LOCUS       sequence-135612-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, 
            Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV V5-LUC Blast (w567-1)
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             134..175
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      245..254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(317..335)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
       61 gactctagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagcagg
      121 ctccaccatg gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgggatc
      181 catgggaacc aattcagtcc ctaggctttt gcaaaaagct tggcattccg gtactgttgg
      241 taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      301 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      361 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      421 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      481 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      541 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
      601 tatggg
//
