LOCUS       sequence-135636-       12305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sdk2 protein, C-terminally
            fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 12305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sdk2-AP-His
FEATURES             Location/Qualifiers
     source          1..12305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             8885..8902
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8928..9049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8965..8984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9019..9038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9087..9104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9258..9846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9338..9357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10017..10808,10809..10877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10640..10659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10878..10982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11050..11068
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11106..11128)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11130..11150)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11135..11465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11316..11451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11378..11397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     11602..11621
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(11688..12143)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11825..11846)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12037..12056
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12269..12291
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12283..12300
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12284..12300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtggaggct
     1621 gccgctctgg actctactgg ccctgcatcg gatccactcg gccggggccc aagatgatgt
     1681 accgccatac ttcaagacgg agccagtgcg cacacaagtg catctggaag ggaaccgcct
     1741 ggtgctcacc tgcatggctg aaggcagctg gccgctggag ttcaagtggc tacacaacaa
     1801 tcgggagctg acccggttct cgctggagta caggtacatg attaccagcc tggaccgcac
     1861 ccacgctggc ttctaccgct gcatcgtgcg taaccgaatg ggggccctgc tacagcggca
     1921 gactgaagtc caggtggcct acatggggag ctttgaggaa ggcgagaagc gccaaagtgt
     1981 gaaccatgga gaagctgctg tcatcagagc acctcgcatt tccagcttcc cccggccaca
     2041 ggtgacctgg ttccgtgacg gacgcaagat cccacccagc agccgcatag ccatcaccct
     2101 ggagaacacc ctggtcatcc tgtcgacggt ggctcccgat gctggccgct actacgtgca
     2161 ggcggttaat gacaagaacg gagacaacaa gaccagccag cccatcactc ttgccgtgga
     2221 gaatgttggg ggacccgctg accccattgc accaaccatc atcatcccac ccaagaatac
     2281 cagtgtcgtg gctgggacct cggaggtgac catggagtgt gtggccaacg ccaggcctct
     2341 catcaagctg cacattgtgt ggaagaagga cggggctcca ctctccagtg gcatcagcga
     2401 ctacaaccgc aggctcacca tcgccaaccc cacggtcagc gacgccggct actacgagtg
     2461 cgaagccatg ctgcgcagca gcagcgtggc ccctgtcacc cggggcgcct acctctcggt
     2521 gctggagcca cctcagtttg tcagagagcc tgagagacat atcacggccg agatggaaaa
     2581 ggtggtggac atcccctgtc gagccaaagg ggtaccgcct ccctccatca cctggtacaa
     2641 ggacgctgcg ttggtggagg tggggaagct aacccgcttc aagcagcgca gcgatggcgg
     2701 cctgcagatc agcggcctgc tgcccgacga caccggcatg ctccagtgct ttgcccacaa
     2761 tgcagctggc gaggcccaga cctccaccta cctggctgtc accagcattg cccctaatat
     2821 caccagaggc ccccttgaca gcacggtgat tgacggcatg tcggtggtac tagcatgtga
     2881 gacctcaggg gcaccacggc ctgctatcac ttggcaaaaa ggggagcgca tcttggccag
     2941 tggctctgtc cagctgcctc gattcacact gctggaatct ggcagcctgc tcattagccc
     3001 aacccacatc tcggacgccg gcacctacac ttgtttggcc accaactctc ggggagtaga
     3061 cgaggcgtca gcagacctgg tggtgtgggc ccggacacgc atcactaagc caccccagga
     3121 ccagagtgtc atcaagggca cccaggcctc catggtgtgt ggagtgaccc acgatcccag
     3181 agtgactgtc aggtacgtgt gggagaagga tggggccacc ctggccgtgg agactaatcc
     3241 tcggatccgc ctggacagaa atggctccct acacatctcg cagacgtggt caggggacat
     3301 tggcacgtat acctgccggg tgctctcggc tggtggcaat gactctcgca acgcccacct
     3361 gcgagtcagg cagctccccc atgcccctga gcaccctgtg gcaaccctca gcaccgtgga
     3421 gagacgcgcc atcaacctga cctgggccaa acccttcgac ggcaacagcc ctctgatgcg
     3481 ctacgtcttg gagatgtcag aaaacaatgc tccctggacc atactcctgg ccagcgtgga
     3541 cccagaagct acctcagtga tggtcaaggg actggttcct gctcgatcct accagttccg
     3601 cctgtgcgct gtcaatgatg tgggcaaagg acagttcagt aaggacacag aaagggtgtc
     3661 ccttcctgag gagcccccca cggcccctcc gcagaacgtc atcgccagcg gccggaccaa
     3721 ccaatccatc atgatccaat ggcaaccacc tcccgagagc caccagaatg ggatcctcaa
     3781 gggctacatc atcagatact gcctggcagg gctacctgtt gggtaccaat ttaagaacat
     3841 cacagatgct gacgtcaaca acctgttgct ggaggacctc atcatatgga ccaattacga
     3901 gatcgaggtg gctgcctaca acagcgccgg gctgggtgtc tacagcagca aagtcaccga
     3961 gtggacgctg cagggagttc ccacggtgcc cccgggcaat gtgcatgcag aagccacaaa
     4021 ttctacaacc atccgattca cctggaatgc cccaagcccg cagttcatca acggcatcaa
     4081 ccagggctac aagctgattg cctgggagcc agcccaagaa gaggaggtta ccatggtgac
     4141 cgccagaccc aactttcaag acagcatcca tgtgggcttc gtgtccggcc tgaaaaaatt
     4201 cacagagtac tttacctcgg tcctctgctt caccacaccg ggagacgggc ctcggagcag
     4261 cccccagctg gtgcgcacac acgaggacgt acctggaccc gtgggacacc tgagcttcaa
     4321 tgacatcctg gacacctcgc tgaaggtcag ctggcaggag ccaggcgaga agaacggcat
     4381 cctgacaggg taccggatct cctgggaaga gtacaacaga accaacaccc gtgtgaccca
     4441 ctacctgccc aacgtaaccc tggagtaccg agtcacgggc ctcacagcac tcactaccta
     4501 tactatagag gtggccgcca tgacctccaa gggccagggc caggtgtcgg cctccactat
     4561 ttcatctggt gtgcctccag agcttccagg ggcacccacc aacctgggca tttccaacat
     4621 cggcccacga tccgtgacct tgcagttcag gcctggctat gacgggaaga cgtccatctc
     4681 ccgctgggtg gtggaggctc aggtgggcgt gatcggggag ggcgaggagt ggttgctcat
     4741 ctaccaactc ggcaatgagc ctgacgcccg ttccatggag gtgccggacc tcaacccctt
     4801 cacctactac agcttccgca tgcgccaagt gaacatcgtt ggcaccagcc cacccagtca
     4861 accttctaga aagatccaga ccctccaggc gcccccagac atagctccag ccaacgtgac
     4921 tctgcgcaca gccagtgaga ccagcttgtg gctccgatgg atgcccctcc cagaaatgga
     4981 atacaacggc aaccctgagt cggtgggcta caagatcaag tacggccgct ctgacggcca
     5041 cggcaagacg ctgagccaca cggtacaaga ccgagtggag cgagaataca ctattgagga
     5101 cctggaggag tggacagagt accgcgtcca agttcaggcc ttcaacgcca tcgggagcgg
     5161 gccgtggagc caggcagtgg tgggcaggac ccgggagtca gtcccatcct ctggccccac
     5221 caacgtgtcg gctctggcca ccacctctag cagcatgctg gtccgctgga gcgagatccc
     5281 ggaggcagat cgaaatggcc tcgtgctggg ctacaaggtg aggtacaagg agaaggactc
     5341 ggactcccag ccccggttct ggctggtaga aggcaactca tcccgcagcg cccagctcac
     5401 aggcctgggc aagtatgtgc tctacgaggt ccaggttctg gccttcacac gcatcggcga
     5461 cggcagcccg agccaccctc ccatcctgga gcggacacta gatgatgtcc caggcccccc
     5521 catgggcatc ctgtttccag aggtgagaac cacatctgtg cggctcatct ggcagccccc
     5581 agcagctccc aacggcatca ttctggccta ccagatcaca caccgtctca acgccactac
     5641 agccaacacc gccacagtgg aggtcctggc ccccagcgcc cgccagtaca tggccacagg
     5701 cctcaagccg gagtctgtct acctattccg tatcacagcc caaacccgca aaggctgggg
     5761 agaggctgct gaggccttgg tggtgactac agagaagaga gaccggccgc agccccctag
     5821 caagcccgtg gtacagcagg aagacgtgaa agcacgcagt gtgctgctgt cctgggaacc
     5881 aggcagcgat gggctctccc cagtacgcta ctacaccatc cagacgcgcg agctgcccag
     5941 cggcaggtgg gcgctgcact cggcctccgt gagccacaat gcctccgcct tcactgtgga
     6001 cagactcaaa ccctttacgt cctacaagtt ccgagtgaag gcgaccaacg acatcggcga
     6061 cagcgagttc agcgaggaat cggagtcgct gaccaccctg caggccgccc cagacgaggc
     6121 acctaccatc ctgtcagtga ctccacacac caccacctct gtgctaatcc gatggcagcc
     6181 tccatctgag gacaagatca acggcatcct cttgggcttc cggatccggt accgggagct
     6241 gctctacgat gggctgaggg gcttcactct tagaggcatc aacaaccctg gggccaagtg
     6301 ggctgagctc acctccttgt actccatgcg gaacctgacc cggcccagcc tcacgcagta
     6361 cgagctggac aatctgagca agcacaggcg ttacgagatc cgaatgagca tatacaacgc
     6421 cgtgggtgag ggacccttga gcccgccgca agaggtcttt gttggggagg cagtgcccac
     6481 ggcagcaccg cagaacgtgg ccatccacag tgccacagcc acacagctgg atgtgacctg
     6541 ggagccaccc cctttggaca accagaatgg agacatccaa ggatacaaga tttatttctg
     6601 ggaagtccaa cggaggaacc tcacagagag ggtgaagact ctcttcttgg ctgagaacag
     6661 cgtgaaactc aagaacctga cgggctacac agcctacatg gtcagcgtgg cagccttcaa
     6721 tgctgcgggg gatgggccga ggagcacccc gacccggggc cagactcagc aagcagcccc
     6781 cagtgccccg ggctcggtca agttcagcga gctgaccact acctcagtga atgtgtcctg
     6841 ggatgcccca cagttcccca acggtcctct ggagggctac aggctggtgt acgaaccctg
     6901 caccccagtg gatggggtca gcaagattgt gacagtggac gtgaagggga acagcccact
     6961 gtggctgaaa gtgaaggatc tggccgaggg catgacgtat cgtttccgaa tcaaagccaa
     7021 gaccttcacc tatgggcctg agattgaagc caacatcact acgggccctg gagaaggtgc
     7081 cccaggacca ccgggagtac cgatcatcgt tcgctacagc tcagccattg ccatccactg
     7141 gtccagcgga gatcctggca aagggcccat cacgcgctac gtaatagagg ccaggccttc
     7201 agatgagggg ctctgggaca ttctcatcaa ggatatcccc aaggaggtga cttcctacac
     7261 cttcagcatg gacatcctga agccaggcgt aagctatgac ttccgggtca tcgcagtcaa
     7321 cgactacggc tttggcactc ccagcagccc ctcccagtct gtgccagccc agaaagccag
     7381 ccccttttat gaggaaggcg cgcctggatc ttccggaatc atcccagttg aggaggagaa
     7441 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     7501 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     7561 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     7621 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     7681 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     7741 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     7801 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     7861 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     7921 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     7981 gcagctcatc tccaacatgg acattgacgt gatcctaggt gggggccgaa agtacatgtt
     8041 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     8101 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
     8161 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     8221 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     8281 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     8341 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     8401 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     8461 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     8521 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     8581 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     8641 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     8701 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     8761 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     8821 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     8881 gggtcatcat caccatcacc attaatctag agatcttatt aaagcagaac ttgtttattg
     8941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt
     9061 cgactctagc tagactcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10981 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    11041 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    11101 gaaaacctct gacacatgca gctcccggag gaatgtgtgt cagttagggt gtggaaagtc
    11161 cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    11221 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    11281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
    11341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    11401 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    11461 gcaaaaagct cccgggagct tgtatatcca ttttcgtccc ggagacggtc acagcttgtc
    11521 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    11581 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    11641 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
    11701 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
    11761 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
    11821 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
    11881 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
    11941 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
    12001 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
    12061 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
    12121 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
    12181 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    12241 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    12301 gaatt
//
