LOCUS       sequence-135618-       10223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinA4 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxna4-AP-His
FEATURES             Location/Qualifiers
     source          1..10223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6803..6820
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6846..6967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6883..6902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6937..6956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7005..7022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7176..7764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7256..7275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7935..8726,8727..8795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8558..8577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8796..8900)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8968..8986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9024..9046)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9048..9068)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9053..9383
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9234..9369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9296..9315
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9520..9539
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9606..10061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9743..9764)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9955..9974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10187..10209
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10201..10218
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10202..10218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgaaagccat
     1621 gccctggaac tggacttgcc tgctctccca cctgctggtg gtagggatgg gctcctccac
     1681 tctgcttcca cgacagccac cccagctgtc tcagaagcct tcctttgtga cattccgagg
     1741 ggagcctgct gagggcttca atcaccttgt ggtggatgag aggacagggc acatttattt
     1801 gggggctgtc aacagaatct acaaactctc tagtgacctc aaagtcttgg tgactcatca
     1861 gacagggcca gatgaggaca accccaagtg ttacccacct cggattgttc agacctgcaa
     1921 tgagcctctg gccagcacca ataacgtcaa caagatgctg cttatagact acaaagaaaa
     1981 caggctgatc gcgtgtggga gcctgtacca gggtatctgc aagctcctga ggctagagga
     2041 cctcttcaag ttgggagagc cctttcacaa gaaggaacat tatctctcgg gggttaacga
     2101 gagtggctca gtctttgggg tgattgtttc ctacagcaac tttgatgata aactctttat
     2161 tgccacagca gtggatggca aacctgaata ttttcctacc atctccagca ggaagctgac
     2221 taagaactct gaggccgatg gcatgtttgc ttacgttttc catgatgaat ttgtggcctc
     2281 tatgattaag atcccttcag acactttcac ggttatcccc gactttgaca tctactacgt
     2341 ctatggcttc agcagtggca attttgtcta ctttttgacc cttcagccag agatggtgtc
     2401 tcccccaggc tccacgacaa aggagcaggt gtacacctca aagcttgtga ggctttgcaa
     2461 agaggacaca gcctttaact cctacgtaga ggtgcccatt ggctgtgagc gcaatggggt
     2521 ggagtatcgc ttgttacagg ctgcctactt gtctaaagct ggtgcagtgc ttggccggac
     2581 cctaggagtc cgcccagatg acgacctcct cttcaccgtc ttctccaagg gccagaagcg
     2641 gaagatgaaa tctctggatg agtctgccct gtgcatcttc atcttgaagc agatcaatga
     2701 tcgcattaag gaccgcctgc agtcctgtta taggggtgag ggaacactag acctggcctg
     2761 gctcaaggtg aaggacatcc cttgcagcag tgcgctcctt actattgatg acaacttctg
     2821 tggcctggat atgaatgctc ctctgggagt ttccgaaatg gtgcgtggca ttccagtctt
     2881 cacagaagac agggaccgca tgacttctgt catcgcctat gtctacaaga accactcact
     2941 ggccttcgtg ggcaccaaaa gtggcaagct gaagaagatc cgggttgatg ggcccaaggg
     3001 aaatgccctc caatatgaga ctgtgcaggt ggtggactca ggaccagtcc tccgggacat
     3061 ggccttttcc aaggaccacg agcaactcta catcatgtca gaaaggcagc ttaccagagt
     3121 tcctgtggag tcctgtgggc agtaccggag ctgtggcgag tgtcttggct caggtgaccc
     3181 ccattgtggc tggtgtgtgc tccacaacac gtgcacccgg aaggaacggt gtgaacgctc
     3241 cagagaaccc cgaaggtttg cctcagagat gaagcagtgt gtccggctga cggtccatcc
     3301 caacaacatc tctgtctctc agtacaacgt gctgctggtc ctagagacat acaatgtccc
     3361 ggagctgtca gcaggtgtca actgtacctt tgaagatctg tcagagatgg atgggctggt
     3421 aataggcaat cagatccagt gctactcccc tgcagccaag gaagtgcccc ggatcatcac
     3481 agaaaatggg gaccatcatg ttgtccagct gcaactcaag tcaaaggaga ctgggatgac
     3541 cttcgccagc accagctttg tcttctacaa ctgcagtgtc cacaattcat gcctgtcctg
     3601 tgtggagagc ccgtaccgtt gccactggtg taaataccgg catgtttgta ctcacgaccc
     3661 caacacatgc tccttccagg aaggccgagt gaagctacct gaggactgcc cccagctgct
     3721 gcgagtggac aagatcctcg ttccagtaga ggtgatcaag cccatcactc tgaaggctaa
     3781 gaacctccca cagcctcagt cgggacagag aggctacgag tgcatcttga acatccaggg
     3841 catcgagcag agggtacctg ctctgcgctt caacagctcc agtgtgcagt gtcagaacac
     3901 ctcctattcc tatgaaggaa tggagatcaa caacctgcca gtggagttga cagtcgtgtg
     3961 gaatggacac ttcaacattg acaacccagc tcagaataaa gtttacctct acaagtgtgg
     4021 agccatgcgc gagagctgtg ggctgtgcct caaggccgac ccggatttcg agtgtggctg
     4081 gtgtcagagc ccaggccagt gtaccctgcg ccagcactgc cctgcccatg agagccggtg
     4141 gttggaactg tcgggggcca acagcaagtg caccaaccct cgtatcacag agatcatccc
     4201 agtgacgggc cctcgagaag ggggcaccaa ggtcaccatc cgaggggaga acttgggcct
     4261 ggaattccgt gacattgcat cacacgtgaa agtggctggt gtggaatgca gccccttggt
     4321 ggatggctac atcccagctg aacagatcgt gtgtgagatg ggtgaggcca agcctagcca
     4381 gcatgcaggc tttgtggaga tctgtgtggc tgtgtgtcgg cctgagttca tggcccgctc
     4441 ttcgcagctc tattacttca tgactctgac cctcgcagat ctgaagccca accgaggacc
     4501 catgtctggg gggacacagg ttaccatcac aggtaccaac ttgaatgcag gcagcaatgt
     4561 tgtggtgatg ttcgggagcc agccctgcct cttccacagg cgctctccat cctacattat
     4621 ctgtaacact acatcctcag aggaggtgct ggacatgaag gtgactgtgc aggtggacag
     4681 ggccaggatc cgccaggacc tggtctttca gtacgtggag gaccccacca ttgtgcggat
     4741 tgaaccagaa tggagcattg tcagtgggaa cacacctatt gctgtctggg gaactcacct
     4801 ggacctcata cagaacccac agatccgtgc caagcatgga ggaaaagaac acatcaacat
     4861 ctgtgaggtc ctaaatgcta cagagatgac ctgccaggct ccagcccttg ccctgggtcc
     4921 cgaccaccag tcagatctca ctgagaggcc tgaagaattt ggtttcatcc tggacaatgt
     4981 ccagtctctg ctaattctca acaagaccaa cttcacctat tatcctaacc ccgtgtttga
     5041 ggctttcagt ccctcgggaa tcctggagct caagccgggc acccccatta tcctaaaggg
     5101 caagaacttg atcccgcctg tggccggagg caatgtgaag ctgaactaca ccgtgctggt
     5161 tggggagaaa ccatgcaccg tgacagtatc agatgtgcag ctgctctgcg agtctcccaa
     5221 cctcatcggc aggcacaaag tgatggcacg ggtgggtggc atggagtatt cccctgggat
     5281 ggtatacatc gccccagaca gcccgctcag cctgggcgcg cctggatctt ccggaatcat
     5341 cccagttgag gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc
     5401 cgccaagaag ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga
     5461 tgggatgggg gtgtctacgg tgacagctgc caggatccta aaagggcaga agaaggacaa
     5521 actggggcct gagatacccc tggccatgga ccgcttccca tatgtggctc tgtccaagac
     5581 atacaatgta gacaaacatg tgccagacag tggagccaca gccacggcct acctgtgcgg
     5641 ggtcaagggc aacttccaga ccattggctt gagtgcagcc gcccgcttta accagtgcaa
     5701 cacgacacgc ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag cagggaagtc
     5761 agtgggagtg gtaaccacca cacgagtgca gcacgcctcg ccagccggca cctacgccca
     5821 cacggtgaac cgcaactggt actcggacgc cgacgtgcct gcctcggccc gccaggaggg
     5881 gtgccaggac atcgctacgc agctcatctc caacatggac attgacgtga tcctaggtgg
     5941 gggccgaaag tacatgtttc gcatgggaac cccagaccct gagtacccag atgactacag
     6001 ccaaggtggg accaggctgg acgggaagaa tctggtgcag gaatggctgg cgaagcgcca
     6061 gggtgcccgg tatgtgtgga accgcactga gctcatgcag gcttccctgg acccgtctgt
     6121 gacccatctc atgggtctct ttgagcctgg agacatgaaa tacgagatcc accgagactc
     6181 cacactggac ccctccctga tggagatgac agaggctgcc ctgcgcctgc tgagcaggaa
     6241 cccccgcggc ttcttcctct tcgtggaggg tggtcgcatc gaccatggtc atcatgaaag
     6301 cagggcttac cgggcactga ctgagacgat catgttcgac gacgccattg agagggcggg
     6361 ccagctcacc agcgaggagg acacgctgag cctcgtcact gccgaccact cccacgtctt
     6421 ctccttcgga ggctaccccc tgcgagggag ctccatcttc gggctggccc ctggcaaggc
     6481 ccgggacagg aaggcctaca cggtcctcct atacggaaac ggtccaggct atgtgctcaa
     6541 ggacggcgcc cggccggatg ttaccgagag cgagagcggg agccccgagt atcggcagca
     6601 gtcagcagtg cccctggacg aagagaccca cgcaggcgag gacgtggcgg tgttcgcgcg
     6661 cggcccgcag gcgcacctgg ttcacggcgt gcaggagcag accttcatag cgcacgtcat
     6721 ggccttcgcc gcctgcctgg agccctacac cgcctgcgac ctggcgcccc ccgccggcac
     6781 caccgacgcc gcgcacccgg gtcatcatca ccatcaccat taatctagag atcttattaa
     6841 agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6961 tatcttatca tgtctggtcg actctagcta gactcttccg cttcctcgct cactgactcg
     7021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7801 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7861 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7921 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7981 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8041 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8101 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8161 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8221 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8281 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8341 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8401 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8461 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8521 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8581 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8641 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8701 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8761 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8821 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8881 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8941 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     9001 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagga atgtgtgtca
     9061 gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc
     9121 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9181 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     9241 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9301 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     9361 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcgtcccgg
     9421 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9481 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     9541 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     9601 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     9661 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     9721 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     9781 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     9841 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     9901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     9961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
    10021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
    10081 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    10141 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    10201 tgtaaaacga cggccagtga att
//
