LOCUS       sequence-135603-       10328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinD1 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnd1-AP-His
FEATURES             Location/Qualifiers
     source          1..10328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6908..6925
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6951..7072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6988..7007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7042..7061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7110..7127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7281..7869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7361..7380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8040..8831,8832..8900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8663..8682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8901..9005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9073..9091
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9129..9151)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9153..9173)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9158..9488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9339..9474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9401..9420
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9625..9644
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9711..10166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9848..9869)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10060..10079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10292..10314
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10306..10323
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10307..10323
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggctcgtcg
     1621 cgccgcgggc ggcgcacccc ctagcgcccg ggccgccgcg gccgtcccct tgcgtccgcg
     1681 ccctcactcg cggggccctg gtctgctgcc gctgcctctg ctgctgctgc tcggggcggc
     1741 acgggccggc gccctagaga tccagcgccg tttcccctcg cccacgccca ccaacaactt
     1801 cgccctggac ggcacggcgg gcaccgtgta cttggcggca gtgaaccgcc tgtaccaact
     1861 gtcgagtgcc aacttgagcc tggaagccga ggcgaccgtg ggtcccgtgc cggacagccc
     1921 gctgtgtcac gccccgcagc tcccgcaggc ctcgtgcgag cacccgcggc gcctcacgga
     1981 caactacaac aaaatcctgc agttggaccc gggccagggt ctggtggtcg cgtgcggctc
     2041 catctaccag ggtctgtgcc agctgaggcg ccggggcaac atctcagccc tggccgtgag
     2101 ctttccgcct gccgcgccga ccgcagaacc ggtcaccgtg ttccccagca tgctcaacgt
     2161 ggccgccaac caccccaacg cgtccactgt gggactggtg ctgccgccta cctcgggcac
     2221 cgggggcagc cgtctgctcg tgggcgccac gtacaccggc ttcggcagcg ctttcttccc
     2281 gcgcaaccgt agcctagaag accaccgctt cgagaacacg cccgagatcg ctatccgctc
     2341 cctggacgcg cgtggagact tggccaagct cttcaccttc gaccttaacc cgtcggacga
     2401 taacatcctg aagatcaagc agggcgccaa ggagcagcac aagctgggct tcgtgcgtgc
     2461 cttcttgcac ccggcggtgc caccgcacag cgcgcagccc tacgcgtacc tggcgctcaa
     2521 cagcgaggcg cgtgcgggcg acaaggacag ccaggcgcgc agcctgctgg cgcgcatctg
     2581 cctgccccgc ggcgcgggtg gcgacgccaa gaagctcacc gagtcctaca tccaactggg
     2641 cttgcagtgc gcgggcggcg cgggccgcgg cgacctctac agccgcctcg tgtcggtttt
     2701 ccccgcgcgc gagcagttct tcgctgtctt cgagaggccc cagggcgccc caggtgcccg
     2761 caacgccccg gccgcgcttt gcgccttccg cttcgacgac gtgcaggctg ccattcgtgc
     2821 agcgcgcacc gcctgcttcg tggagccggc gcccgacgtg gtggcggtgt tggacagtgt
     2881 ggtgcagggc accgggccgg cctgcgagag caagcgcaac atacagctgc agccggagca
     2941 actggattgc ggagcggccc acctgcagca cccactgacc atcctgcagc cgctgagggc
     3001 atcgcccgtg ttccgtgctc cagggctcac ggccgtggct gtggccagtg ccaacaacta
     3061 cacggccgtc tttctgggca ccgccacagg gaggctcctc aagatcagcc tgaacgagag
     3121 catgcaggta gtaagcaggc gagtgctgac tgtagcctat ggggagcctg tgcatcacgt
     3181 catgcagttt gaccccatgg atcctggtta cctatacctg atgacatccc accagatggc
     3241 ccgagtgaag gtggcagcgt gtgaggtaca ctccacctgc ggggactgcg tgggtgcggc
     3301 cgatgcctac tgtggttggt gcactctgga gacccggtgc acactccagc aggattgcac
     3361 caactccagc cagccacatt tctggaccag tgccagtgag ggccccagcc gctgccctgc
     3421 catgacagta ctgccctcgg agattgatgt gcaccgggac tacacaggta tgatcttaca
     3481 gatctcagga agcctgccca gcctcagcgg catggagatg gcttgtgact atggaaatgg
     3541 cgttcgaacg gtggcccggg tacctggccc tgcctatgat catcagattg cctactgcaa
     3601 tctcctgccc agggcccagt ttccatcctt tcctgctggc caggaccacg tgaccgttga
     3661 gatgtctgta agggtcaaag gacacaacat tgtctcagcc aatttcacca tctacgactg
     3721 cagccgaatt ggacaagtct acccccatac agcctgtacc agctgcctgt ccacacagtg
     3781 gccttgctcc tggtgcatcc agctgcattc atgtgtctcc aaccagtctc agtgccagga
     3841 ctcgccaaac cccacgagtc ctcaggactg tccccagatc ctgccctcgc ccctagcgcc
     3901 cgtgcccaca ggtggctccc aagacatcct ggtgcccctg actaaagcca ccttcttcca
     3961 tggttcctcc ctcgagtgca gctttgggct ggaagagagc tttgaggctg tatgggcgaa
     4021 taactcactg gtccgctgca accaagtggt gctgcacaca acccagaaga gccaggtatt
     4081 tccactgagt ctgaagctga aggggccgcc agaccgattc ctagacagcc ctaaccccat
     4141 gacagttgtg gtctacaact gtgctatggg cagccctgac tgttcccagt gcctgggccg
     4201 tgaggacctg ggtcacctct gtgtttggaa tgatggctgt cgtctaagag ggcccctgca
     4261 gccactccct ggcacctgcc cagcccctga aatccgagcg attgagcctc tgagtggccc
     4321 cttggacggt gggactttgc tgaccatccg tggcaggaac ttgggccgtc ggctcagtga
     4381 tgtggcacat ggtgtgtgga ttggcagtgt ggcctgtgaa cccctggctg acagatacac
     4441 cgtttcagag gagatcgtgt gtgccacagg gcctgccgca ggggccttct cagacgtggt
     4501 aacggtgaac gtctccaaag aaggcaggtc tcgggaacag ttctcctatg tgctgcccac
     4561 agtccactca ctggagcctt ccatgggccc aaaggccggg ggtacaagga tcaccattca
     4621 cggcagtgac ctcaacgtgg gctctatgct ccaggtcctg gtgaatgaca cggacccctg
     4681 cacagatctt acgcgcacag ccaccagcat cacctgcact gtgccagggg gtaccctgcc
     4741 ctctccagtg cctgtgtgtg tgcgcttcga gagccggggc tgcgtgcacg gaaacctcac
     4801 cttctggtac atgcagaacc cagtcatcac agccatcagc ccaggccgca gccctgtcag
     4861 tggcggcagg accatcactg tggctggcga acgcttccac atggtgcaga atgtatcaat
     4921 ggctgtacac cacattggcc gggagcccac gttctgcaag gttctcaact ccacactcat
     4981 cacctgccca tctcctggag ccctgagcaa tgcttcggcg cctgtagact tcttcatcaa
     5041 tggccgggca tatgcagacg aggcagccga ggagctgctg gaccctgcag aggcacagag
     5101 gggcagccgg ttccgcctag actacctccc caacccacag ttctccacag ccaagaggga
     5161 gaagtggatc aaacatcacc caggagagcc gctcaccctc gtcatccata aggagcaaga
     5221 cagcctgggg ctggagagcc atgagtacca catcaagatt ggccaggtgt cctgcgacat
     5281 ccaaatcatc tcagacagag tcatccactg ctcagtcaat gagtcgctgg gcacggctga
     5341 aggacagctg cccatcacaa tccaggtggg gaacttcaac cagaccatcg ccacactgca
     5401 actggggggc agcgagacgg gcgcgcctgg atcttccgga atcatcccag ttgaggagga
     5461 gaacccggac ttctggaacc gcgaggcagc cgaggccctg ggtgccgcca agaagctgca
     5521 gcctgcacag acagccgcca agaacctcat catcttcctg ggcgatggga tgggggtgtc
     5581 tacggtgaca gctgccagga tcctaaaagg gcagaagaag gacaaactgg ggcctgagat
     5641 acccctggcc atggaccgct tcccatatgt ggctctgtcc aagacataca atgtagacaa
     5701 acatgtgcca gacagtggag ccacagccac ggcctacctg tgcggggtca agggcaactt
     5761 ccagaccatt ggcttgagtg cagccgcccg ctttaaccag tgcaacacga cacgcggcaa
     5821 cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg aagtcagtgg gagtggtaac
     5881 caccacacga gtgcagcacg cctcgccagc cggcacctac gcccacacgg tgaaccgcaa
     5941 ctggtactcg gacgccgacg tgcctgcctc ggcccgccag gaggggtgcc aggacatcgc
     6001 tacgcagctc atctccaaca tggacattga cgtgatccta ggtgggggcc gaaagtacat
     6061 gtttcgcatg ggaaccccag accctgagta cccagatgac tacagccaag gtgggaccag
     6121 gctggacggg aagaatctgg tgcaggaatg gctggcgaag cgccagggtg cccggtatgt
     6181 gtggaaccgc actgagctca tgcaggcttc cctggacccg tctgtgaccc atctcatggg
     6241 tctctttgag cctggagaca tgaaatacga gatccaccga gactccacac tggacccctc
     6301 cctgatggag atgacagagg ctgccctgcg cctgctgagc aggaaccccc gcggcttctt
     6361 cctcttcgtg gagggtggtc gcatcgacca tggtcatcat gaaagcaggg cttaccgggc
     6421 actgactgag acgatcatgt tcgacgacgc cattgagagg gcgggccagc tcaccagcga
     6481 ggaggacacg ctgagcctcg tcactgccga ccactcccac gtcttctcct tcggaggcta
     6541 ccccctgcga gggagctcca tcttcgggct ggcccctggc aaggcccggg acaggaaggc
     6601 ctacacggtc ctcctatacg gaaacggtcc aggctatgtg ctcaaggacg gcgcccggcc
     6661 ggatgttacc gagagcgaga gcgggagccc cgagtatcgg cagcagtcag cagtgcccct
     6721 ggacgaagag acccacgcag gcgaggacgt ggcggtgttc gcgcgcggcc cgcaggcgca
     6781 cctggttcac ggcgtgcagg agcagacctt catagcgcac gtcatggcct tcgccgcctg
     6841 cctggagccc tacaccgcct gcgacctggc gccccccgcc ggcaccaccg acgccgcgca
     6901 cccgggtcat catcaccatc accattaatc tagagatctt attaaagcag aacttgttta
     6961 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7021 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7081 ggtcgactct agctagactc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7141 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7201 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7261 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7321 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7381 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7441 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7501 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7561 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7621 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7681 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7741 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7801 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7861 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7921 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7981 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8041 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8101 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8161 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8221 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8281 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8341 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8401 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8461 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8521 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8581 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8641 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8701 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8761 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8821 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8881 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8941 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9001 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     9061 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     9121 ggtgaaaacc tctgacacat gcagctcccg gaggaatgtg tgtcagttag ggtgtggaaa
     9181 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9241 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9301 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9361 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9421 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9481 tttgcaaaaa gctcccggga gcttgtatat ccattttcgt cccggagacg gtcacagctt
     9541 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9601 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9661 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag
     9721 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     9781 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     9841 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     9901 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     9961 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
    10021 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
    10081 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
    10141 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa
    10201 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    10261 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    10321 agtgaatt
//
