LOCUS       sequence-135651-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Fujii H, Fujita T
  TITLE     An enChIP system for the analysis of genome functions in budding 
            yeast.
  JOURNAL   Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 
            10.1093/biomethods/bpac025. eCollection 2022.
  PUBMED    36325175
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Methods Protoc."; date: "2022-10-17"; volume: "7(1):bpac025. doi"; 
            pages: " 10.1093/biomethods/bpac025. eCollection 2022"
COMMENT     Sequence Label: 3xFLAG-dCas9/pTEF1p-CYC1t
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..90
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     74..90
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             105..170
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ttttcttgtt ctattacaac tttttttact tcttgctcat tagaaagaaa gcatagcaat
       61 ctaatctaag ttttctagaa ctagtggatc ccccgggaaa aatggactac aaagaccatg
      121 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag ctcatggaca
      181 agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg
      241 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
      301 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc
      361 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
      421 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
      481 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
      541 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
      601 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
      661 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
      721 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
      781 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
      841 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
      901 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
      961 agcttcaact gagcaaagac acctacgatg atgatctcga caatctg
//
