ctggtaggtc gtgtggctgc cggtgaacca cttctggcgc aacagcatat
      541 tgaaggtcat tatcaggtcg atccttcctt attcaagccg aatgctgatt tcctgctgcg
      601 cgtcagcggg atgtcgatga aagatatcgg cattatggat ggtgacttgc tggcagtgca
      661 taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg acgaggttac
      721 cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa atagcgagtt
      781 taaaccaatt gtcgtagatc ttcgtcagca gagcttcacc attgaagggc tggcggttgg
      841 ggttattcgc aacggcgact ggctgtagtc gacctcgagg gccggcgcgc cgcggccgct
      901 acgtaaa
//
