LOCUS       sequence-135640-       10934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the entire extracellular region of the PlexinB1 protein 
            (ie, long), C-terminally fused to alkaline phosphatase + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb1(L)-AP-His
FEATURES             Location/Qualifiers
     source          1..10934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      4038..4047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7514..7531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7557..7678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7594..7613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7648..7667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7716..7733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7887..8475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7967..7986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8646..9437,9438..9506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9269..9288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9507..9611)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9679..9697
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9735..9757)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9759..9779)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9764..10094
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9945..10080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10007..10026
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     10231..10250
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(10317..10772)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10454..10475)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10666..10685
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10898..10920
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10912..10929
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10913..10929
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtctgtcct
     1621 cggcccagtt cttctccagg tgttctgggc cgggtgtgtc gtcaccctgc ggtcccctct
     1681 gccagctgct ttcactgcca atggcacaca tctacaacac ttggcaaggg accccaccac
     1741 aggtaccctc tatgtagggg ccaccaactt cctgttccag ttgagccctg ggctgcagct
     1801 ggaagccgtg gtgtccacgg gccctgtgaa tgacagccgg gattgcctgc cacctgtgat
     1861 acctgatgaa tgtccccaag cccagcctac taacaaccct aaccagctgc tcctggtgag
     1921 cccagaggct ctggtggtgt gtgggagcat acaccagggc atctgtgagc tacggagcct
     1981 gggacagatc aggcagctgc tgctacggcc agagcgacct ggggacaccc agtatgtggc
     2041 tgcaaatgac cctgcagtca gtacagtggg gctggtggcc cagggattgg taggggagcc
     2101 cctcctgttt gtggggcggg gatacaccag caggggtgta ggtggtggga ttcctcccat
     2161 tacaacccga gccctgcgac caccggaccc ccaagctgcc ttctcttatg aagaaacagc
     2221 caagttagca gtgggccgcc tgtccgagta cagccaccac ttcgtgagtg cctttgtacg
     2281 cggggccagt gcatacttcc tgttcttgcg gcgagacctg aaggcccctt ctagagcttt
     2341 ccgtgcctat gtgtctcgag tgtgccttca ggaccagcac tactactctt atgtggaatt
     2401 gcccctggcc tgccagggtg gtcgttacgg tcttatccag gctgcagctg tagccacgtc
     2461 caaggaggtg gcccgtgggg acgtactctt tgcagctttc tcctcagtgg ctcctcccac
     2521 tgtggattgg cccctgtcag catctactgg ggcatctgga acctctgtgc tctgtgcctt
     2581 ccccctggat gaggtagacc agcttgctaa ttacactcga gatgcctgtt atactcggga
     2641 aggccgtgct gagaacggga ccaaggttgc tgacattgca tacgatgtcc tttccgactg
     2701 tgcgcagcta ccagtggaca ccccggatgc ttttccatgt ggctctgacc acacacccag
     2761 tcccatggtc agctgtgtcc ctttggaagc cacgccaatt ctggagctac caggggttca
     2821 gctaacagct gtggctgtca ccatggagga tggacacact attgctttcc tgggtgacag
     2881 tcaaggacag ttgcataggg tctacttagg ccctggaaga agtgctgccc catattctaa
     2941 acagagcatc cagccggggt ctcctgtgaa cagagatctt acctttgatg gtacctttga
     3001 gcatctctat gtagcaactc agactactct tgtgaaggtt cctgtggctc cttgtgctca
     3061 gcatctggac tgtgactctt gccttgctca cagggaccct tattgcggat ggtgtgtgct
     3121 cctgggcagg tgtagtcgcc ggtcggagtg ctcaagggac cagggcccag agcagtggct
     3181 gtggagcttc cagccggaac tgggctgtct tcgagtggtg gccgtgagcc ctgccaatat
     3241 cagtcgggaa gagaggaggg aggttttctt gtcagtgcca ggcctgccat ctctctggcc
     3301 aggggagtca tatttctgct actttggaga ccaacagagt cctgctctac tgaccagttc
     3361 tggtgtgatg tgtccctccc cagaccccag tgaggctcca gtgctgcaga gaggagccga
     3421 ccatatctct gtgaacgtgg agctcaggtt tggtgccgtg gtgatcgcca gcacctccct
     3481 ctccttctat gactgcgtgg cagttactgc gtcttcccca tctgcaccgt gccgggcctg
     3541 tgtgagcagc cgctggggct gtaactggtg tgtgtggcag cagctgtgca cacacaaggc
     3601 ctcgtgtgac gctgggccta tggtggcaag ccaacagagc ccactccttc ccctaatccc
     3661 tcctgcaagg gatgaactca cccctttccc acccacagtc ccccaaacca cggtcactcc
     3721 tacccccaac agcttcccaa tagagcctag ggctccctcc acagcctcag atgtcctacc
     3781 tggggccaag ccttcccggc tcagcctctg gggcccatgg gcaggtcctg gccccatact
     3841 ttcccctact tccacagagt cacctcttca tgagaagccc cttcctcctg acccccctac
     3901 catacctgga accactgtcc ctgcccccac tggcttggga ccatcgacca cacctgagga
     3961 cctcttggcc tcctacccat tcccctcaga tgcagctgca gtgtcccctg cagagcctgg
     4021 ccctgaggct ctgccttcca tggtggctct ggaccagccc cctggcactg ttccagacac
     4081 tactttccca ggggcccctg gctccatgaa gcccgttctg gattggctca ccaaaggagg
     4141 cggcgagctg cccgaggcgg atgagtggat ggggggtgac acgcccgcct tctccacttc
     4201 cacactcctc tcaggtgatg gagactcagc agagcacgag ggccctcctg cccccctcat
     4261 cctcctgtcc agcctcgact accagtacga cacccccggg ctctgggagc tgggagaggt
     4321 gaatcagagg gtgagctcct gcccctgtgt ggagaccgtc cagggctcct tgctgatacc
     4381 ggtccatgtg gaacgcgaag tccagcttcg aggcaggaac ctgtggcttt tccaggatgg
     4441 cccgaggagc agcgagtgtg tgctggagct agggagtcgg gaggtggctg tggaggctca
     4501 ggtggagtgt gcgccgcctc cagatgtctg gtgccacatc aagtgccagc agcatcagtt
     4561 cagctatgaa gctttgaagc cagaactgca ggtggggctg ttcctgcgtt gggcaggcgg
     4621 tctgcgcgtg gacagtgccg atgggctgca tgtggtgttg tatgactgct ctgtgggaca
     4681 tggggactgc agccgctgcc aaactgccat gcctcagtac gactgtgtgt ggtgtgaggg
     4741 ggagcgtccg cgttgtgtgg cccgggaagc ctgtaatgaa gccgagactg tggccactca
     4801 gtgccccgca cctctcattc actcggtgga tccactgact ggacctatag atggaggcac
     4861 ccgtgtcact atcaggggct ccaacctggg ccaacatgtg caggatgtcc tggacatggt
     4921 cagagtggcc ggagttccct gcgctgtgga tgctggggag tatgatgtct ctagtagtct
     4981 tgtgtgcatc actggagcca gcggggagga ggtgactggc actgtggcag tggaggtgcc
     5041 tggaagagga cacggtgtct cagagttcag ctttgcctat caggatccaa aagtacactc
     5101 catcttccca gcccgtggcc ctagagctgg aggtacccgc cttaccctgc atggttctaa
     5161 gctcctgact ggacggctag aggacatccg tgtggtggtt ggagaccagc cttgccacct
     5221 gctcctggag cagcagtctg agcagctaca ctgtgagacc ggcccatacc ctgtgcctgc
     5281 tgaacttcca gtgactgtct tgtttggggc cactgagcgg aggcttcagc acggccaatt
     5341 caagtataca tcagacccca atgtcacctc agtgggcccc tccaagagct tcttcagcgg
     5401 aggacgtgag atatgggtcc gcggccagga tcttgatgtg gtacagaggc caagaatccg
     5461 agtgaccgtg gtcccaagac agcatggcca ggggcttgca cagaagcaac acgtggtccc
     5521 tgagaaattt gaggagccgt gtctcgtgaa ctcctcccac ctcctcatgt gccgcactcc
     5581 cgctctccct ggcccaccct gggactctgg ggtccaggtg gagtttatcc tcgacaacat
     5641 ggtctttgac tttgctgcac tgagccccac acccttctcc tatgaggctg atcccaccct
     5701 gcgttccctg aaccccgagg atcccagcac gccgttccgg cacaagccag ggagtgtgtt
     5761 ctctgtggag ggggagaatc tggacctcgc catgtctaaa gaagaggtgg tggccatgat
     5821 aggggacggg ccctgcgtgg taaagacact gacccggaac cacctgtact gtgagccccc
     5881 tgtggagcag cccctgccac atccccatgc cctccgagag gctccagatg ctttgcctga
     5941 gttcacggta cagatgggca acctgcgctt ctccttgggt catgtgcagt acgatggcga
     6001 gagccccgtg gcttttcctg tggcaggcgc gcctggatct tccggaatca tcccagttga
     6061 ggaggagaac ccggacttct ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa
     6121 gctgcagcct gcacagacag ccgccaagaa cctcatcatc ttcctgggcg atgggatggg
     6181 ggtgtctacg gtgacagctg ccaggatcct aaaagggcag aagaaggaca aactggggcc
     6241 tgagataccc ctggccatgg accgcttccc atatgtggct ctgtccaaga catacaatgt
     6301 agacaaacat gtgccagaca gtggagccac agccacggcc tacctgtgcg gggtcaaggg
     6361 caacttccag accattggct tgagtgcagc cgcccgcttt aaccagtgca acacgacacg
     6421 cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt
     6481 ggtaaccacc acacgagtgc agcacgcctc gccagccggc acctacgccc acacggtgaa
     6541 ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga
     6601 catcgctacg cagctcatct ccaacatgga cattgacgtg atcctaggtg ggggccgaaa
     6661 gtacatgttt cgcatgggaa ccccagaccc tgagtaccca gatgactaca gccaaggtgg
     6721 gaccaggctg gacgggaaga atctggtgca ggaatggctg gcgaagcgcc agggtgcccg
     6781 gtatgtgtgg aaccgcactg agctcatgca ggcttccctg gacccgtctg tgacccatct
     6841 catgggtctc tttgagcctg gagacatgaa atacgagatc caccgagact ccacactgga
     6901 cccctccctg atggagatga cagaggctgc cctgcgcctg ctgagcagga acccccgcgg
     6961 cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt catcatgaaa gcagggctta
     7021 ccgggcactg actgagacga tcatgttcga cgacgccatt gagagggcgg gccagctcac
     7081 cagcgaggag gacacgctga gcctcgtcac tgccgaccac tcccacgtct tctccttcgg
     7141 aggctacccc ctgcgaggga gctccatctt cgggctggcc cctggcaagg cccgggacag
     7201 gaaggcctac acggtcctcc tatacggaaa cggtccaggc tatgtgctca aggacggcgc
     7261 ccggccggat gttaccgaga gcgagagcgg gagccccgag tatcggcagc agtcagcagt
     7321 gcccctggac gaagagaccc acgcaggcga ggacgtggcg gtgttcgcgc gcggcccgca
     7381 ggcgcacctg gttcacggcg tgcaggagca gaccttcata gcgcacgtca tggccttcgc
     7441 cgcctgcctg gagccctaca ccgcctgcga cctggcgccc cccgccggca ccaccgacgc
     7501 cgcgcacccg ggtcatcatc accatcacca ttaatctaga gatcttatta aagcagaact
     7561 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7621 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7681 atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc gctgcgctcg
     7741 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7801 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7861 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7981 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8041 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8101 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8161 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8221 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8281 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8341 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8401 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8461 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8521 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8581 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8641 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8701 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8761 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8821 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8881 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8941 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9001 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9061 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9121 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9181 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9241 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9301 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9361 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9421 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9481 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9541 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9601 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9721 gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc agttagggtg
     9781 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     9841 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9901 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     9961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    10021 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    10081 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca
    10141 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10201 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10261 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
    10321 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    10381 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
    10441 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    10501 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
    10561 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
    10621 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
    10681 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
    10741 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
    10801 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    10861 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    10921 acggccagtg aatt
//
