LOCUS       sequence-135607-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid 
            #8452) with GFP instead of puromycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMKO.1 GFP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..306
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     58..78
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     229..248
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(362..382)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        367..696
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      547..682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(705..721)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(706..722)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattga agatctgtcg acaaggtcgg gcaggaagag
       61 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      121 attagaatta atttgactgc aaacacaaag atattagtac aaaatacgtg acgtagaaag
      181 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      241 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      301 aacaccggta cgcgtactag tactcccata tggaattcgc cagcacagtg gtcgaccctg
      361 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      421 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
      481 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
      541 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
      601 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
      661 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttatcgatac cgtcgacctc
      721 gagaccatgg tgagcaagca gatcctgaag aacaccggcc tgcaggagat catgagcttc
      781 aaggtgaacc tggagggcgt ggtgaacaac cacgtgttca ccatggaggg ctgcggcaag
      841 ggcaacatcc tgtttcggca accagct
//
