LOCUS       sequence-135630-       12452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sdk1 protein (endogenous 
            signal peptide replaced with CD33 signal peptide), C-terminally 
            fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sdk1-AP-His
FEATURES             Location/Qualifiers
     source          1..12452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             9032..9049
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    9075..9196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9112..9131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9166..9185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9234..9251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9405..9993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9485..9504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10164..10955,10956..11024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10787..10806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11025..11129)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11197..11215
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11253..11275)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11277..11297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11282..11612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11463..11598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11525..11544
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     11749..11768
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(11835..12290)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11972..11993)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12184..12203
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12416..12438
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12430..12447
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12431..12447
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattcgccac catgcccctg ctgctgctgc tgcccctgct
     1621 gtgggccggc gcggccgctg gacctggaag gcctaggagg agcaggaccg gtcaccactg
     1681 cgaccccgag tgcccagggc tgcgcgcagc gcccaggacc cccgggccgg gcgcgggccg
     1741 gcgggcggca aagttgaggc cgggccgcgg gtggtgggcg ctgctgttgc tccagctgca
     1801 cttgctgcgg gcgctggcgc aagatgacgt tgcaccgtat ttcaaaacgg agccgggcct
     1861 gccacagatc cacctggaag ggaaccgcct ggtgctcacc tgcctggctg aagggagctg
     1921 gcctctggaa ttcaagtgga tacggaacga cagtgagctt accacctaca gcagcgaata
     1981 taagtatatc atcccctctt tgcagaagct ggacgccggg ttttaccgct gcgtggtgcg
     2041 gaacaggatg ggagctctct tgcagagaaa atcagaaatt caagtagcat atatggggaa
     2101 cttcatggat acagaccaga ggaaaacagt ttctcaaggg catgcggcac ttctcaactt
     2161 actgcctatc gtcagctgcc cccaacctca agtgacgtgg tttagagaag gacacaagat
     2221 tattcccagc agcagaatag ccatcacatt ggagaatcag ctggtgatcc ttgctacaac
     2281 agcgagtgat gctggggctt actacgtgca ggctgtgaat gagaagaacg gagagaacaa
     2341 gacaagcccg ttcatccacc tgagcgtagc acgagatact ggtacccacg aggccatggc
     2401 cccaatcatc gtggttgccc caggcaacag aagtgtggta gcagggtcca gtgagaccac
     2461 cttggagtgc atagccaatg ccaggcccgt ggaggagctg agtgtgcact ggaagagaaa
     2521 cggagttcga ctcacaagtg gactacacag ctacgggagg cgccttacca tcaccaatcc
     2581 gacatcagca gacaccggga tgtacgtgtg cgaggcgaca ctccggggga gcacatttga
     2641 gccagccagg gccagagcct tcctttccat catagagcca ccatatttca ctgctgagcc
     2701 agagagtcgg attttagggg aagtggaaga aactatggac atcccatgtc gagccatggg
     2761 ggttcctctt cccactctcc agtggtacaa agatgctgtt cccctcagca aactccagaa
     2821 ccctaggtat aaagttctcc ccagcggagg cctccacatt cagaagttga gtccagaaga
     2881 ctctggaatc ttccagtgct ttgccagtaa cgaaggagga gaggtccaga ctcacaccta
     2941 cctggatgtg accaatattg ctcctgcctt cacccagcgt ccagtggaca ccacggttac
     3001 tgacgggatg acggctgtcc tgaggtgtga ggtgtccgga gctcccaaac ccgccatcac
     3061 atggaagaga gggaaccaca ttctggccag tggctctgtc cggattccta ggttcatgct
     3121 gctggaatct ggggggctga gaatagcccc cgtcttcatc caggatgctg gcaactacac
     3181 ctgctatgca gccaacacgg aggcttctgt gaacgcctcc gccatgctca ccgtgtggaa
     3241 tcgaacgtcc atcgttcacc ctccagaaga ccgcgtggtg attaagggga cgacggccac
     3301 cctgtgctgc ggagctaccc acgaccctcg gacctctctc cgctacgttt ggaaaaagga
     3361 caacgtggtc atcacagcgt ctagcagctc cagaattgtg gtggaaaagg atgggtcgct
     3421 tgtcattagc cagacgtggt caggggacat tggtgactac acctgtgaaa tcatatccga
     3481 gggagggagt gactcccgga cggcacggct ggaagtgatt gagctgcctc acccacctca
     3541 aaacctgctg gccagcttga gtccagcacg cagccactcg gtgacactat cttgggttcg
     3601 gccctttgat ggaaacagcc cggtccttta ctacattgtg caagtatctg aaaacaactc
     3661 tccatggaaa gtgcatttgt caaatgttgg cccggagatg acaggcgtca ctgtgagtgg
     3721 cttgactcca gcccggacct atcagttcag ggtgtgcgct gtgaatcaag tgggcaaggg
     3781 ccagtacagc acagagacaa gcagattgat gttacccgaa gaaccaccga gtgctccccc
     3841 aaaaaacatt gtggccagtg ggagaaccaa ccagtccatc atggtacagt ggcagccacc
     3901 acccgaaaca gagcacaatg gggtgcttcg gggctacatc ctcaggtacc gcctggctgg
     3961 cctgcctggg gaacaccagc agaggaacat cagcagccca gaggtgaact actgcctggt
     4021 caccgacctc atcatctgga cccagtatga gatccaggtg gctgcctaca acggggcagg
     4081 cctgggtgtc ttcagcaggg cagtgaccga gtacacctta caaggagtgc ccactgcacc
     4141 tccccagaac gtgcaggcag aggctgtgaa ttccaccact gtgcacttcc tgtggaaccc
     4201 gccaccccag cagttcatca acggcatcaa ccagggatac aagctcctag catggccggc
     4261 agatgctcct gagactgtca ctgtagtcac tattgctcct gacttccatg gaatccatca
     4321 tgggtacata acaaacttga agaagttcac tgcctacttc acatccgtcc tgtgttttac
     4381 caccccgggg gatgggcctc caagttcacc tcagctcgtg tggacccatg aggacaagcc
     4441 gggagctgtg gggcacctga gcttcacgga gattttggac acctctctca aggtcagctg
     4501 gcaggagccc ctggagagaa acggcatcat tatgggctat cagatttcct gggaggtgta
     4561 tgggaggaat gactctcgac tcacgcacac cctcaacagc acgatgcacg agtataagat
     4621 ccaaggactg tcatcactca ccacctatac catcgatgtg gctgccttga ccgccgcagg
     4681 cgtgggcgtg accacatcat ccaccatctc ttcaggtgta cccccagatc ttcctggcgc
     4741 tccatccaat ctggtcattt ccaacatcag ccctcgctca gccacactcc agttccgacc
     4801 tggctatgat gggaagacgg ccatctgcag gtggattgtg gaggggcagg ttggtgccat
     4861 cggggacgag gaggagtggg ttaccctcta tgaggaggag aatgagcccg atgcccagat
     4921 gttggagata ccaaacctca caccctacac acactacagg tttcgaatga ggcaagtgaa
     4981 catcgtgggt cccagcccct tcagccagtc atccagggtc atccagaccc tacaggcccc
     5041 acctgatgtg gctccaacca gcctcactgt ccgaacagcc agtgagacca gcctgaggct
     5101 acgctgggtg cctctgcctg attcccagta caatgggaac cctgagtctg tgggctacag
     5161 ggtgaagtat tggcgttctg accaaccatc gtcagctctg gcccaagttg tcagtgaccg
     5221 tctggagagg gagctgacca tcgaggaact ggaggagtgg acagagtacg agttgcggat
     5281 gcaggccttc aacgccattg gggctgggcc ctggagtgag ctggtgcggg gccgcacgcg
     5341 ggagtcagtt ccttcagcag ctcctgaaaa tgtgtctgca gaggctgtca gctccaccca
     5401 gatcctactg acttgggctt cagttcctga gcaggaccag aatgggctca tcctaggcta
     5461 caaggttcta tactgtgcca aagacctgga cccagagccc agaagccacg tggtacgagg
     5521 gaaccacacg cagtctgcgc tgctggctgg cctgcgaaag ttcgtggtct atgagctcca
     5581 agtgctggcg ttcacacgga tcgggaatgg ggtccccagc tcacctctca tcctggagcg
     5641 caccaaagat gacaccccag gtccccccgt gcggctggtg ttccccgaag taagacttac
     5701 tgcagtgcgg attgtgtggc agcccccaga ggagcccaat ggtgtgattc tggggtacca
     5761 gatcgcctac cgcctggcca gcggtagtcc tcacaccttc accactgttg aggtgggtgc
     5821 cacggtgcgg caattcacag ccacagagct ggccccggag tctgcctaca tcttcagact
     5881 gtctgctaag acgagacagg gttggggtga gcccctggag gccacagtta tcactactga
     5941 gaagagagag aggccagcgc cccccagaga gctcctggta ccccaggctg aagtgacagc
     6001 tcgtagcctc aggctccagt gggtccctgg cagtgatggg gcctctccga tccggtactt
     6061 cacggtgcag gtgcgagagc tgccaggtgg agagtggcag acctactcct catctatcag
     6121 tcatgaggcc acagcctgtg ctgttgagag gctgaggcct ttcacctcct acaagctgcg
     6181 cctgaaggcc accaatgaca ttggggacag tgacttcagt gcagaaacag aggctgtgac
     6241 cactttgcaa gatgttccag gagagccacc agggtctgtc tcagccacac cgcacaccac
     6301 gtcctccgtt ctgatccagt ggcagcctcc ccgggatgag agcctgaacg gtcttctgca
     6361 aggctacagg atctactacc gtgagctgga gtctgagaca ggcatgagcc ctgagcccaa
     6421 gacactcaag agcccctctg ccttacgtgc tgaactcacc gctcaaagca gcttcaagac
     6481 cgtgaacagc agctcctcgt taaccaccta tgaattaaca catctgaaga agtaccggcg
     6541 ctatgaagtc atcatgactg cttataacat cattggtgag agcccagcca gtgtaccagt
     6601 ggaagtcttc gttggtgagg ctgccccagc gatggcccca cagaatgtcc aggtgacccc
     6661 actcacggcc agccagctgg aggtcacatg ggacccaccg cccccagaga gccagaatgg
     6721 gaacatccag ggttataagg tttactactg ggaggcagac agtcgcaatg agacagagaa
     6781 aatgaaggtc ctcttcctcc ccgagcctgt ggtgaagatc aaggatctca ccagccacac
     6841 aaagtacctg atcagcatct cagccttcaa tgctgctggt gatgggccca agagtgaccc
     6901 gtgccaagga cgcacacatc aggcggctcc agggcccccc agcttcttgg cattctcaga
     6961 aataacttct accacactca acgtatcctg gggagagcca tcagctgcca acggcatcct
     7021 acagggctat cgagtggtgt atgagccctt agcaccagtg caaggcgtga gcaaggtggt
     7081 gaccgtggac gtgaaaggca actggcaacg ttggctgaag gtgcgggacc tcaccaaggg
     7141 agtgacctac ttcttccgtg tccaggcgcg gaccatcgcc tacgggccgg agctccaagc
     7201 caatgtcact gcagggccag ccgaggggtc cccaggctca ccaagaaatg tccttgtcac
     7261 caaatctgcc tctgagctga cccttcagtg gacagaggga aatgctggta ccacacccac
     7321 tacaggctac gtcatagaag ccaggccatc agatgaaggc ttatgggaca tgtttgcaaa
     7381 ggacattccc aggagtgcta catcatacac tgtggacctg gataagttgc ggcaaggggt
     7441 gacctacgag ttccgggtgg tggctgtgaa caaggcaggc tttggggagc ccagccgccc
     7501 ttccattgca gtgtcagcac aagctgaagc cccgttctac gaggagacta gtggatcttc
     7561 cggaatcatc ctagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc
     7621 cctgggtgcc gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt
     7681 cctgggcgat gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa
     7741 gaaggacaaa ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct
     7801 gtccaagaca tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta
     7861 cctgtgcggg gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa
     7921 ccagtgcaac acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc
     7981 agggaagtca gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac
     8041 ctacgcccac acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg
     8101 ccaggagggg tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat
     8161 cctaggtggg ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga
     8221 tgactacagc caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc
     8281 gaagcgccag ggtgcccggt acgtgtggaa ccgcactgag ctcatgcagg cttccctgga
     8341 cccgtctgtg acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca
     8401 ccgagactcc acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct
     8461 gagcaggaac ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca
     8521 tcatgaaagc agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga
     8581 gagggcgggc cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc
     8641 ccacgtcttc tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc
     8701 tggcaaggcc cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta
     8761 tgtgctcaag gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta
     8821 tcggcagcag tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt
     8881 gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc
     8941 gcacgtcatg gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc
     9001 cgccggcacc accgacgccg cgcacccggg tcatcatcac catcaccatt aatctagaga
     9061 tcttattaaa gcagaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9181 tcatcaatgt atcttatcat gtctggtcga ctctagctag actcttccgc ttcctcgctc
     9241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10441 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11101 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11161 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    11221 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggaggaa
    11281 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa
    11341 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    11401 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    11461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    11521 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
    11581 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
    11641 tcgtcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    11701 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    11761 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    11821 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
    11881 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    11941 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    12001 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
    12061 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    12121 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    12181 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    12241 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
    12301 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    12361 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    12421 tcacgacgtt gtaaaacgac ggccagtgaa tt
//
